Activity of Hyaluronidase in Placental Tissues from Cows with and without Retained Fetal Membranes

Kankofer, Marta; Wierciński, J; Fidecki, M.

doi:10.1111/j.1439-0442.1998.tb00836.x

Cited by 13 publications

(12 citation statements)

References 21 publications

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“…The level of GSH depends on the activity of γ‐glutamyl transpeptidase which is responsible for the linking of γ glutamyl residues to aminoacids and small peptides. The activity of this enzyme was altered in cases of retained placenta in comparison to control animals (Kankofer and Maj 1997). This may suggest disturbances in the GSH turnover and an imbalance of antioxidative defence mechanisms against ROS.…”

Section: Discussionmentioning

confidence: 90%

Non-enzymatic Antioxidative Defence Mechanisms Against Reactive Oxygen Species in Bovine-retained and not-retained Placenta: Vitamin C and Glutathione

Kankofer¹

2001

Reprod Domest Anim

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Vitamin C and glutathione (GSH) are water-soluble antioxidants which take part in defence mechanisms against reactive oxygen species (ROS). They may also be involved in processes of releasing/retaining bovine fetal membranes. Hence vitamin C, reduced (GSH) and oxidised (GSSG) glutathione levels were determined in retained and not-retained bovine fetal membranes in order to describe the non-enzymatic antioxidative status. Placental samples were collected immediately after spontaneous delivery or during caesarean section before term and at term, and 6 groups were formed as follows: (A) pre-term caesarean section without retained placenta; (B) pre-term caesarean section with retained placenta; (C) term caesarean section without retained placenta; (D) term caesarean section with retained placenta; (E) spontaneous delivery without retained placenta and (F) spontaneous delivery with retained placenta. Homogenates of maternal and fetal placental tissues were prepared, and vitamin C, GSH and GSSG were measured spectrophotometrically. Vitamin C levels were significantly higher in the maternal part than in the fetal part of the placenta in all groups examined. In retained placenta cases the levels were significantly lower than in control cows, except in pre-term groups. GSH concentrations were significantly higher in placentas without retention than with retention. GSSG levels showed the opposite relationship and were significantly higher in samples with retention of fetal membranes than in controls. Further experiments on antioxidative as well as oxidative status in bovine placenta are necessary.

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Section: Discussionmentioning

confidence: 90%

Non-enzymatic Antioxidative Defence Mechanisms Against Reactive Oxygen Species in Bovine-retained and not-retained Placenta: Vitamin C and Glutathione

Kankofer¹

2001

Reprod Domest Anim

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“…Any alteration in proteoglycan metabolism may lead to placental retention in cows, which is the result of inadequate detachment of foetal membranes (Kankofer, Wierciński, & Fidecki, 1998;Kankofer, Wiercinski, & Zerbe, 2000).…”

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confidence: 99%

Patterns of protein glycosylation in bovine placentomes as a function of gestational age and in retained versus non‐retained placenta

Wawrzykowski

Franczyk

Kankofer

2019

Reprod Domestic Animals

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The formation of placenta at the beginning of pregnancy and its separation at parturition require not only deep remodelling of extracellular matrix, which mainly consists of proteins conjugated with sugar moieties, but also the cooperation with cells from both maternal and foetal parts of placenta. The aim of the study was to compare the patterns of selected conjugated proteins with sugar moieties between pregnant and term placenta as well as between released and retained placenta in cows. Placental samples from healthy pregnant cows (3–5 months of pregnancy) were collected at a slaughterhouse (n = 6), and parturient samples were collected during caesarean section at term and retrospectively divided into retained (n = 6) and released (n = 6). The pattern of selected sugar moieties conjugated with proteins was detected by use of lectin blotting with Phaseolus Vulgaris leucoagglutinin, Maackia Amurensis and Sambucus Nigra (Elderberry). The comparison and analysis of obtained band patterns showed differences between their number, molecular weight and abundance related to the intensity of staining. Samples from 3 to 4 months showed similarities, while at the 5th month, clear differences were visible in all 3 lectins, which were used in this study. Samples from retained/released placenta expressed significant differences in PHA‐L and SNA pattern in the foetal part. Obtained results indicate that the development of placenta related to extracellular matrix and accompanying cells from both sides of placenta shows dynamic changes during pregnancy. Moreover, in the case of animals with the retention of foetal membranes the patterns of proteins conjugated with sugar moieties are altered, suggesting that the changes in extracellular matrix metabolism can be involved in the attachment and detachment of the placenta in cows.

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“…It is necessary to note that in addition to Nagase, there are also hyaluronidase and collagenases present in bovine placenta. Their activities may be closely connected with the action of Nagase (Nimni and Harkness 1988) and are also altered in cases of retained fetal membranes in comparison with control animals (Eiler and Hopkins 1992; Maj and Kankofer 1997; Kankofer et al 1998).…”

Section: Discussionmentioning

confidence: 99%

“…Previous results have reported some changes in the activities of collagenases (Maj and Kankofer 1997) and hyaluronidase (Kankofer et al 1998) in cases of retained fetal membranes in cows.…”

Section: Introductionmentioning

confidence: 98%

Activity of Placental β‐N‐Acetyl‐Glucosaminidase in Cows with and without Retained Fetal Membranes

Kankofer¹

2000

Reprod Domestic Animals

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Contents β‐N‐acetyl‐glucosaminidase (Nagase) is one of the enzymes responsible for the metabolism of proteoglycans. It may take part in cell‐to‐cell adhesion processes and might be involved in creating the attachment between the maternal and fetal parts of the bovine placenta. The aim of the following study was the determination of Nagase activity in bovine placental tissues and the comparison of this activity between animals releasing and retaining fetal membranes. The experiment was performed on animals divided into six groups as follows: A, caesarian section before term without retained fetal membranes (n = 14); B, caesarian section before term with retained fetal membranes (n = 14); C, caesarian section at term without retained fetal membranes (n = 15); D, caesarian section at term with retained fetal membranes (n = 8); E, spontaneous delivery at term without retained fetal membranes (n = 16); F, spontaneous delivery at term with retained fetal membranes (n = 10). The enzyme activity was determined spectrofluorimetrically and expressed in nkat/mg protein. Nagase activity increased during last few days before parturition. The activity in general was lower in the fetal than in the maternal part of placenta (in five out of six) of the examined groups of animals. In the maternal part of term placenta, enzyme activity was statistically significantly higher in cases of retained fetal membranes than in control cows. In the fetal part of term placenta the opposite relationship was observed. The results obtained may indicate the involvement of Nagase in the processes of releasing/retaining bovine fetal membranes. Further experiments are necessary in order to explain the mechanisms and substances responsible for controlling the enzyme activity in the placenta.

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Activity of Hyaluronidase in Placental Tissues from Cows with and without Retained Fetal Membranes

Cited by 13 publications

References 21 publications

Non-enzymatic Antioxidative Defence Mechanisms Against Reactive Oxygen Species in Bovine-retained and not-retained Placenta: Vitamin C and Glutathione

Non-enzymatic Antioxidative Defence Mechanisms Against Reactive Oxygen Species in Bovine-retained and not-retained Placenta: Vitamin C and Glutathione

Patterns of protein glycosylation in bovine placentomes as a function of gestational age and in retained versus non‐retained placenta

Activity of Placental β‐N‐Acetyl‐Glucosaminidase in Cows with and without Retained Fetal Membranes

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