Activity and synergistic interactions of stilbenes and antibiotic combinations against bacteria in vitro

Kumar, Santosh; Siji, J. V.; Nambisan, Bala; Mohandas, C.

doi:10.1007/s11274-012-1124-0

Cited by 43 publications

(33 citation statements)

References 17 publications

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“…resveratrol in grapes) which play a role in defending the host against microbial attack [35]. Stilbene variants such as hydroxystilbene and isopropylstilbene are potential antimicrobials and show a synergistic effect when applied together with antibiotics to inhibit and kill bacteria [36]. Our data consistently demonstrated that both stilbene derivatives (UC-27 and UC-28) showed bactericidal effect towards S. aureus at 200 μM.…”

Section: Resultssupporting

confidence: 56%

Discovery of potential anti-infectives against Staphylococcus aureus using a Caenorhabditis elegans infection model

Kong

Yehye

Rahman

et al. 2014

BMC Complement Altern Med

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BackgroundThe limited antibiotic options for effective control of methicillin-resistant Staphylococcus aureus infections has led to calls for new therapeutic approaches to combat this human pathogen. An alternative approach to control MRSA is through the use of anti-infective agents that selectively disrupt virulence-mediated pathways without affecting microbial cell viability or by modulating the host natural immune defenses to combat the pathogen.MethodsWe established a C. elegans – S. aureus liquid-based assay to screen for potential anti-infectives against S. aureus. The assay was utilized to screen 37 natural extracts and 29 synthetic compounds for the ability to extend the lifespan of infected nematodes. Disc diffusion and MIC microdilution tests were used to evaluate the anti-microbial properties of these natural extracts and synthetic compounds whilst in vivo bacterial CFU within the C. elegans gut were also enumerated.ResultsWe screened a total of 37 natural extracts and 29 synthetic compounds for anti-infective properties. The screen successfully revealed 14 natural extracts from six plants (Nypa fruticans, Swietenia macrophylla, Curcuma longa, Eurycoma longifolia, Orthosiphon stamineus and Silybum eburneum) and one marine sample (Faunus ater) that improved the survival of S. aureus-infected worms by at least 2.8-fold as well as 14 synthetic compounds that prolonged the survival of S. aureus-infected nematodes by 4-fold or greater. An anti-microbial screen of all positive hits demonstrated that 8/28 hits had no effect on S. aureus growth. Of these 8 candidates, 5 of them also protected the worms from MRSA infection. We also noted that worms exposed to N. fruticans root and O. stamineus leaf extracts showed reduced intestinal colonization by live S. aureus. This suggests that these extracts could possibly activate host immunity to eliminate the bacteria or interfere with factor/s that prevents pathogen accumulation.ConclusionWe have successfully demonstrated the utility of this liquid-based screen to identify anti-infective substances that prolong S. aureus-infected host survival without affecting bacterial cell viability.

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Section: Resultssupporting

confidence: 56%

Discovery of potential anti-infectives against Staphylococcus aureus using a Caenorhabditis elegans infection model

Kong

Yehye

Rahman

et al. 2014

BMC Complement Altern Med

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“…Of course, several successful plant pathogens are nevertheless able to circumvent the toxic effects of these plant metabolites which can be observed from our study in both Bacillus cereus ATCC 11778 and Acinetobacter baumannii ATCC 19606, demonstrating no susceptibility to the pterostilbene compound. Combine antibiotic therapy may produce synergistic effects in the treatment of bacterial infection [ 26 ] as supported by previous study that phytochemical has the ability to delay the emergence of antimicrobial resistance [ 19 ]. Previous in vitro studies have reported synergistic combinations of various stilbene compounds, such as ε-viniferin and johorenol A against MRSA, both ATCC and clinical strains [ 3 , 4 ].…”

Section: Discussionmentioning

confidence: 91%

Bactericidal Effect of Pterostilbene Alone and in Combination with Gentamicin against Human Pathogenic Bacteria

