Activity of glucose oxidase functionalized onto magnetic nanoparticles

Abstract

BackgroundMagnetic nanoparticles have been significantly used for coupling with biomolecules, due to their unique properties.MethodsMagnetic nanoparticles were synthesized by thermal co-precipitation of ferric and ferrous chloride using two different base solutions. Glucose oxidase was bound to the particles by direct attachment via carbodiimide activation or by thiophene acetylation of magnetic nanoparticles. Transmission electron microscopy was used to characterize the size and structure of the particles while the binding of glucose oxidase to the particles was confirmed using Fourier transform infrared spectroscopy.ResultsThe direct binding of glucose oxidase via carbodiimide activity was found to be more effective, resulting in bound enzyme efficiencies between 94–100% while thiophene acetylation was 66–72% efficient. Kinetic and stability studies showed that the enzyme activity was more preserved upon binding onto the nanoparticles when subjected to thermal and various pH conditions. The overall activity of glucose oxidase was improved when bound to magnetic nanoparticlesConclusionBinding of enzyme onto magnetic nanoparticles via carbodiimide activation is a very efficient method for developing bioconjugates for biological applications

Silica-encapsulated magnetic nanoparticles: Enzyme immobilization and cytotoxic study

“…A suspension of MNPs was synthesized by co-precipitation of Fe +2 and Fe +3 ions, from appropriate mixture solution of ferrous sulfate and ferric chloride, with ammonia, as described earlier [7,19,20]. A microemulsion containing 7.2 mL of cyclohexane, 1.8 mL of n-hexanol, 1.8 mL of Triton X-100 and adequate aqueous magnetic ferrofluid (Fe 3 O 4 ) was prepared.…”

“…The protein concentration was determined using Bradford method. Bovine serum albumin (BSA) was used as the protein standard [7,19,28,29].…”

“…It can be used to determine the quality of glucose in a serum sample, monitor of remaining glucose in food production, remove oxygen from food packaging, in decoloration process and bleaching of natural fibers and to promote saliva and decrease oral bacteria [7,18]. Thermal stability of GOD-MNPs, effect of pH on enzyme activity, storage stability, and reusability were also investigated and discussed.…”

“…The enzyme immobilization onto a water insoluble carrier is a desired biological method to increase applications of the enzyme on continuous procedures and commercial uses [7]. Immobilization can reduce the required amount of enzyme, enhance stability [8] and provide a way for separation of enzyme from reaction mixture [9][10][11][12].…”

A New Route to the Considerable Enhancement of Glucose Oxidase (GOx) Activity: The Simple Assembly of a Complex from CdTe Quantum Dots and GOx, and Its Glucose Sensing

“…[22] The studies of different enzymes assembled onto magnetite nanoparticle surfaces have also demonstrated the potential of enhancing the enzymatic activity. [23,24] However, the investigation of the enhanced activity, stability, and active temperature range of enzymes is still limited to an initial stage.…”