Activity and survival of tribromophenol-degrading bacteria in a contaminated desert soil

Ronen, Zeev; Vasiluk, Luba; Abeliovich, Aharon; Nejidat, Ali

doi:10.1016/s0038-0717(00)00080-8

Cited by 47 publications

(19 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was recently found that Achromobacter piechaudii, isolated from desert soil contaminated with chemical industry waste, reductively dehalogenated 2,4,6-TBP to phenol. 19) Cells adhering to chalk were more active in 2,4,6-TBP degradation than cells in suspension. 20) The bacterium, however, was not able to metabolize mono-or dibromophenols.…”

Section: Discussionmentioning

confidence: 90%

See 1 more Smart Citation

Biodegradation of 2,4,6-Tribromophenol byOchrobactrumsp. Strain TB01

Yamada

Takahama

Yamada

2008

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 90%

“…20) The bacterium, however, was not able to metabolize mono-or dibromophenols. 19,21) These results suggest that there are a number of bromophenoldegrading bacteria in various ecological niches, and that there is a diversity of dehalogenating enzymes with various substrate specificities.…”

Section: Discussionmentioning

confidence: 91%

Biodegradation of 2,4,6-Tribromophenol byOchrobactrumsp. Strain TB01

Yamada

Takahama

Yamada

2008

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

“…This strain is capable of degrading 2,4,6‐tribromophenol (TBP) and displays kanamycin resistance as a selective marker. The isolation and characterization of this bacterial strain was previously described by Ronen et al. (2000).…”

Section: Bacterial Strain and Growth Conditionsmentioning

confidence: 99%

“…TBPZ‐N61 cells were grown in mineral medium supplemented with TBP and kanamycin for 16 or 90 h. The cells were suspended (7 × 10 7 cells ml −1 ) in 100 ml phosphate buffer (10 m m , pH 7·2), containing 0·69% w/v white chalk or 1% w/v grey chalk, and incubated in a rotary shaker (150 rev min −1 ) for 1 h. Thereafter, TBP was added to a final concentration of 100 mg l −1 . At different intervals, samples were withdrawn, centrifuged (15 115 g , 5 min) to discard the chalk and TBP concentration was measured in the supernatant using ‘high performance liquid chromatography (HPLC) as described by Ronen et al. (2000).…”

Section: Activity Of Adhered Bacterial Cellsmentioning

confidence: 99%

Degradation of 2,4,6-tribromophenol by bacterial cells attached to chalk collected from a contaminated aquifer

Nejidat¹,

Saadi²,

Ronen³

2004

J Appl Microbiol

View full text Add to dashboard Cite

Aim: To investigate the factors governing the adhesion and activity of the 2,4,6-tribromophenol (TBP) degrading bacterium Achromobacter piechaudii TBPZ-N61 on chalk from a contaminated aquifer. Methods and Results: Adhesion kinetics of TBPZ-N61 to grey and white chalk from a polluted fractured chalk aquifer was tested in a batch system. Both grey and white chalk contain ca 80% CaCO 3 , while grey chalk contains more organic matter (2AE4%) than the white chalk (0AE3%) and also contains Dolmite and Clinoptilolite. Adhesion of the bacterial cells to the chalk particles (<0AE2 mm) occurred rapidly (96% of the cells within 15 min). Langmuirfitted adhesion isotherms suggest that cells in the stationary phase, which are more hydrophobic, adhere to both grey and white chalk more efficiently than cells in the logarithmic growth phase. Increasing the pH (from 6AE7 to 8AE1) caused a significant reduction in cell adhesion to the chalk. Activity of attached cells was evaluated in both batch and column experiments. Logarithmic cells adhering to white and grey chalk were more active in TBP degradation than cells in suspension. In column experiments, significant TBP degradation was retained up to 30 days after a single injection of TBPZ cells. Thereafter, activity was fully recovered by amendment of yeast extract. Chalk surfaces that were incubated in situ in contaminated groundwater for 20 days still allowed the adhesion and activity of TBPZ cells. Conclusions: Taken together, our results show that bacteria adhere efficiently to specific sites on the chalk surfaces, and that sustained bacterial activity of the attached cells can be achieved by adding a carbon source such as yeast extract which also overcome toxic constituents that may occur in some chalk types. Significance and Impact of the Study: Bioremediation of TBP-contaminated chalk aquifers is made possible by the injection of bacterial cultures.

show abstract

“…After intensive studies on terrestrial microorganisms, consequent attention has been focused on other ecosystems, especially those subject to extreme environmental conditions such as the desert [1], hot springs [2], and the sea [3]. Marine microorganisms live in a biological competitive environment with unique conditions of salinity, pressure, nutrition, temperature (low or local high temperature), and complex relations with other different creatures [4].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of marine bacteria from Malaysian coastal area

Mazalan

Zain

Hamzah

2012

2012 IEEE Symposium on Humanities, Science and Engineering Research

View full text Add to dashboard Cite

The marine environment is an abundant resource for the isolation of less exploited microorganisms. The unexploited habitats in the sea exist with unique characteristics. Additionally, the potential contribution of marine sources to the discovery of new bioactive molecules was recently recognized. In order to explore marine microorganisms with medical potential, marine bacteria were isolated from sea water collected from different areas of Pulau Kapas Beach and Pantai Batu Burok, Terengganu. The growth curve and the antimicrobial activities of these bacteria were investigated. Organic extracts (hexane, ethyl acetate, and butanol) of marine bacterial fermentation were screened for antimicrobial activities using disk diffusion method. The results revealed that the marine bacteria D7 entered the stationary phase at 24 hr of growth. The ethyl acetate extracts displayed antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Klebsiella pneumoniae, but possessed no effect on bacterium Escherichia coli, and yeasts Candida albicans. These marine bacteria are expected to be potential resources of natural antibiotic products.

show abstract

Activity and survival of tribromophenol-degrading bacteria in a contaminated desert soil

Cited by 47 publications

References 18 publications

Biodegradation of 2,4,6-Tribromophenol byOchrobactrumsp. Strain TB01

Biodegradation of 2,4,6-Tribromophenol byOchrobactrumsp. Strain TB01

Degradation of 2,4,6-tribromophenol by bacterial cells attached to chalk collected from a contaminated aquifer

Antimicrobial activity of marine bacteria from Malaysian coastal area

Contact Info

Product

Resources

About