Activity of a nitrofurazone matrix urinary catheter against catheter-associated uropathogens

Johnson, James R.; Berggren, Terriel; Conway, Ann J.

doi:10.1128/aac.37.9.2033

Cited by 47 publications

(33 citation statements)

References 27 publications

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“…Furthermore, this combination was superior to nitrofurazone coating in inhibiting P. aeruginosa adherence to catheters (Table 2). This is in agreement with the findings of Johnson et al (19,20) that both silver hydrogel and nitrofurazone coatings are not effective against P. aeruginosa. The adhesion assay results were further confirmed by CSLM.…”

Section: Discussionsupporting

confidence: 83%

Antibiofilm Activity of GlmU Enzyme Inhibitors against Catheter-Associated Uropathogens

Burton

Gawande

Yakandawala

et al. 2006

Antimicrob Agents Chemother

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The colonization of uropathogenic bacteria on urinary catheters resulting in biofilm formation frequently leads to the infection of surrounding tissue and often requires removal of the catheter. Infections associated with biofilms are difficult to treat since they may be more than 1,000 times more resistant to antibiotics than their planktonic counterparts. We have developed an antibiofilm composition comprising an N-acetyl-Dglucosamine-1-phosphate acetyltransferase (GlmU) inhibitor and protamine sulfate, a cationic polypeptide. The antibiofilm activity of GlmU inhibitors, such as iodoacetamide (IDA), N-ethyl maleimide (NEM), and NEM analogs, including N-phenyl maleimide, N,N-(1,2-phenylene)dimaleimide (oPDM), and N-(1-pyrenyl)maleimide (PyrM), was tested against that of catheter-associated uropathogens. Both IDA and NEM inhibited biofilm formation in Escherichia coli. All NEM analogs showed antibiofilm activity against clinical isolates of E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus epidermidis, and Enterococcus faecalis. The combination of oPDM with protamine sulfate (PS) enhanced its antibiofilm activity and reduced its effective concentration to as low as 12.5 M. In addition, we found that the in vitro inhibitory activity of oPDM-plus-PS-coated silicone catheters against P. aeruginosa and S. epidermidis colonization was superior to that of catheters coated with silver hydrogel. Confocal scanning laser microscopy further confirmed that the oPDMplus-PS-coated silicone catheters were almost free from bacterial colonization. Thus, a broad-spectrum antibiofilm composition comprising a GlmU inhibitor and protamine sulfate shows promise for use in antiinfective coatings for medical devices, including urinary catheters.Microorganisms can attach to and colonize any biomaterial surface, putting patients at risk for local and systemic infections. More than 900,000 episodes of catheter-associated urinary tract infections occur annually in acute-care hospitals in the United States, accounting for 40% of all nosocomial infections and involving between 10 and 30% of patients with indwelling urinary catheters (30). Catheter-associated urinary tract infection prolongs the hospital stay between an estimated 2.4 and 4.5 days, with resultant increased healthcare costs (15,16). Recent studies have shown that a wide range of persistent catheter-related infections may be related to the ability of bacteria to form biofilms (6, 28). Treatment of device-related infections with conventional antimicrobial agents frequently fails because microorganisms growing in biofilms are more tolerant or phenotypically resistant to antimicrobial agents than planktonic cells (24). The insensitivity of biofilm bacteria to antibiotics is a function of cell wall composition, surface structure, and phenotypic variation in enzymatic activity (8,14). It has also been suggested that the negatively charged exopolysaccharide is very effective in protecting bacterial cells from cationic antibiotics by restricting their permeation (2...

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Section: Discussionsupporting

confidence: 83%

Antibiofilm Activity of GlmU Enzyme Inhibitors against Catheter-Associated Uropathogens

Burton

Gawande

Yakandawala

et al. 2006

Antimicrob Agents Chemother

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“…Many efforts have been made to overcome the infectious complications associated with indwelling urological devices, primarily by modification of the catheter biomaterial by, for example, impregnation or coating with conventional antimicrobials or silver (Johnson et al, 1993;Ahearn et al, 2000). However, in clinical practice, these approaches have not proved as successful as would have been desired (Lee et al, 2004;Johnson et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

