Activity-guided isolation and identification of the major antioxidant and anticancer compounds from a commercial Pelargonium sidoides tincture

Pereira, Andreia S.P.; Bester, Megan Jean; Soundy, Puffy; Apostolides, Zeno

doi:10.1007/s00044-015-1425-6

Cited by 9 publications

(9 citation statements)

References 67 publications

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“…The characteristic fragmentation pathways for PACN are the quinone methide mechanism, heterocyclic ring fission, and retro-Diels-Alder (RDA) fission with neutral losses of 168 Da [ 24 ]. These compounds produced m / z ions of 609, 913, 1217, 1521, 1825, respectively, with various fragment ions ( Table 1 ) obtained by the loss of a galloyl and RDA fragments [ 30 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

Efficacy of Proanthocyanidins from Pelargonium sidoides Root Extract in Reducing P. gingivalis Viability While Preserving Oral Commensal S. salivarius

et al. 2018

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Bacterial resistance to antibiotics and the disruption of beneficial microbiota are key problems in contemporary medicine and make the search for new, more efficient infection treatment strategies among the most important tasks in medicine. Multicomponent plant-derived preparations with mild antibacterial activity created by many simultaneous mechanisms together with anti-inflammatory, innate immune and regenerative capacity-stimulating properties are good candidates for this therapy, and proanthocyanidins are among the most promising compounds of this sort. In this study, we have isolated proanthocyanidins from Pelargonium sidoides DC root extract and characterized and compared the composition, antioxidant properties and antibacterial activity of the proanthocyanidin fraction with those of the whole extract. The results revealed that proanthocyanidins had significantly stronger antioxidant capacity compared to the root extract and exhibited a unique antibacterial action profile that selectively targets Gram-negative keystone periodontal and peri-implant pathogenic strains, such as Porphyromonas gingivalis, while preserving the viability of beneficial oral commensal Streptococcus salivarius. The finding suggests that proanthocyanidins from Pelargonium sidoides root extract are good candidates for the prolonged and harmless treatment of infectious diseases.

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Section: Resultsmentioning

confidence: 99%

Efficacy of Proanthocyanidins from Pelargonium sidoides Root Extract in Reducing P. gingivalis Viability While Preserving Oral Commensal S. salivarius

et al. 2018

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“…4 The cell signaling pathways identified here are also under oxidative control as has been observed with the PI3K-Akt and the Janus Kinase (JAK)-signal transducers and activators of transcription protein (STAT) signaling pathways as well as the Src protein kinases that can then go on to activate the PI3K-Akt, Ras/Raf/MAPK and STAT pathways [62][63][64][65][66][67]. The bioactive compounds identified from P. sidoides were also shown to have significant antioxidant activity in vitro [17]. Thus, P. sidoides besides regulating these signal transduction pathways through possible direct interaction with the concerned protein kinases, may also regulate these same pathways by scavenging the reactive oxygen and nitrogen species that can also activate these pathways.…”

Section: Protein Interaction Network and Pathway Enrichment Analysismentioning

confidence: 59%

“…In previous studies, we found anti-proliferative activity for a commercial radix mother tincture of P. sidoides in vitro and through a bioactivity-guided approach; we isolated seven fractions with significant anti-proliferative activity [17,18]. Liquid chromatography with tandem mass spectrometry detection (LC-MS/MS) analysis of these fractions revealed the presence of phenolic compounds gallic acid, koaburaside, proanthocyanidin dimers as well as a trimer, dihydroxycoumarin sulfates, trihydroxycoumarin as well as three turgorins namely leaf movement factor (LMF) 2, LMF3 and the periodic leaf movement factor 1 (PLMF1).…”

Section: Introductionmentioning

confidence: 88%

Exploring the anti-proliferative activity of Pelargonium sidoides DC with in silico target identification and network pharmacology

