Activity of 3 -hydroxysteroid dehydrogenase in the postovulatory follicle of the domestic fowl (Gallus domesticus)

Armstrong, David G.; Davidson, Maida F.; Gilbert, A. B.; Wells, John W.

doi:10.1530/jrf.0.0490253

Cited by 25 publications

(14 citation statements)

References 11 publications

(11 reference statements)

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“…The decline in progesterone content of the granulosa and thecal fractions of the POF during the first 15 h after ovulation is similar to that observed for 3ß-HSD activity in this tissue (Armstrong et al, 1977). The tissue content of other steroid hormones showed erratic patterns but there were transient increases in oestrogens in the POF between 20 and 27 h after ovulation.…”

Section: Discussionsupporting

confidence: 77%

“…Husbandry conditions, egg readings, collection and histology of tissue fractions of the POF were as described by Armstrong et al (1977). A further 5 hens were selected 1 h before an expected ovulation (see Gilbert et al, 1978).…”

Section: Animalsmentioning

confidence: 99%

“…In addition, removal or even damage of the unicellular layer of granulosa cells of the POF leads to delayed oviposition (Gilbert, Davidson & Wells, 1978). The enzyme 3ß-hydroxysteroid dehydrogenase (3ß-HSD) has been demonstrated in the granulosa and theca cells of the POF by histochemical and biochemical procedures (Armstrong, Davidson, Gilbert & Wells, 1977), and steroid hormones may therefore be produced by the POF to modulate oviductal function. The present study was an examination of the amounts of five steroid hormones in the granulosa and thecal fractions of POFs collected at known times after ovulation.…”

Section: Introductionmentioning

confidence: 99%

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Steroid hormones in the postovulatory follicle of the domestic fowl (Gallus domesticus)

Dick¹,

Culbert²,

Wells³

et al. 1978

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Summary. Progesterone, androstenedione, testosterone, oestrone and oestradiol were measured in the postovulatory follicle (POF) at various times up to 52 h after ovulation. The 3-fold decrease in progesterone, the major constituent, which occurred over the first 15\p=m-\20h resembled the changes previously described for the enzyme, 3\g=b\-hydroxy\x=req-\ steroid dehydrogenase.At 1 h before ovulation the granulosa cells of the anteovulatory follicle (AOF) contained 50 times more progesterone than the POF granulosa fraction collected 2\p=m-\3h later. The thecal portion of the AOF had progesterone concentrations 5 times those of the POF theca, but the latter contained higher concentrations of androstenedione and oestrone.

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Section: Discussionsupporting

confidence: 77%

Section: Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Steroid hormones in the postovulatory follicle of the domestic fowl (Gallus domesticus)

Dick¹,

Culbert²,

Wells³

et al. 1978

Reproduction

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“…The ability of the postovulatory follicle to bind significant amounts of FSH is somewhat surprising. The postovulatory follicle does not possess any steroidogenic capacity (Armstrong, Davidson, Gilbert & Wells, 1977;Dick, Culbert, Wells, Gilbert & Davidson, 1978) and its function, if any, remains vague. However, a 100-fold increase in prostaglandin synthesis by this tissue has been reported by Day & Nalbandov (1977) 1-2 h before oviposition and the changes in FSH binding of the postovulatory follicle may be related to this phenomenon.…”

Section: Resultsmentioning

confidence: 99%

Changes in the Plasma Concentrations of Luteinizing Hormone, Progesterone, Oestradiol and Testosterone and in the Binding of Follicle-Stimulating Hormone to the Theca of Follicles During the Ovulation Cycle of the Hen (Gallus Domesticus)

Etches¹,

Cheng²

1981

Journal of Endocrinology

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The changes in the binding of FSH during follicular maturation were examined in the hen using 125I-labelled bovine FSH (bFSH) and unlabelled bFSH. The binding of 125I-labelled bFSH was not inhibited by bovine LH or chicken LH but was inhibited by extracts of chicken pituitary glands. The ovarian stroma, which contained both interstitial tissue and small follicles, bound the greatest amount of FSH. As the follicles progressed through the yolk-filled hierarchy of maturation, they bound decreasing amounts of FSH. In the two largest follicles of the hierarchy, there was a significant increase in the binding of FSH 12-16h before ovulation. There were two peaks in the concentrations of LH; a preovulatory peak occurred 4-6h before ovulation and a second peak occurred 14-16h before ovulation. Plasma concentrations of testosterone, oestradiol and progesterone began to rise 9, 8 and 6h, respectively, before ovulation. These data are consistent with the hypothesis that changes in the gonadotrophin concentration and binding regulate the order of the follicular hierarchy and the onset of preovulatory steroidogenesis in the hen.

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“…No loss in enzyme activity was observed after 7 days of storage under these conditions. Assays 3ß-Hydroxy-A5-steroid dehydrogenase, isocitrate dehydrogenase (NADP + ) and glucose-6-phosphate dehydrogenase were assayed by the method described by Armstrong, Davidson, Gilbert & Wells (1977) and Bergmeyer (1974a, b) respectively. Protein was measured by the method of Lowry, Rosebrough, Farr & Randall (1951).…”

Section: Animalsmentioning

confidence: 99%

3β-Hydroxy-Δ5-steroid dehydrogenase activity in the rapidly growing ovarian follicles of the domestic fowl (Gallus domesticus)

Armstrong¹

1982

Journal of Endocrinology

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The total and specific activity of 3 beta-hydroxy-delta 5-steroid dehydrogenase, isocitrate dehydrogenase (NADP+) and glucose-6-phosphate dehydrogenase were measured in ovarian follicles from the domestic fowl. The enzymes were assayed in the five largest yolk-filled follicles which were sampled twice during the ovulatory cycle, at 1 h and 16 h before an expected ovulation. The total and specific activity of granulosa enzymes increased throughout the hierarchy and reached a maximum in the largest follicle. The relative increase in 3 beta-hydroxy-delta 5-steroid dehydrogenase activity was greater than that of the other two enzymes examined. The total thecal 3 beta-hydroxy-delta 5-steroid dehydrogenase activity reached a maximum in the third and fourth largest follicles. Thereafter its activity decreased up to the time of ovulation. The activity of 3 beta-hydroxy-delta 5-steroid dehydrogenase and glucose-6-phosphate dehydrogenase in follicles collected 1 h before and ovulation were significantly less than the activity in corresponding follicles collected 16 h before an ovulation.

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Activity of 3 -hydroxysteroid dehydrogenase in the postovulatory follicle of the domestic fowl (Gallus domesticus)

Abstract: Summary. The activity of The results appear consistent with the hypothesis that the POF is a source of steroid hormones.

Cited by 25 publications

References 11 publications

Steroid hormones in the postovulatory follicle of the domestic fowl (Gallus domesticus)

Steroid hormones in the postovulatory follicle of the domestic fowl (Gallus domesticus)

Changes in the Plasma Concentrations of Luteinizing Hormone, Progesterone, Oestradiol and Testosterone and in the Binding of Follicle-Stimulating Hormone to the Theca of Follicles During the Ovulation Cycle of the Hen (Gallus Domesticus)

3β-Hydroxy-Δ5-steroid dehydrogenase activity in the rapidly growing ovarian follicles of the domestic fowl (Gallus domesticus)

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