The use of lytic bacteriophages in the prevention and eradication of biofilms ofProteus mirabilisandEscherichia coli

Carson

Gorman

Gilmore

2010

FEMS Immunol Med Microbiol

152

123

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Antibiotics have been the cornerstone of the clinical management of bacterial infections since their discovery in the early part of the last century. Eight decades later, their widespread, often indiscriminate use, has resulted in an overall reduction in their effectiveness, with reports of multidrug-resistant bacteria now commonplace. Increasing reliance on indwelling medical devices, which are inherently susceptible to biofilm-mediated infections, has contributed to unacceptably high rates of nosocomial infections, placing a strain on healthcare budgets. This study investigates the use of lytic bacteriophages in the treatment and prevention of biofilms of bacterial species commonly associated with infections of indwelling urological devices and catheter-associated urinary tract infections. The use of lytic bacteriophages against established biofilms of Proteus mirabilis and Escherichia coli is described, whereby biofilm populations have been reduced successfully by three to four log cycles (99.9-99.99% removal). The prevention of biofilm formation on Foley catheter biomaterials following impregnation of hydrogel-coated catheter sections with a lytic bacteriophage has also been investigated. This has revealed an approximate 90% reduction in both P. mirabilis and E. coli biofilm formation on bacteriophage-treated catheters when compared with untreated controls.

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“…Such materials or molecules may be coated on catheter surfaces or incorporated into matrices from which they are released in a continuous manner into the nearsurface environment. Specifically, the release of silver (Desai et al, 2010;Johnson et al, 2010) and various antibiotics such as triclosan (Bayston et al, 2009), nitrofurazone (Desai et al, 2010;Johnson et al, 1993Johnson et al, , 2010, and the combination of minocycline and rifampicin (Raad et al, 2008) from catheter surfaces has shown some promise. However, such approaches generally have limited utility due to restrictions associated with the amount and duration over which the biocidal agent can be released.…”

Section: Introductionmentioning

confidence: 98%

Acylase‐containing polyurethane coatings with anti‐biofilm activity

Grover

Plaks

Summers

et al. 2016

Biotech & Bioengineering

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Due to the prevalence of biofilm-related infections, which are mediated by bacterial quorum sensing, there is a critical need for materials and coatings that resist biofilm formation. We have developed novel anti-biofilm coatings that disrupt quorum sensing in surface-associated bacteria via the immobilization of acylase in polyurethane films. Specifically, acylase from Aspergillus melleus was covalently immobilized in biomedical grade polyurethane coatings via multipoint covalent immobilization. Coatings containing acylase were enzymatically active and catalyzed the hydrolysis of the quorum sensing (QS) molecules N-butyryl-L-homoserine lactone (C4-LHL), N-hexanoyl-L-homoserine lactone (C6-LHL), and N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-LHL). In biofilm inhibition assays, immobilization of acylase led to an approximately 60% reduction in biofilm formation by Pseudomonas aeruginosa ATCC 10145 and PAO1. Inhibition of biofilm formation was consistent with a reduction in the secretion of pyocyanin, indicating the disruption of quorum sensing as the mechanism of the coating activity. Scanning electron microscopy further showed that acylase-containing coatings contained far fewer bacterial cells than control coatings that lacked acylase. Moreover, acylase-containing coatings retained 90% activity when stored dry at 37°C for 7 days and were more stable than the free enzyme in physiological conditions, including artificial urine. Ultimately, such coatings hold considerable promise for the clinical management of catheter-related infections as well as the prevention of infections in orthopedic applications (i.e., on hip and knee prostheses) and on contact lenses. Biotechnol. Bioeng. 2016;113: 2535-2543. © 2016 Wiley Periodicals, Inc.

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Activity of a nitrofurazone matrix urinary catheter against catheter-associated uropathogens

Cited by 47 publications

References 27 publications

Antibiofilm Activity of GlmU Enzyme Inhibitors against Catheter-Associated Uropathogens

Antibiofilm Activity of GlmU Enzyme Inhibitors against Catheter-Associated Uropathogens

The use of lytic bacteriophages in the prevention and eradication of biofilms ofProteus mirabilisandEscherichia coli

Acylase‐containing polyurethane coatings with anti‐biofilm activity

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