Activity Increase After Extraction of Alkaline Phosphatase from Human Osteoblastic Membranes by Nonionic Detergents: Influence of Age and Sex

Bourrat, C.; Radisson, J.; Chavassieux, Pascale; Azzar, Gérard; Roux, B.; Meunier, Pierre J.

doi:10.1007/s002230050006

Cited by 5 publications

(4 citation statements)

References 53 publications

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“…Various amounts of cellular components were detectable in the ECM. Particularly, ALP as a representative for membrane proteins17–19 was removed only incompletely. This will be true also for other proteins, like intercellular adhesion molecules and major histocompatibility antigens 37, 38.…”

Section: Discussionmentioning

confidence: 99%

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Native extracellular matrix coating on Ti surfaces

Pham¹,

Reuther²,

Maitz³

2003

J Biomedical Materials Res

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Osteoblast-like SAOS-2 cells were allowed to synthesize and assemble their extracellular matrix (ECM) on titanium surfaces. After the selective removal of cells, Ti coated with a native ECM was obtained (ECM-Ti). The responses of SAOS-2 cells to ECM-Ti compared with those to peptide sequence RGDS- or fibronectin-immobilized Ti were examined, demonstrating the compositional and structural effects needed to trigger the native cell behavior.

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Section: Discussionmentioning

confidence: 99%

“…ALP (EC 3.1.3.1) is the specific enzyme of bone forming cells. It is localized in the cell membrane of osteoblasts17–19 and matrix vesicles 20, 21. The detectable activity of this enzyme indicates remaining membrane components after the NH 4 OH treatment.…”

Section: Methodsmentioning

confidence: 98%

Native extracellular matrix coating on Ti surfaces

Pham¹,

Reuther²,

Maitz³

2003

J Biomedical Materials Res

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“…The proteins were separated using ultra-centrifugation filters with a molecular weight cut-off of 100 kDa (Amicon Ultra; MilliporeSigma). The enzymatic activity was evaluated in the >100 kDa protein fraction as human bone-specific ALP has an approximate weight of 140 kDa (30). The total protein was quantified according to the modified method of Lowry (31).…”

Section: Protein Expression In the Mineralised Matrixmentioning

confidence: 99%

Docosahexaenoic acid improves altered mineralization proteins, the decreased quality of hydroxyapatite crystals and suppresses oxidative stress induced by high glucose

et al. 2022

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Patients with type 2 diabetes mellitus (DM2) experience an increased risk of fractures and a variety of bone pathologies, such as osteoporosis. The suggested mechanisms of increased fracture risk in DM2 include chronic hyperglycaemia, which provokes oxidative stress, alters bone matrix, and decreases the quality of hydroxyapatite crystals. Docosahexaenoic acid (DHA), an omega-3 fatty acid, can increase bone formation, reduce bone loss, and it possesses antioxidant/anti-inflammatory properties. The present study aimed to determine the effect of DHA on altered osteoblast mineralisation and increased reactive oxygen species (ROS) induced by high glucose concentrations. A human osteoblast cell line was treated with 5.5 mM glucose (NG) or 24 mM glucose (HG), alone or in combination with 10 or 20 µM DHA. The collagen type 1 (Col1) scaffold, the expression of osteocalcin (OCN) and bone sialoprotein type-II (BSP-II), the alkaline phosphatase (ALP) specific activity, the mineral quality, the production of ROS and the mRNA expression of nuclear factor erythroid 2-related factor-2 (NRF2) were analysed. Osteoblasts cultured in HG and treated with either DHA concentration displayed an improved distribution of the Col1 scaffold, increased OCN and BSP-II expression, increased NRF2 mRNA, decreased ALP activity, carbonate substitution and reduced ROS production compared with osteoblasts cultured in HG alone. DHA counteracts the adverse effects of HG on bone mineral matrix quality and reduces oxidative stress, possibly by increasing the expression of NRF2.

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“…the different solubilization of TNAP from osteoblast plasma membrane, obtained from human primary bone cell culture, it was suggested that changes in TNAP activity result from age-related modifications. These changes could be associated with the posttranslational modification of TNAP or with the membrane constituents (Radisson et al, 1996;Bourrat et al, 2000). The role of TNAP in mineral formation was evidenced in the case of hypophosphatasia, an inheritable disorder leading to a defective bone formation and characterized by a deficiency in TNAP (Whyte, 1994).…”

Section: Alkaline Phosphatase and Related Proteins In The Matrix Vesimentioning

confidence: 99%

The roles of annexins and alkaline phosphatase in mineralization process.

Balcerzak

Hamade²,

Zhang³

et al. 2003

Acta Biochim Pol

182

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In this review the roles of specific proteins during the first step of mineralization and nucleation are discussed. Mineralization is initiated inside the extracellular organelles-matrix vesicles (MVs). MVs, containing relatively high concentrations of Ca2+ and inorganic phosphate (Pi), create an optimal environment to induce the formation of hydroxyapatite (HA). Special attention is given to two families of proteins present in MVs, annexins (AnxAs) and tissue-nonspecific alkaline phosphatases (TNAPs). Both families participate in the formation of HA crystals. AnxAs are Ca2+ - and lipid-binding proteins, which are involved in Ca2+ homeostasis in bone cells and in extracellular MVs. AnxAs form calcium ion channels within the membrane of MVs. Although the mechanisms of ion channel formation by AnxAs are not well understood, evidence is provided that acidic pH or GTP contribute to this process. Furthermore, low molecular mass ligands, as vitamin A derivatives, can modulate the activity of MVs by interacting with AnxAs and affecting their expression. AnxAs and other anionic proteins are also involved in the crystal nucleation. The second family of proteins, TNAPs, is associated with Pi homeostasis, and can hydrolyse a variety of phosphate compounds. ATP is released in the extracellular matrix, where it can be hydrolyzed by TNAPs, ATP hydrolases and nucleoside triphosphate (NTP) pyrophosphohydrolases. However, TNAP is probably not responsible for ATP-dependent Ca2+/phosphate complex formation. It can hydrolyse pyrophosphate (PPi), a known inhibitor of HA formation and a byproduct of NTP pyrophosphohydrolases. In this respect, antagonistic activities of TNAPs and NTP pyrophosphohydrolases can regulate the mineralization process.

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Activity Increase After Extraction of Alkaline Phosphatase from Human Osteoblastic Membranes by Nonionic Detergents: Influence of Age and Sex

Cited by 5 publications

References 53 publications

Native extracellular matrix coating on Ti surfaces

Native extracellular matrix coating on Ti surfaces

Docosahexaenoic acid improves altered mineralization proteins, the decreased quality of hydroxyapatite crystals and suppresses oxidative stress induced by high glucose

The roles of annexins and alkaline phosphatase in mineralization process.

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