Activity and adaptation of nitrilotriacetate (NTA)-degrading bacteria: field and laboratory studies

McFeters, Gordon A.; Egli, Thomas; Wilberg, E.; Alder, Alfredo C.; Schneider, René Peter; Suozzi, M.; Giger, Walter

doi:10.1016/0043-1354(90)90137-u

Cited by 35 publications

(18 citation statements)

References 33 publications

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“…These results showed that speci®c activity in terms of citrate and nitrate consumption was signi®cantly higher for the cells in bio®lm form. This is in accordance with the results of several authors who have reported that bacteria associated with a solid surface exhibit higher uptake rates (Paerl and Merkel 1982;McFeters et al 1990). It is well known that cells grown on solid surfaces have different properties from those of planktonic cells.…”

Section: Discussionsupporting

confidence: 93%

“…Marshall and Goodman (1994) suggested that differences experienced by bacteria at surfaces may alter the expression of certain genes in either a positive or negative manner and hence, may alter the overall physiological characteristics of the immobilized organisms. McFeters et al (1990) reported that bacteria attached to sand particles had a shorter lag time and higher speci®c activity in the degradation of nitrilotriacetate than bacteria in the bulk aqueous phase. Vandevivere and Kirchman (1993) have shown that exopolymer production by bacterial cells is stimulated at a solid surface.…”

Section: Introductionmentioning

confidence: 99%

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Metabolism of Alcaligenes denitrificans in biofilm vs planktonic cells

Teixeira

Oliveira

2002

J Appl Microbiol

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Aims:To compare the effect of phosphorous concentration (200 mg P l ±1 and 20 mg P l ±1 ) on the denitrifying ef®ciency of Alcaligenes denitri®cans when in the form of planktonic cells or in the form of a bio®lm, and to select the most adequate C/N ratio. Methods and Results: Two types of assays were carried out: with planktonic cells and with cells in bio®lm form. Anoxic bottles with the appropriate C/N and phosphorous concentration were incubated at 30°C and submitted to orbital shaking at 150 rev min ±1 . The speci®c activity of cells in bio®lm form, in terms of substrate consumption, was signi®cantly higher than cells in planktonic form. With regard to the effect of increasing phosphorous concentration, an increase in speci®c activity was also only evident when the cells were in bio®lm form. Conclusions: The two forms showed different performances and phosphorous concentration only markedly affected the bio®lm form. Signi®cance and Impact of the Study: The importance of the C/N/P ratio in the denitri®cation process is demonstrated. As there was no report in the literature about the stoichiometric relationship of heterotrophic denitri®cation with citrate, its stoichiometry, including the requirement for cell synthesis, was determined.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Metabolism of Alcaligenes denitrificans in biofilm vs planktonic cells

Teixeira

Oliveira

2002

J Appl Microbiol

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“…However, it is presently n o t known how environmental conditions and nutritional status affect the regulation of both N T A -M O and I D A -DH in these bacteria. Preliminary data suggested that the capacity t o degrade NTA in the environment might lie regulated via induction/repression rather than by enrichment of competent microbes (McFeters e t al., 1990). Therefore, in Cb.…”

Section: Introductionmentioning

confidence: 99%

Growth and regulation of enzyme synthesis in the nitrilotriacetic acid (NTA)-degrading bacterium Chelatobacter heintzii ATCC 29600

Bally

Wilberg²,

Kühni³

et al. 1994

Microbiology

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In the aerobic bacterium Chelatobacter heintzii, growth and regulation of enzymes involved in nitrilotriacetic acid (NTA) degradation have been investigated in chemostat culture during cultivation with glucose, NTA or mixtures thereof. In batch culture ,urn,, with NTA was 018 h-I and with glucose 0.22 h-I. Growth yields for both substrates were reduced at low dilution rates. During growth with NTA specific activity of the NTA monooxygenase (NTA-MO) exhibited a maximum at D = 003 h-I and gradually decreased with increasing dilution rates. In glucose-grown cells the specific activity as well as immunologically detectable NTA-MO protein was always close to the detection limit. During cultivation with different mixtures of NTA and glucose at a dilution rate of 006 h-I, both substrates were utilized simultaneously, irrespective of the NTA/glucose ratio and the presence of excess ammonia. Synthesis of both NTA-MO and iminodiacetic acid dehydrogenase became induced when NTA contributed to more than approximately 1-3% of the total carbon in the substrate mixture supplied. However, NTA was also degraded when the proportion of NTA in the mixture was lower than 1 %, which is consistent with the low constitutive level of expression for NTA-MO observed. Results are discussed with respect to NTA biodegradation during sewage treatment and in ecosystems.

