Activities of selected medicinal plants against multi-drug resistant Gram-negative bacteria in Cameroon

Tchana, Marthe E S; Fankam, Aimé G.; Mbaveng, Armelle T.; Nkwengoua, Ernestine; Seukep, Jackson A.; Tchouani, Francesco K; Nyasse, Barthélémy; Kuete, Victor

doi:10.4314/ahs.v14i1.25

Cited by 31 publications

(10 citation statements)

References 22 publications

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“…The observed difference in antibacterial activities of extracts between the aforementioned pathogenic bacteria may be attributed to the difference in the outer membrane of isolates and may be due to the concentration difference of the secondary metabolite in the extract (Olila and Opuda-Asibo, 2001). Gram-negative bacteria possesses high permeability barrier for numerous antibiotic molecules (Marthe et al, 2014) similarly for these extracts, thus the tested extracts has shown lowest inhibition zone in almost all the Gram-negative bacteria during this experiment. Their periplasmic space also contains enzymes, which are capable of breaking down foreign molecules (Duffy and Power, 2001) and appears to be less susceptible to plant extracts than gram-positive bacteria.…”

Section: Discussionmentioning

confidence: 77%

“…Their periplasmic space also contains enzymes, which are capable of breaking down foreign molecules (Duffy and Power, 2001) and appears to be less susceptible to plant extracts than gram-positive bacteria. Furthermore, the weak antibacterial activities of this studied plants could be due to the resistance features of the studied bacterial strains against this plant extracts (Marthe et al, 2014). In comparison among ethanol, chloroform and hydro alcohol, ethanol showed slightly higher inhibition zone.…”

Section: Discussionmentioning

confidence: 88%

“…Ethno-botanical investigations have been found to offer important clues in the identification and development of traditionally used medicinal plants in to modern drugs (Kuete et al, 2008;Kuete, 2010). Plants have continued to play a dominant role as a source of medicinal compounds in the maintenance of human health since ancient times (Tchana et al, 2014). To the best of our knowledge, the in vitro antibacterial activity of P. lanceolata is being reported for the first time.…”

Section: Discussionmentioning

confidence: 99%

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In-Vitro Antibacterial Activity of Plantago Lanceolata Against Some Selected Standard Pathogenic Bacterial

Kassaw

Yohannes

Bizualem

2018

The International Journal of Biotechnology

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The leaves of Plantago lanceolata have been used for centuries to treat diseases. Currently there is an increasing incidence of multiple antibiotic resistances in microorganisms which is a major threat. Widespread overuse of antibiotics is one led to increasing clinical resistance of previously sensitive microorganisms and the emergence of previously uncommon infections. Thus all these calls for an urgent need to search new, effective and safe anti-bacterial agents. The objective of this study was to determine antibacterial potentials of extracts of P. lanceolata against standard pathogens bacteria. Active antibacterial compounds were extracted using three solvents. The antibacterial sensitivity activities of crude extracts were determined using agar well diffusion assay. MIC and MBC of each crude extracts were also determined using broth dilution method. The test showed various degrees of antibacterial activity towards each standard pathogenic microorganism with mean zone of inhibition ranges up to 18±2mm against S. typhi. The plant extract was showed as low 3±1mm to high 18±2mm diameter inhibition zone. Chloroform extract have the least inhibition zone while the ethanol have highest inhibition zone. The extract value of MIC was showed at the ranges of 12.5mg to 50mg while the MBC were showed at the range of 25mg to 50mg. The result of this study showed that leaves of P. lanceolata have considerable antibacterial potential. The claimed efficacy could be attributable to antibacterial activity of its components but its mode of action is unclear. Contribution/Originality: This study contributes in the existing literature by providing basic information for other researchers regarding the antibacterial efficacy potential of P. lanceolata leaves crude extract. Therefore, even though it needs further studies, leaves of P. lanceolata may be considered as a potential option to antibiotic regimens.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

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In-Vitro Antibacterial Activity of Plantago Lanceolata Against Some Selected Standard Pathogenic Bacterial

Kassaw

Yohannes

Bizualem

2018

The International Journal of Biotechnology

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“…The aqueous, chloroform, ethanol and hexane extracts were screened against MDR bacteria including A. baumannii , E. coli , P. aeruginosa and S. aureus . Tchana et al, (2014) performed similar type of study in Cameroon, in which it was confirmed that medicinal plants had the ability to encountered MDR bacteria [18]. …”

Section: Discussionmentioning

confidence: 98%

Alkanna tinctoria leaves extracts: a prospective remedy against multidrug resistant human pathogenic bacteria

