Activity of Meropenem with and without Ciprofloxacin and Colistin against
            <i>Pseudomonas aeruginosa</i>
            and
            <i>Acinetobacter baumannii</i>

Pankuch, Glenn A.; Lin, Gengrong; Seifert, Harald; Appelbaum, Peter C.

doi:10.1128/aac.00689-07

Cited by 92 publications

(58 citation statements)

References 25 publications

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“…22,24,25 Colistin activity can be enhanced when combined with antibiotics with different action as carbapenems, rifampicin, ceftazidime. 26,27,28 Present study observd 50% synergism, 30% additive effect, 20% indifference while combining colistin with imipenem. In accordance with the present study Souli et al 29 reported 56.3% synergism and 43.7% indifference.…”

Section: Discussionmentioning

confidence: 99%

In vitro efficacy of synergistic antibiotic combinations in imipenem resistant Pseudomonas aeruginosa strains

Farzana

Shamsuzzaman

2017

Bangladesh J Med Microbiol

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imipenem resistant isolates ranged from > _ 256 mg/L to < _ 8 mg/L for imipenem, > _ 1024 mg/L to < _ 64 mg/L for ceftriaxone, > _ 256 mg/L to < _ 8 mg/L for amikacin, > _ 16 mg/L to < _ 2 mg/L for colistin, > _ 512 mg/L to < _ 16 mg/L for piperacillin/tazobactam. Among antibiotic combinations, piperacillin/tazobactam-amikacin was most effective with 80% synergism next to which was imipenem-amikacin with 60% synergism, then imipenem-colistin with 50% synergism, imipenem-ceftriaxone with 30% synergism. Only one combination (piperacillin/tazobactum -imipenem showed 20% antagonism. All these combinations had considerable proportion of additive effect which is also desirable for these multi drug resistant isolates

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Section: Discussionmentioning

confidence: 99%

In vitro efficacy of synergistic antibiotic combinations in imipenem resistant Pseudomonas aeruginosa strains

Farzana

Shamsuzzaman

2017

Bangladesh J Med Microbiol

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“…Earlier, we reported the prevalence of colistin-resistant isolates (67% of K. pneumoniae) in Tamil Nadu (15), however, to the best of our knowledge, this is the first report on the prevalence of class 1 integrons in colistin-resistant K. pneumoniae in the Southern part of India. The selection of antibiotics colistin-meropenem for the analysis of in vitro combination therapy was based on the phenotypic studies, and chosen antibiotics have differed in mechanism and in the site of action (25,26). Antibiotic combinations of colistin plus rifampin (23), ertapenem plus doripenem (27), tigecycline plus colistin, fosfomycin plus meropenem, and colistin plus carbapenem (imipenem, meropenem) were extensively studied against colistin resistant Klebsiella pneumoniae.…”

Section: Discussionmentioning

confidence: 99%

Colistin-Resistant Klebsiella pneumoniae: Prevalence of Integrons and Synergistic Out Turn for Colistin-Meropenem

Manohar

Shanthini

Pandey

et al. 2018

Arch Clin Infect Dis

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Background: Klebsiella pneumoniae has the potential to disseminate at speed among the hospital environment, hence included as a major nosocomial pathogen causes of severe infections. This work mainly focused on finding out the prevalence of different classed of integrons in colistin-resistant Klebsiellapneumoniae and to analyze the efficacy of colistin-meropenem in combination. Methods: For this cross-sectional study, random non-biased sampling technique was followed and non-repetitive, Kleb-

