Activity of insulin growth factors and shrimp neurosecretory organ extracts on a lepidopteran cell line

Hatt, Philippe-Jacques; Liebon, C.; Morinière, Madeleine; Oberlander, Herbert; Porcheron, P.

doi:10.1002/(sici)1520-6327(1997)34:3<313::aid-arch6>3.0.co;2-v

Cited by 25 publications

(11 citation statements)

References 44 publications

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“…The growth-promoting activity of insulin and insulin-like growth factors has been amply documented in both vertebrate cells and organ cultures. In insect cell cultures, exogenous insulin and insulin-like growth factors provoke physiological effects (Seecof and Dewhurst, 1974;Davis and Shearn, 1977;Martin and Shearn, 1980;Mosna, 1981;Wyss, 1982;Cullen and Milner, 1991;Hatt et al, 1994Hatt et al, , 1997 and mammalian insulin is presently a routine additive to Drosophila culture media (Wyss, 1988;Echalier, 1997). Nijhout and Grunert (2002) reported that bombyxin-II stimulates cell division and growth of wing imaginal disks of Precis coenia.…”

Section: Discussionmentioning

confidence: 99%

Effect of bombyxin-II, an insulin-related peptide of insects, on Bombyx mori hemocyte division in single-cell culture

Saito

Iwabuchi

2003

Appl. Entomol. Zool.

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We examined the effect of an insulin-related peptide, bombyxin-II, on the mitotic division of Bombyx mori hemocytes in vitro. Hemocytes isolated from larval hemolymph (day 1 of 4th instar) were cultured singly in MGM-450 medium. Some cultured prohemocytes (11.1%), plasmatocytes (2.6%), granulocytes (8.5%) and spherulocytes (10.8%) underwent mitotic division in the medium. Synthetic bombyxin-II added to the medium together with 10% larval hemolymph notably increased the ratio of dividing granulocytes to 45.5%, while no effect was evident on the other types of hemocytes. Bombyxin-II or hemolymph alone failed to stimulate the division of granulocytes. These results suggest that bombyxin-II acts cooperatively with hemolymph factor(s) to stimulate mitotic division of granulocytes. Bovine pancreas insulin was also active and could substitute for bombyxin.

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Section: Discussionmentioning

confidence: 99%

Effect of bombyxin-II, an insulin-related peptide of insects, on Bombyx mori hemocyte division in single-cell culture

Saito

Iwabuchi

2003

Appl. Entomol. Zool.

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“…Bhakthan & Gilbert 1968, Vanhems et al 1990, Hatt et al 1997, emphasizing some of the pleiotropic functions played by ILPs in animals. More particularly, investigations on the mosquito A. aegypti (Graf et al 1997, Riehle & Brown 1999 revealed ovarian steroidogenesis as a remarkable target of insulin in insects.…”

Section: The Physiological Significance Of the Ovarian Response To Inmentioning

confidence: 99%

Control of ovarian steroidogenesis by insulin-like peptides in the blowfly (Phormia regina)

Manière¹,

Rondot²,

Büllesbach³

et al. 2004

Journal of Endocrinology

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This study investigated the ability of insulin and of insect insulin-like peptides (ILPs) to stimulate ovarian steroidogenesis in the blowfly Phormia regina. Bovine insulin was active on ovaries isolated in vitro, which showed an age-dependent sensitivity; this peptide progressively stimulated steroidogenesis in ovaries isolated from the third day after adult molt, but not in younger ones, and had maximal activity after the fifth day. This stimulatory effect was observed equally from females reared in the presence or in the absence of males, excluding a regulatory effect of mating. The mode of action of insulin in blowflies did not involve cAMP, but triggered a specific and well-conserved transduction cascade. In particular, a peroxovanadium compound, known to activate specifically the insulin receptor in mammals, also stimulated blowfly ovarian steroidogenesis in vitro. Conversely, chemicals known to inhibit the mammalian insulin receptor or downstream elements of its signaling pathway, such as LY294002, an inhibitor of phosphatidylinositol 3-kinase (PI3K), were able to prevent the steroidogenic action of bovine insulin on fly ovaries. Extracts from the median neurosecretory cells (MNCs) of blowfly brains, which are known to contain endogenous ILPs, stimulated ovarian steroidogenesis very efficiently and were also sensitive to inhibition by LY294002. These experiments indicated the involvement of PI3K in the mode of action of MNC extracts and substantiated that their endogenous ILPs are involved in the regulation of ovarian steroidogenesis. This conclusion was corroborated by the effects of synthetic bombyxin II, an ILP originating from silkworm MNCs, which also stimulated steroidogenesis in isolated blowfly ovaries. Altogether, these data suggest that insulinlike neurohormones from MNCs play a crucial role as steroidogenic gonadotropins in female flies.