Khan

Rahman

Qasim

et al. 2015

BMC Complement Altern Med

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BackgroundPlants are rich source of chemical compounds that are used to accomplish biological activity. Indigenously crude extracts of plants are widely used as herbal medicine for the treatment of infections by people of different ethnic groups. The present investigation was carried out to evaluate the biological potential of Alkanna tinctoria leaves extract from district Charsadda, Pakistan against multidrug resistant human pathogenic bacteria including Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.MethodsAnti-multi-drug resistant bacterial activity of aqueous, chloroform, ethanol and hexane extracts of Alkanna tinctoria leaves were evaluated by well diffusion method. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of different extracts were determined. Moreover qualitative phytochemicals screening of the studied extracts was performed.ResultsAll four selected bacteria including A. baumannii, E. coli, P. aeruginosa and S. aureus were categorized as multi-drug resistant (MDR) as they were found to be resistant to 13, 10, 19 and 22 antibiotics belonging to different groups respectively. All the four extract showed potential activity against S. aureus as compare to positive control antibiotic (Imipenem). Similarly among the four extracts of Alkanna tinctoria leaves, aqueous extract showed best activity against A. baumannii (10 ± 03 mm), P. aeruginosa (12 ± 0.5 mm), and S. aureus (14 ± 0.5 mm) as compare to Imipenem. The MICs and MBCs results also showed quantitative concentration of plant extracts to inhibit or kill MDR bacteria. When phytochemicals analysis was performed it was observed that aqueous and ethanol extracts showed phytochemicals with large number as well as volume, especially Alkaloides, Flavonoides and Charbohydrates.ConclusionThe undertaken study demonstrated that all the four extracts of Alkanna tinctoria leaves exhibited considerable antibacterial activity against MDR isolates. Finding from the current study will be helpful for further elucidation of lead molecules from Alkanna tinctoria leaves for future therapeutic use against MDR pathogens.

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“…The selection of C. intybus seeds was based on their traditional use in the Charsadda district of Pakistan. A previous study from Cameroon documented the use of medicinal plants against MDR bacteria (14). …”

Section: Discussionmentioning

confidence: 99%

Ethnomedicinal Cichorium intybus Seed Extracts: An Impending Preparation against Multidrug Resistant Bacterial Pathogens

Rahman

Khan

Qasim

et al. 2016

Jundishapur J Microbiol

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BackgroundThe present study was undertaken to analyze the phytochemical content and biological activity of Cichorium intybus seeds traditionally used in Charsadda, Pakistan against multidrug resistant (MDR) bacterial pathogens.ObjectivesThis study explored the qualitative and quantitative antibacterial potential of C. intybus. Further qualitative analysis of phytochemical content was performed.MethodsCichorium intybus seed extracts were prepared in aqueous, chloroform, ethanol, and hexane separately.ResultsAll the extracts of C. intybus seeds were screened for antibacterial activity and phytochemical content. Cichorium intybus seed extract showed considerable activity against MDR pathogenic bacteria. In the well diffusion method, aqueous extracts showed a higher zone of inhibition against Pseudomonas aeruginosa (16 mm ± 0.7 mm) and Acinetobacter baumannii (13 mm ± 0.5 mm), whereas chloroform, ethanol, and hexane extracts showed activity against P. aeruginosa (11 mm ± 0.3 mm, 12 mm ± 0.5 mm, and 11 mm ± 0 mm, respectively) as compared to Imipenem, a broad spectrum antibiotic. Minimum inhibitory concentration and minimum bactericidal concentration values for aqueous and ethanol extracts indicate that they were more effective against MDR bacteria. Phytochemical analysis revealed that aqueous and ethanol extracts were rich in alkaloids, carbohydrates, gallotannins, and triterpenoids, whereas chloroform and hexane extracts were more concentrated with phenolics, pseudotannins, saponins, and tannins. Cichorium intybus seed extract demonstrated potential activity against MDR human pathogenic bacteria.ConclusionsThe undertaken study has for the first time reported the effects of C. intybus seed extracts against MDR bacterial pathogens. Findings of the current study will be helpful for further elucidation of bioactive molecules for therapeutic use against MDR bacterial pathogens.

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Activities of selected medicinal plants against multi-drug resistant Gram-negative bacteria in Cameroon

Cited by 31 publications

References 22 publications

In-Vitro Antibacterial Activity of Plantago Lanceolata Against Some Selected Standard Pathogenic Bacterial

In-Vitro Antibacterial Activity of Plantago Lanceolata Against Some Selected Standard Pathogenic Bacterial

Alkanna tinctoria leaves extracts: a prospective remedy against multidrug resistant human pathogenic bacteria

Ethnomedicinal Cichorium intybus Seed Extracts: An Impending Preparation against Multidrug Resistant Bacterial Pathogens

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