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“…Killing curves utilizing meropenem, gentamicin, or meropenem plus gentamicin were performed as previously described (8). Briefly, tubes containing cation-supplemented Mueller-Hinton broth with antibiotics at concentrations equal to the MICs of the isolates were seeded with a log-phase bacterial inoculum of 5 ϫ 10 6 CFU/ml (14) and incubated in a shaking water bath at 37°C. Samples (50 l) of this broth culture were removed after 1, 3, 6, 9, 12, and 24 h for enumeration of viable bacteria by serial dilution and plating on antibiotic-free Mueller-Hinton agar plates.…”

mentioning

confidence: 99%

“…The analysis was performed three times for all isolates; the mean values of viable CFU were estimated and plotted on a semilogarithmic graph, and a 24-h time-kill curve was constructed for each isolate. Bactericidal activity (99.9% killing) was defined as a Ն3 log 10 CFU/ml reduction in viable cell counts with respect to the original inoculum (12,14).…”

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confidence: 99%

Characteristics of Meropenem Heteroresistance in Klebsiella pneumoniae Carbapenemase (KPC)-Producing Clinical Isolates of K. pneumoniae

et al. 2010

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Meropenem heteroresistance was investigated in six apparently meropenem-susceptible, Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) clinical isolates, compared with that in carbapenemase-negative, meropenem-susceptible controls. In population analyses, the KPC-KP isolates grew at meropenem concentrations of 64 to 256 g/ml. Heteroresistant colonies had significantly elevated expression of the bla KPC gene compared with the native populations but did not retain heteroresistance when subcultured in drug-free media. Time-kill assays indicated that meropenem alone was not bactericidal against KPC-KP but efficiently killed the control strains.Since the beginning of the last decade, Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) isolates have been increasingly detected in the United States and subsequently in several regions worldwide (3,4,13,17,21). KPC enzymes efficiently hydrolyze all ␤-lactam molecules (1, 22), conferring various levels of resistance to all ␤-lactam compounds, including carbapenems (13). However, KPC-producing K. pneumoniae may appear susceptible to carbapenems, mainly meropenem (2, 13), by reference CLSI agar dilution or broth microdilution methods as well as by automated systems (6,15,17). Characteristically, it has been reported that automated systems may identify as many as 87% of KPC-KP isolates to be susceptible to meropenem (13). The detection of the susceptibility level of KPC-KP isolates to carbapenems has been shown to be difficult due to the phenotypic heterogeneity that they commonly exhibit (3, 10, 13). For instance, in agar diffusion methods such as disk diffusion or Etest, the heterogeneous growth to carbapenems of KPC-KP results in the appearance of scattered colonies within the inhibition zones (9, 13).These issues raise the need for cautious evaluation of susceptibility testing in KPC-KP isolates that are recovered in clinical laboratories. In our clinical laboratories, several KPC-KP isolates that appear susceptible by automated susceptibility assays or reference dilution assays contain heterogeneous subpopulations (D. Sofianou and K. Themeli-Digalaki, personal communications). It has been also shown that among Greek KPC-KP isolates, meropenem tends to exhibit lower MICs than imipenem or ertapenem (17,20). In that respect, the aim of the present study was to characterize the heterogeneous mode of growth of apparently meropenem-susceptible KPC-KP clinical isolates by population analyses and bactericidal assays.Bacterial strains, susceptibility studies, and macrorestriction analysis. Six KPC-2-producing K. pneumoniae clinical isolates from our laboratory collection were randomly selected for the study among those that were meropenem susceptible by agar dilution (MIC Յ 4 g/ml) (5) but exhibited scattered heterogeneous colonies around carbapenem discs. The isolates were recovered from separate patients hospitalized in four hospitals located in different Greek regions. K. pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853, ...

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Activity of Meropenem with and without Ciprofloxacin and Colistin against Pseudomonas aeruginosa and Acinetobacter baumannii

Cited by 92 publications

References 25 publications

In vitro efficacy of synergistic antibiotic combinations in imipenem resistant Pseudomonas aeruginosa strains

In vitro efficacy of synergistic antibiotic combinations in imipenem resistant Pseudomonas aeruginosa strains

Colistin-Resistant Klebsiella pneumoniae: Prevalence of Integrons and Synergistic Out Turn for Colistin-Meropenem

Characteristics of Meropenem Heteroresistance in Klebsiella pneumoniae Carbapenemase (KPC)-Producing Clinical Isolates of K. pneumoniae

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