2017

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The antibacterial activity of pterostilbene in combination with gentamicin against six strains of Gram-positive and Gram-negative bacteria were investigated. The minimum inhibitory concentration and minimum bactericidal concentration of pterostilbene were determined using microdilution technique whereas the synergistic antibacterial activities of pterostilbene in combination with gentamicin were assessed using checkerboard assay and time-kill kinetic study. Results of the present study showed that the combination effects of pterostilbene with gentamicin were synergistic (FIC index < 0.5) against three susceptible bacteria strains: Staphylococcus aureus ATCC 25923, Escherichia coli O157 and Pseudomonas aeruginosa 15442. However, the time-kill study showed that the interaction was indifference which did not significantly differ from the gentamicin treatment. Furthermore, time-kill study showed that the growth of the tested bacteria was completely attenuated with 2 to 8 h treatment with 0.5 × MIC of pterostilbene and gentamicin. The identified combinations could be of effective therapeutic value against bacterial infections. These findings have potential implications in delaying the development of bacterial resistance as the antibacterial effect was achieved with the lower concentrations of antibacterial agents.

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“…They occur naturally in various plant families, such as the Cyperaceae, Dipterocarpaceae, Fabaceae, Gnetaceae, Moraceae, Polygonaceae, and Vitaceae, whereas grapes and related products are considered to be the most important dietary sources of these substances [10]. In previous studies, antimicrobial effect of model stilbene resveratrol and its related structures (e.g., piceatannol, pterostilbene, trans-piceid, and trans-ε-viniferin) has been reported against various food and human pathogenic microorganisms, such as Acetobacter aceti, Acetobacter oeni, Bacillus cereus, Bacillus subtilis, Dekkera bruxellensis, Escherichia coli, Listeria innocua, Listeria monocytogenes, Pseudomonas fluorescens, Pseudomonas aeruginosa, Streptococcus spp., Zygosaccharomyces bailii, and Zygosaccharomyces rouxii [11][12][13][14][15]. The in vitro growth-inhibitory effect of (E)-3-hydroxy-5-methoxystilbene, oxyresveratrol, pterostilbene, and resveratrol has also previously been described against S. aureus and Staphylococcus epidermidis [15][16][17][18].…”

Section: Introductionmentioning

confidence: 87%

The relationship between structure and in vitro antistaphylococcal effect of plant-derived stilbenes

Zakova

Rondevaldova

Bernardos

et al. 2018

AMicr

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Staphylococcus aureus is a major human pathogen that is responsible for both hospital-and community-acquired infections. Stilbenes are polyphenol compounds of plant origin known to possess a variety of pharmacological properties, such as antibacterial, antiviral, and antifungal effects. This study reports the in vitro growth-inhibitory potential of eight naturally occurring stilbenes against six standard strains and two clinical isolates of S. aureus, using a broth microdilution method, and expressing the results as minimum inhibitory concentrations (MICs). Pterostilbene (MICs = 32-128 μg/ml), piceatannol (MICs = 64-256 μg/ml), and pinostilbene (MICs = 128 μg/ml) are among the active compounds that possess the strongest activity against all microorganisms tested, followed by 3′-hydroxypterostilbene, isorhapontigenin, oxyresveratrol, and rhapontigenin with MICs 128-256 μg/ml. Resveratrol (MIC = 256 μg/ml) exhibited only weak inhibitory effect. Furthermore, structure-activity relationships were studied. Hydroxyl groups at ortho-position (B-3′ and -4′) played crucial roles for the inhibitory effect of hydroxystilbene piceatannol. Compounds with methoxy groups at ring A (3′-hydroxypterostilbene, pinostilbene, and pterostilbene) produced stronger effect against S. aureus than their analogues (isorhapontigenin and rhapontigenin) with methoxy groups at ring B. These findings provide arguments for further investigation of stilbenes as prospective leading structures for development of novel antistaphylococcal agents for topical treatment of skin infections.

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Activity and synergistic interactions of stilbenes and antibiotic combinations against bacteria in vitro

Cited by 43 publications

References 17 publications

Discovery of potential anti-infectives against Staphylococcus aureus using a Caenorhabditis elegans infection model

Discovery of potential anti-infectives against Staphylococcus aureus using a Caenorhabditis elegans infection model

Bactericidal Effect of Pterostilbene Alone and in Combination with Gentamicin against Human Pathogenic Bacteria

The relationship between structure and in vitro antistaphylococcal effect of plant-derived stilbenes

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