Activity-guided isolation and identification of free radical-scavenging components from an aqueous extract of Coleus aromaticus

Kumaran, Alaganandam; Karunakaran, R.

doi:10.1016/j.foodchem.2005.09.051

Cited by 112 publications

(60 citation statements)

References 23 publications

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“…[25] Synthetic antioxidants play an important role in preventing major degenerative diseases caused by free radicals and protecting foodstuffs from lipid oxidation. [26] However, with increasing documentation of possible adverse effects of some synthetic antioxidants on human health, there is an increasing interest in finding natural and biologically produced antioxidants. Further, the emergence of bacterial strains resistant to clinically used antibiotics and changing patterns of susceptibility to clinically available antimicrobial agents require continuous updating of knowledge concerning the treatment of diseases caused by such pathogens.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant, antibacterial, and ultraviolet-protective properties of carotenoids isolated from Micrococcus spp.

Mohana

Thippeswamy

Abhishek

2013

Radiat Prot Environ

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Section: Resultsmentioning

confidence: 99%

Antioxidant, antibacterial, and ultraviolet-protective properties of carotenoids isolated from Micrococcus spp.

Mohana

Thippeswamy

Abhishek

2013

Radiat Prot Environ

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“…Not only for their scavenging properties, the interest in antioxidants has been increasing because of their high capacity in scavenging free radicals related to various diseases (Silva et al, 2007). Numerous studies have shown the strong antioxidant activity and the powerful scavenger activity against free radicals of aromatic or spicy and medicinal plants (Kumaran & Karunakaran, 2007;Vellosa et al, 2006). The use of these plant materials as natural antioxidants for food, cosmetics and other applications becomes necessary not only for food safety issues, but also because they are natural, nonsynthetic products and are more readily acceptable to the consumers.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant characteristics ofDichrostachys glomerataspice extracts Características antioxidantes de los extractos de la especiaDichrostachys glomerata

Kuate

Etoundi

Soukontoua

et al. 2010

CyTA - Journal of Food

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The antioxidant activity of aqueous extract, ethanol extract, and hydroethanolic pod extract of a Cameroonian spice Dichrostachys glomerata (D. glomerata) was investigated. When compared with the two other extracts, the aqueous extract exhibited the lowest phenolic content, AE ABTS (Antiradical Efficiency) and AE DPPH values whereas the ethanol extract had the highest phenolic content, AE ABTS and AE DPPH values. The DPPH (a, a-diphenyl-bpicrylhydrazyl) radical and ABTS (2, 2 0 -azinobis (3-ethylbenzothiazoline-6-sulfonic acid) cation radical scavenging activities were proven and correlated with the reductive potential and phenolic content of the extracts with r 2 greater than 0.9. All extracts had effective, superoxide anion radical, hydroxyl radical and nitric oxide scavenging activity, at all tested concentrations in a concentration-dependent manner. Each extract showed a concentration-dependent effect on chelating activity and a-linoleic acid oxidation inhibition activity. When compared with the controls, each extract significantly decreased malondialdehyde and lipid hydroperoxides formation in low-density lipoprotein (LDL). The hydroethanolic extract exhibited the highest inhibition of LDL oxidation. These results suggest that pods from D. glomerata can be good source of natural antioxidants.Keywords: polyphenols; low-density lipoprotein oxidation; spice; free radical scavenging activity Se estudio´la actividad antioxidante de los extractos acuoso, etano´lico e hidroetano´lico de vaina de una especia camerunense Dichrostacrys glomerata (D. glomerata). Comparado con los otros dos, el extracto acuoso mostro´el contenido en feno´licos ma´s bajo, valores AE ABTS (Eficiencia de Antirradical) y AE DPPH , mientras el extracto de etanol tuvo el contenido en feno´licos ma´s alto, valores AE ABTS y AE DPPH . Los poderes de captacio´n del radical DPPH (a, a-diphenyl-b-picrylhydrazyl) y del catio´n radical ABTS (2, 2 0 -azinobis (3-ethylbenzothiazoline-6-sulfonic acid) fueron probados y correlacionados con el potencial reductivo y contenido en feno´lico de los extractos con r 2 mayor de 0.9. Todos los extractos tuvieron eficacia, poder de captacio´n de radical anio´n supero´xido, radical hidroxilo y o´xido nı´trico, en todas las concentraciones probadas en una manera de dependiente de la concentracio´n. Cada extracto mostro´un efecto dependiente de la concentracio´n tanto en la actividad quelante como en la actividad de inhibicio´n de oxidacio´n de a´cido a-linoleico. Comparado con controles, cada extracto redujo significativamente la formacio´n de malondialdehı´do y hipero´xido lı´pido en lipoproteı´na de baja densidad (LDL). El extracto hidroetano´lico mostro´la mayor inhibicio´n de oxidacio´n LDL. Estos resultados sugieren que las vainas de D. glomerata pueden ser una buena fuente de antioxidantes naturales.Palabras clave: polifenoles; oxidacio´n de lipoproteı´na de baja densidad; especia; poder de captacio´n de radical libre

