Activity and isoenzyme composition of vacuolar peroxidase in the roots of red beet at different stages of development and upon changes in storage conditions

Nimaeva, O. D.; Pradedova, E. V.; Salyaev, R. K.

doi:10.1134/s1021443714030108

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…For instance, in ower buds of potted lemon tree, POD activity did not signi cantly change during the winter rest period, but increased 4-5 fold upon reaching SD release (Kasraoui et al 2014). In beetroot, POD activity was the highest during the growth stage and the lowest during the dormant period (Nimaeva et al 2014;Pradedova et al 2014). In addition, during germination, the POD activity of Berberis seeds increased (Belwal et al 2015), whereas POD activity of mung bean (Vigna radiata L.) increased initially but then decreased (Singh et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Functional analysis of the wheat class III peroxidase gene TaPer12-3A in seed dormancy and germination

Gao,

Jiang,

Yang

et al. 2023

Preprint

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Class III peroxidases (PODs) perform crucial functions in various developmental processes and in response to biotic and abiotic stresses. However, the specific roles of class III POD genes in wheat seed dormancy (SD) and germination remain elusive. Here, we identified a wheat class III POD gene, named TaPer12-3A, based on transcriptome data and expression analysis. TaPer12-3A showed decreasing and increasing expression trends with SD acquisition and release, respectively, indicating a significant association with SD and germination. It was highly expressed in wheat seeds and localized in the endoplasmic reticulum and cytoplasm. Germination tests indicated that TaPer12-3A negatively regulated SD and positively mediated germination in transgenic Arabidopsis and rice lines as well as in a wheat mutant mutagenized with ethyl methane sulfonate (EMS) in Jing 411 background. Further studies demonstrated that TaPer12-3A regulated SD and germination by interacting with gibberellin and abscisic acid biosynthesis, catabolism, and signaling pathways in transgenic rice seeds. These findings not only provide new insights for future functional analysis of TaPer12-3A in regulating wheat SD and germination but also contribute to understanding the complex regulatory mechanism involved in these processes.

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Section: Discussionmentioning

confidence: 99%

Functional analysis of the wheat class III peroxidase gene TaPer12-3A in seed dormancy and germination

Gao,

Jiang,

Yang

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…For instance, in flower buds of potted lemon tree, POD activity did not significantly change during the winter rest period, but increased 4–fivefold upon reaching dormancy release [ 30 ]. In beetroot, POD activity was the highest during the growth stage and the lowest during the dormant period [ 31 , 32 ]. In addition, during germination, the POD activity of Berberis seeds increased [ 33 ], whereas that of mung bean ( Vigna radiata ) increased initially but then decreased [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Functional analysis of a wheat class III peroxidase gene, TaPer12-3A, in seed dormancy and germination

Gao,

Jiang,

Yang

et al. 2024

BMC Plant Biol

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Background Class III peroxidases (PODs) perform crucial functions in various developmental processes and responses to biotic and abiotic stresses. However, their roles in wheat seed dormancy (SD) and germination remain elusive. Results Here, we identified a wheat class III POD gene, named TaPer12-3A, based on transcriptome data and expression analysis. TaPer12-3A showed decreasing and increasing expression trends with SD acquisition and release, respectively. It was highly expressed in wheat seeds and localized in the endoplasmic reticulum and cytoplasm. Germination tests were performed using the transgenic Arabidopsis and rice lines as well as wheat mutant mutagenized with ethyl methane sulfonate (EMS) in Jing 411 (J411) background. These results indicated that TaPer12-3A negatively regulated SD and positively mediated germination. Further studies showed that TaPer12-3A maintained H2O2 homeostasis by scavenging excess H2O2 and participated in the biosynthesis and catabolism pathways of gibberellic acid and abscisic acid to regulate SD and germination. Conclusion These findings not only provide new insights for future functional analysis of TaPer12-3A in regulating wheat SD and germination but also provide a target gene for breeding wheat varieties with high pre-harvest sprouting resistance by gene editing technology.

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“…In beetroot, POD activity was the highest during the growth stage and the lowest during the dormant period [33,34]. In addition, during germination, the POD activity of Berberis seeds increased [35], whereas that of mung bean (Vigna radiata) increased initially but then decreased [36].…”

Section: Discussionmentioning

confidence: 99%

Functional analysis of a wheat class III peroxidase gene, TaPer12-3A, in seed dormancy and germination

Gao,

Jiang,

Yang

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Class III peroxidases (PODs) perform crucial functions in various developmental processes and responses to biotic and abiotic stresses. However, their roles in wheat seed dormancy (SD) and germination remain elusive. Results Here, we identified a wheat class III POD gene, named TaPer12-3A, based on transcriptome data and expression analysis. TaPer12-3A showed decreasing and increasing expression trends with SD acquisition and release, respectively. It was highly expressed in wheat seeds and localized in the endoplasmic reticulum and cytoplasm. Germination tests were performed using transgenic Arabidopsis and rice lines as well as wheat mutant mutagenized with ethyl methane sulfonate (EMS) in a Jing 411 (J411) background. These tests indicated that TaPer12-3A negatively regulated SD and positively mediated germination. Further studies showed that TaPer12-3A maintained H2O2 homeostasis by scavenging excess H2O2 and participated in the biosynthesis and catabolism of gibberellic acid and abscisic acid to regulate SD and germination. Conclusion These findings not only provide new insights for future functional analysis of TaPer12-3A in regulating wheat SD and germination but also provide a target gene for breeding wheat varieties with high SD and pre-harvest sprouting resistance by gene editing technology.

show abstract

Activity and isoenzyme composition of vacuolar peroxidase in the roots of red beet at different stages of development and upon changes in storage conditions

Cited by 5 publications

References 24 publications

Functional analysis of the wheat class III peroxidase gene TaPer12-3A in seed dormancy and germination

Functional analysis of the wheat class III peroxidase gene TaPer12-3A in seed dormancy and germination

Functional analysis of a wheat class III peroxidase gene, TaPer12-3A, in seed dormancy and germination

Functional analysis of a wheat class III peroxidase gene, TaPer12-3A, in seed dormancy and germination

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