Activity against plant pathogenic fungi of Lactobacillus plantarum IMAU10014 isolated from Xinjiang koumiss in China

Abstract

Thirty-five of the 77 colonies of lactic acid bacteria isolated from koumiss showed antifungal activity against Botrytis cinerea, when tested by the Poison Food Technique. Of these, the most promising isolate having a broad spectrum of antifungal activity, including against Botrytis cinerea, Alternaria solani, Phytophthora drechsleri Tucker, Fusarium oxysporum and Glomerella cingulata, was identified as Lactobacillus plantarum IMAU10014. The effect of pH, temperature and protease on the antifungal activity of L. plantarum IMAU10014 was determined. The activity substance was heat stable. The maximum antifungal activity was observed at pH 4.0. When the supernatant was adjusted to pH 8.0, the activity was lost irreversibly. However, after enzymatic treatment of supernatant with trypsin, neutral protease and proteinase K, loss of part of the antifungal activity was observed which indicated there are other substances in addition to the proteinaceous substance with the antifungal activity.

Production and Characterization of Antifungal Compounds Produced by Lactobacillus plantarum IMAU10014

“…But there are few reports on the activity against plant pathogenic fungi and the antifungal principles characteristics of L. plantarum that we know of thus far [16]. Phytophthora are pathogens that infect a wide range of plant species.…”

Biological control of bacterial plant diseases with Lactobacillus plantarum strains selected for their broad‐spectrum activity

“…This strategy has been previously used to screen for BCA in other pathosystems (Haidar et al, ). As some LAB strains have shown antagonistic activity against bacterial and fungal plant pathogens (Fhoula et al, ; Trias, Bañeras, Montesinos, & Badosa, ; Visser et al, ; Wang et al, ) and biocontrol efficacy on plants (Roselló et al, ; Roselló et al, ; Shrestha, Kim, & Park, ; Tsuda et al, ), plant‐associated LAB have been considered as candidates for developing BCA.…”

Alterations in honey bee gut microorganisms caused by Nosema spp. and pest control methods

“…Our results show that application of the probiotic bacterium L. plantarum mitigated alterations in bee gut microbial communities after Nosema infection, suggesting its potential use in the biocontrol of bee pests. The biocontrol mode of action was not revealed in this study, but may be linked to production of antifungal compounds such as antimicrobial peptides and short‐chain fatty acids . Another possible mode of action of L. plantarum is the strong capacity to acidify its environment because of the high levels of lactic acid produced, efficiently impeding infection by bee pathogens like P. larve .…”