Activity of Biosynthesized Reduced Graphene Oxide against Multidrug resistant Uropathogenic Bacteria

Abstract

The effect of Bacterially Reduced Graphene Oxide (BRGO) as inhibitory agent alone and in combination with antibiotics against some multidrug resistant (MDR) uropathogenic bacterial isolates as well as the antibiofilm activity was investigated. BRGO nanosheets were synthesized biologically by Escherichia coli strain E-NO.7 (accession no. MK685205). Different concentrations 0.1, 0.5, 1 and 10 mg/ml of BRGO nanosheets showed potent inhibitory effects of all concentrations against tested MDR uropathogenic Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, and Methicillin-Resistant S. aureus (MRSA) isolates. Results showed that the growth inhibition zones increased with increasing in BRGO concentration. The combinations of BRGO (0.5 mg/ml) and different broad spectrum antibiotics exhibited enhanced antibacterial activity against all the studied isolates in comparison with the effects of antibiotics alone. It has been observed that BRGO effectively restricted biofilm formation, and the antibiofilm effect was dose-dependent, since the biofilm inhibition gradually increased with increasing in BRGO concentration.