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“…The first reported observation of this effect was made in 1943, where it was found that bacterial activity increased in the presence of glass surfaces, particularly with low nutrient concentrations (54). Since this first study, researchers have examined how various materials, including glass and polymer surfaces (10, 11, 24-26, 40, 44, 47), silicone surfaces (52), ceramic surfaces (32), dialysis membranes (18), activated carbon (7), clays (43), sands (31,48), estuary particles (19), and ion exchange resins (46), affect the metabolic activity of attached bacteria.…”

mentioning

confidence: 99%

Variation in Bacterial ATP Level and Proton Motive Force Due to Adhesion to a Solid Surface

Hong

Brown

2009

Appl Environ Microbiol

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Bacterial adhesion to natural and man-made surfaces can be beneficial or detrimental, depending on the system at hand. Of vital importance is how the process of adhesion affects the bacterial metabolic activity. If activity is enhanced, this may help the cells colonize the surface, whereas if activity is reduced, it may inhibit colonization. Here, we report a study demonstrating that adhesion of both Escherichia coli and Bacillus brevis onto a glass surface resulted in enhanced metabolic activity, assessed through ATP measurements. Specifically, ATP levels were found to increase two to five times upon adhesion compared to ATP levels in corresponding planktonic cells. To explain this effect on ATP levels, we propose the hypothesis that bacteria can take advantage of a link between cellular bioenergetics (proton motive force and ATP formation) and the physiochemical charge regulation effect, which occurs as a surface containing ionizable functional groups (e.g., the bacterial cell surface) approaches another surface. As the bacterium approaches the surface, the charge regulation effect causes the charge and pH at the cell surface to vary as a function of separation distance. With negatively charged surfaces, this results in a decrease in pH at the cell surface, which enhances the proton motive force and ATP concentration. Calculations demonstrated that a change in pH across the cell membrane of only 0.2 to 0.5 units is sufficient to achieve the observed ATP increases. Similarly, the hypothesis indicates that positively charged surfaces will decrease metabolic activity, and results from studies of positively charged surfaces support this finding.Bacterial adhesion and biofilm formation are important to a wide array of fields, such as environmental, chemical, and biomedical engineering; food processing; materials science; marine science; and ecology and environmental sciences. A key consideration in the development of a biofilm is the initial interaction between the bacterium and the substrata and the way in which these interactions affect the metabolic activity of the cells. If the metabolic activity increases, this would help the cells colonize the surface, whereas if the metabolic activity decreases, then the cells may become inactive or die.There have been a number of studies that have examined the effects of attachment on bacterial metabolic activity. The first reported observation of this effect was made in 1943, where it was found that bacterial activity increased in the presence of glass surfaces, particularly with low nutrient concentrations (54). Since this first study, researchers have examined how various materials, including glass and polymer surfaces (10, 11, 24-26, 40, 44, 47), silicone surfaces (52), ceramic surfaces (32), dialysis membranes (18), activated carbon (7), clays (43), sands (31, 48), estuary particles (19), and ion exchange resins (46), affect the metabolic activity of attached bacteria.Different mechanisms have been examined in an attempt to explain how surfaces affect bacterial metabo...

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Activity and adaptation of nitrilotriacetate (NTA)-degrading bacteria: field and laboratory studies

Cited by 35 publications

References 33 publications

Metabolism of Alcaligenes denitrificans in biofilm vs planktonic cells

Metabolism of Alcaligenes denitrificans in biofilm vs planktonic cells

Growth and regulation of enzyme synthesis in the nitrilotriacetic acid (NTA)-degrading bacterium Chelatobacter heintzii ATCC 29600

Variation in Bacterial ATP Level and Proton Motive Force Due to Adhesion to a Solid Surface

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