Activity of Corilagin on Post-Parasiticide Liver Fibrosis in Schistosomiasis Animal Model

Abstract

The first two authors contributed equally to this study This study investigates the effects and possible molecular mechanisms of corilagin extraction on prevention of Schistosoma japonicum ova-induced granulomas and liver fibrosis. As a result, under a light microscope, when compared to a model group, the corilagin group showed smaller granulomas, less liver cell denaturation and less inOammatory cell infiltration, and the connective tissues were significantly decreased. By Masson staining, the liver sections from the corilagin group showed less collagen distributed around granulomas, decreased liver fibrosis in the portal tracts and less formed interlobular tissue. The expression of hydroxyproline, IL-13 in liver and GATA3 in spleen in the model group was significantly higher than that in the normal group (P<0.05 or 0.01), while the level of hydroxyproline, IL-13 and GATA3 in the corilagin group were significantly lower than that in the model group (P<0.05). In conclusion, corilagin extraction can decrease the level of Th2-associated profibrotic cytokine IL-13, and down-regulate the transcription of GATA3 mRNA in spleen cells, which alleviate the hepatic fibrosis caused by egg granuloma in Schistosoma japonicum infection.Schistosomiasis is a chronic, sometimes fatal, disease with serious symptoms including liver fibrosis, ascites and splenomegaly. The main pathogen is the helminth parasite Schistosoma and the adult

Anti-inflammatory and anti-oxidative effects of corilagin in a rat model of acute cholestasis

“…Furthermore, it was confirmed that corilagin is an inhibitor of TNF-α [16] and can restrain radiation-induced microglia activation via suppression of the NF-κB pathway [17], and corilagin is protective against GalN/LPS-induced liver injury through suppression of oxidative stress and apoptosis [18]. Our recent research showed that corilagin can alleviate the hepatic fibrosis caused by egg granuloma in Schistosoma japonicum infection [19]. …”

Corilagin controls post-parasiticide schistosome egg-induced liver fibrosis by inhibiting Stat6 signalling pathway

“…Some of our previous studies on Corilagin have confirmed its effects on ameliorating sepsis (21) as well as its anti-oxidative and anti-inflammatory effects (29) and suppressive effects on HSV-1 encephalitis (30). Moreover, our studies have demonstrated that IL-13 is associated with the multi-target effects of Corilagin on relieving schistosome egg-induced liver fibrosis (12, 32, 33). On the basis of the above findings, further study of Corilagin is warranted.…”

“…Fibrosis was scored as 5 grades according to the METAVIR scoring system (51, 52): F0, no fibrosis; F1, portal fibrosis without septa; F2, portal fibrosis with few septa; F3, numerous septa without cirrhosis; and F4, cirrhosis. The degree of hepatic fibrosis was detected to elevate the score F0(2 0 )=1, F1(2 1 )=2, F2(2 2 )=4, and F3(2 3 )=8 (12). The mRNA levels of Alpha α-SMA further demonstrated that the degree of hepatic fibrosis from the Corilagin group was significantly alleviated (P<0.05).…”

Effects of corilagin on alleviating cholestasis via farnesoid X receptor‐associated pathways in vitro and in vivo

“…Following our previous protocols (Huang et al, ; Zhou et al, ), total RNA from liver tissues and cells was isolated using RNAiso Plus following the manufacturer's instructions. cDNAs were produced using a PrimeScript RT reagent kit and incubated at 37°C for 15 min and 85°C for 5 s. Real‐time PCR reactions were performed using a StepOne Plus device (Applied Biosystems) at 95°C for 10 s followed by 40 cycles of 95°C for 5 s and 60°C for 20 s according to the instructions provided by the SYBR Premix Ex Taq kit.…”