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“…Our knowledge of the insulin/mTOR pathway in crustaceans and its role in development and growth is fragmentary at best. Insulin-like peptides have been isolated from crustacean tissues (Gallardo et al, 2003;Hatt et al, 1997) and the androgenic gland expresses an insulin-like growth factor that determines male development (Banzai et al, 2011;Manor et al, 2007;Rosen et al, 2010;Sroyraya et al, 2010;. Insulin receptor tyrosine kinases and phosphotyrosyl phosphatases are present in crustacean tissues Chuang and Wang, 1994;Kucharski et al, 1999;Kucharski et al, 2002;Lin et al, 1993).…”

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confidence: 99%

Rheb, an activator of target of rapamycin, in the blackback land crab,Gecarcinus lateralis: cloning and effects of molting and unweighting on expression in skeletal muscle

MacLea

Abuhagr

Pitts

et al. 2012

Journal of Experimental Biology

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SUMMARY Molt-induced claw muscle atrophy in decapod crustaceans facilitates exuviation and is coordinated by ecdysteroid hormones. There is a 4-fold reduction in mass accompanied by remodeling of the contractile apparatus, which is associated with an 11-fold increase in myofibrillar protein synthesis by the end of the premolt period. Loss of a walking limb or claw causes a loss of mass in the associated thoracic musculature; this unweighting atrophy occurs in intermolt and is ecdysteroid independent. Myostatin (Mstn) is a negative regulator of muscle growth in mammals; it suppresses protein synthesis, in part, by inhibiting the insulin/metazoan target of rapamycin (mTOR) signaling pathway. Signaling via mTOR activates translation by phosphorylating ribosomal S6 kinase (s6k) and 4E-binding protein 1. Rheb (Ras homolog enriched in brain), a GTP-binding protein, is a key activator of mTOR and is inhibited by Rheb-GTPase-activating protein (GAP). Akt protein kinase inactivates Rheb-GAP, thus slowing Rheb-GTPase activity and maintaining mTOR in the active state. We hypothesized that the large increase in global protein synthesis in claw muscle was due to regulation of mTOR activity by ecdysteroids, caused either directly or indirectly via Mstn. In the blackback land crab, Gecarcinus lateralis, a Mstn-like gene (Gl-Mstn) is downregulated as much as 17-fold in claw muscle during premolt and upregulated 3-fold in unweighted thoracic muscle during intermolt. Gl-Mstn expression in claw muscle is negatively correlated with hemolymph ecdysteroid level. Full-length cDNAs encoding Rheb orthologs from three crustacean species (G. lateralis, Carcinus maenas and Homarus americanus), as well as partial cDNAs encoding Akt (Gl-Akt), mTOR (Gl-mTOR) and s6k (Gl-s6k) from G. lateralis, were cloned. The effects of molting on insulin/mTOR signaling components were quantified in claw closer, weighted thoracic and unweighted thoracic muscles using quantitative polymerase chain reaction. Gl-Rheb mRNA levels increased 3.4-fold and 3.9-fold during premolt in claw muscles from animals induced to molt by eyestalk ablation (ESA) and multiple leg autotomy (MLA), respectively, and mRNA levels were positively correlated with hemolymph ecdysteroids. There was little or no effect of molting on Gl-Rheb expression in weighted thoracic muscle and no correlation of Gl-Rheb mRNA with ecdysteroid titer. There were significant changes in Gl-Akt, Gl-mTOR and Gl-s6k expression with molt stage. These changes were transient and were not correlated with hemolymph ecdysteroids. The two muscles differed in terms of the relationship between Gl-Rheb and Gl-Mstn expression. In thoracic muscle, Gl-Rheb mRNA was positively correlated with Gl-Mstn mRNA in both ESA and MLA animals. By contrast, Gl-Rheb mRNA in claw muscle was negatively correlated with Gl-Mstn mRNA in ESA animals, and no correlation was observed in MLA animals. Unweighting increased Gl-Rheb expression in thoracic muscle at all molt stages; the greatest difference (2.2-fold) was observed in intermolt animals. There was also a 1.3-fold increase in Gl-s6k mRNA level in unweighted thoracic muscle. These data indicate that the mTOR pathway is upregulated in atrophic muscles. Gl-Rheb, in particular, appears to play a role in the molt-induced increase in protein synthesis in the claw muscle.

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Activity of insulin growth factors and shrimp neurosecretory organ extracts on a lepidopteran cell line

Cited by 25 publications

References 44 publications

Effect of bombyxin-II, an insulin-related peptide of insects, on Bombyx mori hemocyte division in single-cell culture

Effect of bombyxin-II, an insulin-related peptide of insects, on Bombyx mori hemocyte division in single-cell culture

Control of ovarian steroidogenesis by insulin-like peptides in the blowfly (Phormia regina)

Rheb, an activator of target of rapamycin, in the blackback land crab,Gecarcinus lateralis: cloning and effects of molting and unweighting on expression in skeletal muscle

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