2020
DOI: 10.1128/aac.02204-19
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Activity of Antibiotics against Pseudomonas aeruginosa in an In Vitro Model of Biofilms in the Context of Cystic Fibrosis: Influence of the Culture Medium

Abstract: Pseudomonas aeruginosa is a major cause of respiratory biofilm-related infections in patients with cystic fibrosis. We developed an in vitro pharmacodynamic model to study the activity of antipseudomonal antibiotics against PAO1 biofilms grown in artificial sputum medium with agar [ASM(+)] versus that against biofilms grown in Trypticase soy broth supplemented with glucose and NaCl (TGN). We measured bacterial counts, metabolic activity (fluorescein diacetate [FDA] hydrolysis), and biomass (crystal violet abso… Show more

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Cited by 22 publications
(21 citation statements)
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“…Remarkably, B-MMIC of tobramycin in aerobiosis was similar in all tested media except in ASM in which lower activity was detected. This is in accordance with a very recently published study in which this antimicrobial agent was more active in the control medium (Trypticase soy broth supplemented with glucose and NaCl) than in ASM [ 11 ].…”
Section: Resultssupporting
confidence: 93%
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“…Remarkably, B-MMIC of tobramycin in aerobiosis was similar in all tested media except in ASM in which lower activity was detected. This is in accordance with a very recently published study in which this antimicrobial agent was more active in the control medium (Trypticase soy broth supplemented with glucose and NaCl) than in ASM [ 11 ].…”
Section: Resultssupporting
confidence: 93%
“…Azithromycin also showed higher P-MMIC values in Synthetic Cystic Fibrosis Medium (SCFM) and in a modification of SCFM (SCFM-2) when microaerobic conditions were used (≥2 dilutions) ( Table 1 ). Differences in P-MMIC of some antimicrobials (such as tobramycin, ciprofloxacin, ceftazidime, and meropenem in P. aeruginosa or vancomycin, azithromycin, tobramycin, and linezolid in Staphylococcus aureus ), have been previously described comparing cation-adjusted Mueller-Hinton broth (CAMHB) medium with other media [ 11 , 16 ]. However, while in our study P-MMIC to tobramycin was higher in ASM medium (4 mg/L) than in CAMHB (0.5 mg/L), in the study of Díaz-Iglesias et al, similar MIC values were obtained in both media (0.125 and 0.25 mg/L, in ASM and CAMHB medium, respectively) [ 11 ].…”
Section: Resultsmentioning
confidence: 99%
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