Activity-Dependent Autocrine-Paracrine Activation of Neuronal P2Y Receptors

Abstract

Activation of P2Y receptors by released nucleotides subserves important autocrine-paracrine functions in various non

Facilitation of transmitter release from rat sympathetic neurons via presynaptic P2Y1 receptors

“…PC12 cell clones stably expressing the rat P2Y1 receptor linked to the green fluorescent protein (P2Y1-GFP) were generated as described previously; here, cells of clone 8 were used (Moskvina et al, 2003).…”

“…PC12 cells, in contrast to SCG neurons, do not express endogenous P2Y1 receptors (Moskvina et al, 2003), but both types of cells do express P2Y12 and P2Y13 receptors (Lechner et al, 2004). To investigate whether P2Y1 receptors might mediate an enhancement of transmitter release in a neuronal background other than SCG neurons, either non-transfected PC12 cells or a PC12 cell clone stably expressing rat P2Y1-GFP (Moskvina et al, 2003) were compared with respect to the modulation of stimulation-evoked noradrenaline release by ADP.…”

“…To investigate whether P2Y1 receptors might mediate an enhancement of transmitter release in a neuronal background other than SCG neurons, either non-transfected PC12 cells or a PC12 cell clone stably expressing rat P2Y1-GFP (Moskvina et al, 2003) were compared with respect to the modulation of stimulation-evoked noradrenaline release by ADP. In agreement with previous results (Lechner et al, 2004), ADP (10 mM) reduced K + -evoked tritium overflow from non-transfected PC12 cells by about 50%; however, when these cells had been treated with pertussis toxin (100 ng·mL -1 for 24 h), ADP failed to cause any significant change ( Figure 5A).…”

“…Recombinant P2Y1 receptors mediate inhibition of currents through Kv7 channels (IM) in PC12 cells (Moskvina et al, 2003). Moreover, ADP and UDP have been found to inhibit IM in rat SCG neurons, but this effect was suggested to involve P2Y6 receptors (Boehm, 1998).…”

“…Heterologously expressed P2Y1 receptors mediate inhibition of Kv7 channels in SCG neurons (Brown et al, 2000) as well as in PC12 cells (Moskvina et al, 2003). Moreover, ADP has been found to inhibit Kv7 channels in SCG neurons, but it was concluded that this effect is mediated by endogenously P < 0.001 P > 0.6…”