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“…Chlorogenic acid,rosmarinic acid and caffeic acid are the key bioactivecompounds present in C. aromaticus [50]. Not muchresearch has been done to understand the antiviralproperties of these compounds.…”

Section: B)mentioning

confidence: 99%

Antiviral Activity of Selected Indian Medicinal Herbs Against Hepatitis E Virus (Hev) in the Established Porcine Cell Model

Roy¹,

Kanwar²,

Langerholc³

2017

J Res Educ Indian Med - ISSN 0970-7700

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Abstract:Background: It is known that hepatitis E virus (HEV) is zoonotically transmitted to humans and while other forms of hepatitis have been in focus for drug and vaccine development, HEV related mortality in case of pregnant women can reach upto 20%. Methods: HEV proteins, were successfully extracted from 22 pig stool samples and the presence of HEV capsid antigen encoded by the open reading frame 2 (ORF2) was confirmed using dot blot and western blotting in 4 samples. HEV samples were inoculated in pig epithelial cells (CLAB) and pig macrophage (POM2) to develop an in vitro model for observing the cytopathic effects (CPE). The HEV infected cell cultures were subjected to water: methanol (95:5) extracts from Kaempfaria galanga (Galangal), Mimosa pudica (Touch me not), Coleus aromaticus (Doddapatre)and Paederia foetida (Stinkvine).Results:We showed that incubation of 56 μg/mL HEV samples for 3 hours induced characteristic growth patterns in cells until 4 passages. We also found that extracts of K. galanga significantly (p<0.001) increased cell viability in CLAB cells while other extracts showed cytotoxic effects. In the preventive study (treatments of cells with extracts followed by infection of cells with viral proteins), both 10 -2 and 10 -3 dilutions of K. galanga extracts significantly (p<0.001) prevented a decrease in cell viability of CLAB cells after HEV infection. The 10 -2 dilution of C. aromaticus (p<0.01) and 10 -3 dilution of K. galanga was the only effective (p<0.001) extract in case of HEV infected POM2 cells. Conclusion: A new in vitro cell culture model based on CLAB and POM2 cells has been developed to study HEV.We are also the first to report the activity of these medicinal extracts against HEV in a cell culture model. Extracts of K. galanga and P. foetida showed a promising anti-viral activity and the potential to be used as a natural therapy against HEV.

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Activity-guided isolation and identification of free radical-scavenging components from an aqueous extract of Coleus aromaticus

Cited by 112 publications

References 23 publications

Antioxidant, antibacterial, and ultraviolet-protective properties of carotenoids isolated from Micrococcus spp.

Antioxidant, antibacterial, and ultraviolet-protective properties of carotenoids isolated from Micrococcus spp.

Antioxidant characteristics ofDichrostachys glomerataspice extracts Características antioxidantes de los extractos de la especiaDichrostachys glomerata

Antiviral Activity of Selected Indian Medicinal Herbs Against Hepatitis E Virus (Hev) in the Established Porcine Cell Model

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