Activity of Daptomycin against
            <i>Listeria monocytogenes</i>
            Isolates from Cerebrospinal Fluid

Spanjaard, Lodewijk; Vandenbroucke-Grauls, Christina M. J. E.

doi:10.1128/aac.01139-07

Cited by 20 publications

(9 citation statements)

References 14 publications

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“…The lipopeptide antibiotic daptomycin is a frequently used treatment option for gram-positive microorganisms. However, a high daptomycin MIC, as reported previously, was observed in all three isolates [25][26]. Daptomycin resistance has already been described in Staphylococcus spp.…”

Section: Discussionsupporting

confidence: 82%

Genetic structure characteristics and treatment for Listeria monocytogenes infections

Huang

Guo

et al. 2020

Preprint

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Background: Invasive Listeria monocytogenes (Lm) carry a high mortality despite antibiotic treatment. The aim of this study was to investigate the mechanism of pathogenicity and resistance. In addition, the effect of existing treatment options against Lm were systematically evaluated as well. Methods: Three Lm isolates were collected and 15 antibiotics susceptibility tests were done. Subsequently, whole genome sequencing and bioinformatics analysis were performed. Furthermore, the effect of meropenem, linezolid, benzylpenicillin, vancomycin, trimethoprim/sulfamethoxazole were determined using the time-kill assay.Results: Two sequence types (STs) were identified for isolate 23949 (ST87), 26530 (ST3), 34096 (ST87), respectively. All isolates were resistant to fosfomycin and daptomycin. The resistant genes fosX, mprF, norB and vgaALC were identified in all isolates. Furthermore, 80 virulence genes were detected and 72 genes were found in all three isolates. There were 26 virulent genes associated with the structure, biosynthesis, motor switch of flagellum. And other virulent genes were involved in chemotaxis, protease, internalin and metabolism. It is of note that 8 genes (inlJ, llsB, llsD, llsG, llsH, llsP, llsX, llsY) were only found in 26530 isolated from cerebrospinal fluid (CSF), 7 of which were associated with haemolysin. Further in vitro time-kill assay found trimethoprim/sulfamethoxazole at serum or CSF concentrations had bactericidal effect (>3.5 log10 CFU/ml) against three tested Lm strains at 24 h. Conclusions: The involved virulence factors were mainly associated with bacterial pathogenicity. Notably, trimethoprim/sulfamethoxazole might be greater potential therapeutic option against Lm bloodstream infection or intracranial infection.

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Section: Discussionsupporting

confidence: 82%

Genetic structure characteristics and treatment for Listeria monocytogenes infections

Huang

Guo

et al. 2020

Preprint

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“…Although few studies have tested Listeria for susceptibility to daptomycin, a high daptomycin MIC (4 mg/L) has been reported in clinical isolates of L. monocytogenes. Nonetheless, the mechanism of this resistance has not been elucidated [33]. These results could indicate an intrinsic resistance of Listeria spp.…”

Section: Discussionmentioning

confidence: 61%

Characterization of antibiotic resistance in Listeria spp. isolated from slaughterhouse environments, pork and human infections

Moreno

Paixão

Gobbi

et al. 2014

J Infect Dev Ctries

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Introduction: Listeria species are susceptible to most antibiotics. However, over the last decade, increasing reports of multidrug-resistant Listeria spp. from various sources have prompted public health concerns. The objective of this study was to characterize the antibiotic susceptibility of Listeria spp. and the genetic mechanisms that confer resistance. Methodology: Forty-six Listeria spp. isolates were studied, and their minimal inhibitory concentrations of antibiotics were determined by microdilution using Sensititre standard susceptibility MIC plates. The isolates were screened for the presence of gyrA, parC, lde, lsa(A), lnu(A), and mprF by PCR, and the amplified genes were sequenced. Results: All isolates were susceptible to penicillin, ampicillin, tetracycline, erythromycin, and carbapenems. Resistance to clindamycin, daptomycin, and oxacillin was found among L. monocytogenes and L. innocua, and all species possessed at least intermediate resistance to fluoroquinolones. GyrA, parC, and mprF were detected in all isolates; however, mutations were found only in gyrA sequences. A high daptomycin MIC, as reported previously, was observed, suggesting an intrinsic resistance of Listeria spp. to daptomycin. Conclusions: These results are consistent with reports of emerging resistance in Listeria spp. and emphasize the need for further genotypic characterization of antibiotic resistance in this genus.

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“…In vitro MICs to Listeria monocytogenes are elevated compared to those to other Gram-positive bacteria, with an MIC 90 of 4 g/ml (64,65). The L. monocytogenes cell membrane is rich in branched fatty acids (66,67), which may prohibit optimal insertion and oligomerization of daptomycin in the cell membrane, but this has not been specifically demonstrated.…”

Section: Daptomycin Activity Against Gram-positive Bacteriamentioning

confidence: 99%

A Current Perspective on Daptomycin for the Clinical Microbiologist

2013

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SUMMARY Daptomycin is a lipopeptide antimicrobial with in vitro bactericidal activity against Gram-positive bacteria that was first approved for clinical use in 2004 in the United States. Since this time, significant data have emerged regarding the use of daptomycin for the treatment of serious infections, such as bacteremia and endocarditis, caused by Gram-positive pathogens. However, there are also increasing reports of daptomycin nonsusceptibility, in Staphylococcus aureus and, in particular, Enterococcus faecium and Enterococcus faecalis . Such nonsusceptibility is largely in the context of prolonged treatment courses and infections with high bacterial burdens, but it may occur in the absence of prior daptomycin exposure. Nonsusceptibility in both S. aureus and Enterococcus is mediated by adaptations to cell wall homeostasis and membrane phospholipid metabolism. This review summarizes the data on daptomycin, including daptomycin's unique mode of action and spectrum of activity and mechanisms for nonsusceptibility in key pathogens, including S. aureus , E. faecium , and E. faecalis . The challenges faced by the clinical laboratory in obtaining accurate susceptibility results and reporting daptomycin MICs are also discussed.

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Activity of Daptomycin against Listeria monocytogenes Isolates from Cerebrospinal Fluid

Cited by 20 publications

References 14 publications

Genetic structure characteristics and treatment for Listeria monocytogenes infections

Genetic structure characteristics and treatment for Listeria monocytogenes infections

Characterization of antibiotic resistance in Listeria spp. isolated from slaughterhouse environments, pork and human infections

A Current Perspective on Daptomycin for the Clinical Microbiologist

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