Activity and Toxicity of Eleutherine palmifolia (L.) Merr. Extract on BALB/c Mice Colitis-Associated Colon Cancer Model

Abstract

Objective: Eleutherine palmifolia (L.) Merr. extract (EPE) containing isoliquiritigenin and oxyresveratrol is believed to be an anticancer agent. This study evaluates colon histopathology, TNF-α, TGF-β, and hepatotoxicity on BALB/c mice colitis-associated colon cancer (CAC) model treated with EPE. Methods: In vivo study was performed on BALB/c mice CAC model induced by 10 mg/kgBW AOM on the first day followed by administration that each cycle consisted of 5% DSS in water for seven days and regular water for seven days. The indicators of the formation of CAC were observed by a fecal occult blood test (FOBT) and serum amyloid α (SAA) test. The treatment was conducted once a week started from the seventh week up to the twentieth week with six treatment groups: I was administrated by regular water only (negative control), II was administrated by AOM and DSS only (positive control), III was administrated by doxorubicin, IV-VI were treated by EPE (0.25 mg/kg BW, 0.50 mg/kg BW, and 1.00 mg/kg BW) respectively. The colon and liver’s histopathology was observed using hematoxylin-eosin (HE) staining, TNF-α with immunohistochemistry (IHC), and level measurement of TGF-β colon with ELISA reader. The data were used one-way ANOVA followed by post hoc as statistical analysis. Results: The administration of EPE increased the expression of TNF-α, the total of goblet cells of the colon, and decreased the level of TGF-β. Administration of EPE 0.50 mg/20g BW decreased a liver histopathological score but induced a histopathological alteration of the liver at a dose of 1.00 mg/20g BW. Conclusion: This study indicate that EPE could be recommended as a colon anticancer through increase the goblet cells, induce apoptosis through increase TNF-α, and decrease TGF-β.

Exploring the anticancer potential of Eleutherine bulbosa: A systematic network pharmacology study on lung cancer

“…Natural ingredients proven to have anticancer properties include resveratrol. [ 12 13 ] Our findings showed that resveratrol had good bioavailability and interacted with genes associated with lung cancer. Consistently, resveratrol might be involved in the biological process of lung cancer either the P53 signaling pathway (Hsa04115), MicroRNAs in cancer (Hsa05206), and Apoptosis (Hsa04210) either in small-cell lung cancer (Hsa05222) or nonsmall-cell lung cancer (Hsa05223) from network pharmacology followed by PPI network analysis.…”

Functional Plasmon-Activated Water Increases Akkermansia muciniphila Abundance in Gut Microbiota to Ameliorate Inflammatory Bowel Disease

“…After dehydration and mounting, all slides immunostained with IL-6, TNF-α, CK20, or COX-2 antibody were examined and scored by pathologists for the percentage of cells showing specific immunostaining signals. Non-repeatable fields were selected at ×200 magnification to analyze the percentage of positively stained cells (score 1, 0–25%; score 2, 26–50%; score 3, 51–75%; score 4, 76–100%) for the final IHC score [ 70 , 71 ].…”

Organoleptic Analysis, Quality Requirement, And Color Determination of Tilapia Nuggets with Eleutherine palmifolia Extract Coating

“…Adding Dayak onion extract to food products influences the product's characteristics and antioxidant content. Dayak onions (Eleutherine palmifolia) are one of the plants known to have antioxidant (Febrinda et al, 2014), anti-inflammatory (Paramita & Nuryanto, 2019;Insanu et al, 2014), antibacterial (Harlita et al, 2018;Insanu et al, 2014), anti-hypertension (Rauf et al, 2018), inhibitor of -glucosidase and HIV replication (Insanu et al, 2014), and colon anticancer abilities (Mutiah et al, 2020). Dayak onions belong to the Iridaceae family and have long been used by ethnic Dayaks in Borneo as a traditional medicine for various types of diseases such as breast cancer, high blood pressure (hypertension), diabetes mellitus, cholesterol-lowering, heart disease, and stroke (Kuntorini et al, 2016).…”