Activities of Types A and B MAO and Catechol-O-methyltransferase in Blood Cells and Skin Fibroblasts of Normal and Chronic Schizophrenic Subjects

Groshong, Regina

doi:10.1001/archpsyc.1978.01770340048004

Cited by 68 publications

(18 citation statements)

References 72 publications

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“…In contrast, MAO-A activity in cultures from different individuals can differ over a wide range (often >10-fold to >100-fold), even in a single study (Costa et al 1980;Hotamisligil and Breakefield 1991). The range of fibroblast MAO-A specific activity within defined normal and patient populations is wide, and activity distributions invariably overlap extensively between groups (Groshong et al 1978;Breakefield and Edelstein 1980;Giller et al 1980Giller et al , 1984Singh et al 1979;Ramchand et al 1995). The range of fibroblast MAO-A specific activity within defined normal and patient populations is wide, and activity distributions invariably overlap extensively between groups (Groshong et al 1978;Breakefield and Edelstein 1980;Giller et al 1980Giller et al , 1984Singh et al 1979;Ramchand et al 1995).…”

Section: Richard M Denney · Helga Koch · Ian W Craigmentioning

confidence: 99%

Association between monoamine oxidase A activity in human male skin fibroblasts and genotype of the MAOA promoter-associated variable number tandem repeat

Denney¹,

Koch²,

Craig³

1999

Human Genetics

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Among fifteen male skin fibroblast cultures from eleven donors ranging in age from less than 1 year to 90 years old, the specific activity of monoamine oxidase A (MAO-A) differed 515-fold. Each culture had one of the two most common alleles (three or four 30-bp repeats) at the variable number tandem repeat locus positioned 1.2 kb upstream from MAOA exon 1 (uVNTR). The mean MAO-A activity in cultures with three uVNTR repeats was significantly lower than that in cultures with four repeats (1.6 +/- 1.1 and 13 +/- 12 nmol/h per milligram, respectively; P=0.032). MAO-A expression was confined to a cell sub-population varying from 0.5% to 90% of cells in different cultures. The mean specific activity in MAO-A+ cells (whole culture specific activity divided by the proportion of immunopositive cells) was lower for cultures with three repeats than for those with four (7.2 +/- 3.1 and 23.9 +/- 9.5 nmol/h per milligram protein, respectively; P=0.0013), with no overlap in activity between genotypes. Finding lower MAO-A activity in cultures with three uVNTR repeats compared to those with four is consistent with published evidence that MAO-A promoter constructs bearing three repeats have lower transcriptional activity in transfected neuroblastoma and choriocarcinoma cells. The uVNTR genotype may be a common genetic determinant of significant individual differences in oxidizing capacity for critical MAO-A substrates, which include serotonin, norepinephrine, and tyramine.

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Section: Richard M Denney · Helga Koch · Ian W Craigmentioning

confidence: 99%

Association between monoamine oxidase A activity in human male skin fibroblasts and genotype of the MAOA promoter-associated variable number tandem repeat

Denney¹,

Koch²,

Craig³

1999

Human Genetics

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“…Friedman et al (22) found no difference in monoamine oxidase activity between patients and normal control subjects, using tryptamine as substrate. In contrast, Bailey et al (23), Groshong et al (24) and Schildkraut et al (25) did not find a lower platelet monoamine oxidase activity in schizophrenic patients using different substrates. Other authors, such as Balmaker et al (17), reported slightly higher monoamine oxidase activity in schizophrenic patients.…”

Section: Discussionmentioning

confidence: 87%

Determination of Platelet Monoamine Oxidase by New Continuous Spectrophotometric Method

Ivanović

Majkić-Singh²

1988

Clinical Chemistry and Laboratory Medicine

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Summary:A simple, continuous Spectrophotometric method for the determination of tissue monoamine oxidase based on the oxidation of 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) using peroxidase has already been described (Ivanovic, I. & Majkic-Singh, N. (1986) Fresenius Z. Anal. Chem. 324, 307). In the present study the method is optimised for platelet monoamine oxidase assay and applied to healthy persons and schizophrenic patients. The obtained data were statistically analysed. The continuous ABTS method is sensitive, precise (CV below 6. The reference values for the activity of human platelet monoamine oxidase by the new continuous ABTS method are 25 to 42 U/g protein (x = 33.2 U/g protein, CV = 15.5%, n = 67). No differences were found between females and males, or between three age groups ranging from 21 to 52 years. The patients with chronic (n = 76) or acute (n = 17) schizophrenia had significantly lower monoamine oxidase activities compared with normal values (p < 0.005), which indicates that platelet monoamine oxidase can be a possible marker for schizophrenic diseases.

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“…It is known that fibroblasts from rabbit cornea (Fujita et al, 1987) and mouse skin (Fujita et al, 1988;Fujita, 1989), like macrophages, exhibit strong endocytic activity. Furthermore, the presence of COMT has been reported in fibroblasts in guinea pig ventricle (Jacobowitz, 1972), human skin (Groshong et al, 1978), and rat and mouse breast tissue (Amin et al, 1983). This apparent diversity may stem from a species-specific difference or be an indication of a diverse expression of COMT in various classes of macrophages, possibly in response to inflammation or other changes in the micro-environment (Sontheimer, 1989;Speer and Gahr, 1989).…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical Evidence for the Site of O-methylation in Rat Dental Pulp

1991

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Immunocytochemical observations by light and electron microscopy of catechol-O-methyltransferase (COMT) were conducted in dental pulp by use of a specific antibody to soluble rat-liver COMT and the peroxidase-antiperoxidase technique. Immunoreactive deposits were found in macrophages. The pattern of localization suggests that COMT may function in extraneuronal inactivation of catecholamines in dental pulp.

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Activities of Types A and B MAO and Catechol-O-methyltransferase in Blood Cells and Skin Fibroblasts of Normal and Chronic Schizophrenic Subjects

Cited by 68 publications

References 72 publications

Association between monoamine oxidase A activity in human male skin fibroblasts and genotype of the MAOA promoter-associated variable number tandem repeat

Association between monoamine oxidase A activity in human male skin fibroblasts and genotype of the MAOA promoter-associated variable number tandem repeat

Determination of Platelet Monoamine Oxidase by New Continuous Spectrophotometric Method

Immunocytochemical Evidence for the Site of O-methylation in Rat Dental Pulp

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