Activity of cholinesterases and adenosine deaminase in blood and serum of rats experimentally infected withTrypanosoma cruzi

Abstract

This study aimed to evaluate the activity of cholinesterases and adenosine deaminase (ADA) in blood and serum of rats infected with Trypanosoma cruzi. Twelve adult rats were used in the experiment divided into two uniform groups. Rodents from group A (control group) were non-infected and animals from group B served as infected, receiving intraperitoneally 3.3610 7 trypomastigotes/each. Blood collection was performed at days 60 and 120 post-infection (PI) in order to evaluate the hemogram, blood activity of acetylcholinesterase, and serum butyrylcholinesterase and ADA activities. Hematological parameters did not differ between groups. A significant increase (P,0.05) of acetylcholinesterase activity was observed in blood while butyrylcholinesterase had a significant reduction (P,0.01) in serum of infected rats at days 60 and 120 PI. ADA activity in serum showed an inhibition in infected animals when compared to non-infected at day 120 PI. Based on these results, it is possible to conclude that the activity of cholinesterases and ADA were changed in animals infected with T. cruzi. The possible causes of these alterations will be discussed in this paper.

Demodex canis regulates cholinergic system mediated immunosuppressive pathways in canine demodicosis

“…Apparently, no scientific reports demonstrating the association between cholinergic immunosuppressive pathway and mites-induced dermatological conditions in animals and human patients are available. However, our observations in the present study are in agreement with the previous scientific reports demonstrating the role of the cholinergic anti-inflammatory pathways in maintenance and progression of other parasitic diseases in dogs and experimental animals (Da Silva et al 2011; Wolkmer et al . 2013; D'Elia Zanella et al .…”

Purinergic system ecto-enzymes participate in the thromboregulation of patients with indeterminate form of Chagas disease

“…The reduction in E-ADA activity observed in platelets of IFCD patients was also observed in serum of rats experimentally infected by T. cruzi, on day 120 post-infection, associated to absence of parasitemia and clinical and hematological changes, and presence of mononuclear inflammatory infiltrate in heart tissue [49]. This enzymatic reduction would lead to increase in the extracellular adenosine levels and consequently inhibition of the pro-inflammatory effects, attenuating inflammation and tissue damage caused by parasite, since persistent presence of T. cruzi in the cardiac muscle was detected by polymerase chain reaction.…”

Stigma, participation restriction and mental distress in patients affected by leprosy, cutaneous leishmaniasis and Chagas disease: a pilot study in two co-endemic regions of eastern Colombia

“…In a 2010 review, da Silva et al found that chronic CD leads to sleep dysfunction and memory impairment in rats. 27 Furthermore, this review provides evidence of memory impairment, quality of life reduction and depression, as well as direct central nervous system effects in infected humans. However, national programs and operational research still focus primarily on vector control, neglecting the potential psychosocial aspects of CD.…”