2017

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Pelargonium sidoides DC (Geraniaceae) is a medicinal plant indigenous to Southern Africa that has been widely evaluated for its use in the treatment of upper respiratory tract infections. In recent studies, the anti-proliferative potential of P. sidoides was shown, and several phenolic compounds were identified as the bioactive compounds. Little, however, is known regarding their anti-proliferative protein targets. In this study, the anti-proliferative mechanisms of P. sidoides through in silico target identification and network pharmacology methodologies were evaluated. The protein targets of the 12 phenolic compounds were identified using the target identification server PharmMapper and the server for predicting Drug Repositioning and Adverse Reactions via the Chemical-Protein Interactome (DRAR-CPI). Protein-protein and protein-pathway interaction networks were subsequently constructed with Cytoscape 3.4.0 to evaluate potential mechanisms of action. A total of 142 potential human target proteins were identified with the in silico target identification servers, and 90 of these were found to be related to cancer. The protein interaction network was constructed from 86 proteins involved in 209 interactions with each other, and two protein clusters were observed. A pathway enrichment analysis identified over 80 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enriched with the protein targets and included several pathways specifically related to cancer as well as various signaling pathways that have been found to be dysregulated in cancer. These results indicate that the anti-proliferative activity of P. sidoides may be multifactorial and arises from the collective regulation of several interconnected cell signaling pathways.

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“…Geranium essential oils are known to exhibit anti-cancer activity [21,22]. Previous studies have shown that the Pelargonium species have cytotoxic activity on cancer cells [2,7,23].…”

Section: Iscussionmentioning

confidence: 99%

“…Although a considerable amount of research has been performed on the biological activities of the Pelargonium species, knowledge on its anticancer activity is limited. Data on this subject suggest that fractions of P. sidoides have anti-growth effects on the Jurkat E6.1 human leukemic T cell lymphoblast cell line [7], while another study reported that P. zonale extract had minimal cytotoxicity against the HeLa cell line [2].…”

Section: Introductionmentioning

confidence: 98%

Total Phenolic Content, Antioxidant and Cyto-/Genotoxic Activities of Pelargonium Quercetorum Agnew in Human Breast Cancer Cells

Arı¹,

Çelikler²,

Karakaş³

et al. 2017

J Clin Exp Invest

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Because the current cancer treatment success rate is not sufficient, plants have been gaining importance as a possible source for anti-cancer compounds. This study aimed to investigate both the genotoxic and cytotoxic activities of methanol extracts of Pelargonium quercetorum Agnew (P. quercetorum), which is traditionally used for its antihelminthic activity, but has not yet been studied for its effect in breast cancer cells. Methods: In this study, the cyto-genotoxic activities of methanol extracts of Pelargonium quercetorum were investigated in human breast cancer cells (MCF-7 and MDA-MB-231). The cytotoxic effect of the extract on these cells was evaluated by MTT and ATP viability assays. The mode of cell death (apoptosis/necrosis) was determined using fluorescence microscopy and biochemical methods. Genotoxic activity was studied with Comet assay. In addition, the total phenolic content and antioxidant capacity were determined by the Folin-Ciocalteu and ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] methods, respectively. Results: Results of this study reveal that Pelargonium quercetorum has a large total phenolic content and high antioxidant capacity. Pelargonium quercetorum induced anti-growth effects in a dose-dependent manner in cancer cells. The extract killed the cells by apoptosis as evidenced by the presence of pyknotic nucleus and annexin V-FITC positivity. The extract also exerted genotoxic activity at relatively low doses. Conclusion: These results suggest that Pelargonium quercetorum induces apoptosis-like cell death by causing DNA damage in breast cancer cells.

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Activity-guided isolation and identification of the major antioxidant and anticancer compounds from a commercial Pelargonium sidoides tincture

Cited by 9 publications

References 67 publications

Efficacy of Proanthocyanidins from Pelargonium sidoides Root Extract in Reducing P. gingivalis Viability While Preserving Oral Commensal S. salivarius

Efficacy of Proanthocyanidins from Pelargonium sidoides Root Extract in Reducing P. gingivalis Viability While Preserving Oral Commensal S. salivarius

Exploring the anti-proliferative activity of Pelargonium sidoides DC with in silico target identification and network pharmacology

Total Phenolic Content, Antioxidant and Cyto-/Genotoxic Activities of Pelargonium Quercetorum Agnew in Human Breast Cancer Cells

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