Activity of Na+/K+-ATPase and the content of phospholipids in the blue mussel Mytilus edulis L. during environmental temperature changes

Немова, Н. Н.; Kaivarainen, Elena I.; Fokina, Natalia

doi:10.1134/s0003683817060114

Cited by 11 publications

(4 citation statements)

References 16 publications

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“…The enhancement of glycerophospholipid metabolism under extreme heat stress in E. malaccana could be important in stabilizing cellular membranes. Glycerophospholipids (mainly phosphatidylethanolamine and phosphatidylcholine) are important components of cell membranes (Nguyen et al, 2020;Overgaard et al, 2008), and have close relations with the permeability of the cell membrane and the activity of ATPases (Nemova et al, 2017). The regulation of glycerophospholipid content under thermal stress may be an adaptive response to warmer temperature (Bolstridge et al, 2010;Hunsberger et al, 2014).…”

Section: Glycerophospholipid Metabolism and Cell Membrane Stabilizationmentioning

confidence: 99%

Temperature adaptations of the thermophilic snail Echinolittorina malaccana: insights from metabolomic analysis

Chen

Wang

Liao

et al. 2021

Journal of Experimental Biology

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The periwinkle snail Echinolittorina malaccana, whose upper lethal temperature is near 55°C, is one of the most heat-tolerant eukaryotes known. We conducted a multi-level investigation, including cardiac physiology, enzyme activity, and targeted and untargeted metabolomic analysis, that elucidated a spectrum of adaptations to extreme heat. All systems examined showed heat intensity-dependent responses. Under moderate heat stress (from 37 to 45°C) the snail depressed cardiac activity and entered a state of metabolic depression. The global metabolomic analyses and enzymatic analysis showed that the depressed metabolic state under moderate heat stress entailed production of metabolites characteristic of oxygen-independent pathways of ATP generation (lactate and succinate), which suggests that anaerobic metabolism was the main energy supply pathway under heat stress (from 37 to 52°C). The metabolomic analyses also revealed alterations in glycerophospholipid metabolism under extreme heat stress of 52°C, which likely reflected adaptive changes to maintain membrane structure. Small molecular mass organic osmolytes (glycine betaine, choline, and carnitine) showed complex changes in concentration that were consistent with a role of these protein-stabilizing solutes in protection of the proteome under heat stress. This thermophilic species thus can deploy a wide array of adaptive strategies to acclimatize to extremely high temperatures.

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Section: Glycerophospholipid Metabolism and Cell Membrane Stabilizationmentioning

confidence: 99%

Temperature adaptations of the thermophilic snail Echinolittorina malaccana: insights from metabolomic analysis

Chen

Wang

Liao

et al. 2021

Journal of Experimental Biology

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“…Some literature has noted that the Na + /K + -ATPase gene in the snail might play a critical role in response to low-temperature treatment [23]. The activity of the Na + /K + -ATPase gene in the gills and hepatopancreas of Mytilus edulis has a good correlation with phospholipid content [41]. In this research, a Na + /K + -ATPase gene was observed with downregulation in Guangdong snails.…”

Section: Candidate Cold-resistance Genes Related To Membranementioning

confidence: 48%

A Comparative Transcriptomics Approach to Analyzing the Differences in Cold Resistance in Pomacea canaliculata between Guangdong and Hunan

Liu

Sun

Wang

et al. 2020

Journal of Immunology Research

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Pomacea canaliculata, known as an invasive freshwater snail, is also called a golden apple snail; its survival and expansion are greatly affected by temperature. In this study, high-throughput sequencing (RNA-seq) was used to perform comparative transcriptome analysis on the muscular tissue (G_M) of snails in Guangdong and Hunan. Differential gene screening was performed with FDR <0.05 and |log2FoldChange| >1 as the threshold, and a total of 1,368 differential genes were obtained (671 genes showed upregulation in snails from Guangdong, and 697 genes displayed upregulation in snails from Hunan). Fifteen genes were identified as candidate genes for the cold hardiness of Pomacea canaliculata. Among them, three genes were involved in energy metabolism (glycogen synthase, 1; DGK, 1; G6PD, 1); seven genes were involved in homeostasis regulation (HSP70, 2; BIP, 1; GPX, 1; GSTO 1, G6PD, 1; caspase-9, 1); two genes were involved in amino acid metabolism (glutamine synthetase, 1; PDK, 1); and four genes were involved in membrane metabolism (inositol-3-phosphate synthase, 1; Na+/K+-ATPase, 1; calcium-binding protein, 2). This study presents the molecular mechanisms for the cold hardiness of Pomacea canaliculata, which could provide a scientific basis for the forecast and prevention of harm from Pomacea canaliculata.

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“…Previous study exhibited that phospholipids can affect the growth and survival of larvae fish, accompanied by changes of Na + /K + ‐ATPase activity (Chen et al, ). Related research also indicated that phospholipid content in gills of Eriocheir sinensis plays an important role in regulating the activity of Na + /K + ‐ATPase (Nemova, Kaivarainen, & Fokina, ). The present study revealed that Na + /K + ‐ATPase activity of juvenile shrimps significantly increased by dietary PC.…”

Section: Discussionmentioning

confidence: 99%

Effect of the dietary phosphatidylcholine at different growth stages of Pacific white shrimps,Litopenaeus vannamei

et al. 2019

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This study investigated the effects of dietary phosphatidylcholine (PC) at different growth stages of Litopenaeus vannamei (L. vannamei) with an initial weight of 0.70 ± 0.00 g (juvenile) and 4.25 ± 0.00 g (subadult) for 8 weeks. Six isonitrogenous and isoenergetic diets containing PC (0, 1.7, 5.1, 6.8, 10.2 and 13.6 g/kg) were confected using formulated with redfish meal, dehulled soybean meal and peanut meal as protein source, fish oil and corn oil as fat source. The juveniles were reared in 0.3 m3 aquarium at the density of 40 in triplicates, as well as the subadults in 0.5 m3 at the density of 40 in triplicates. Results showed PC level had significant effects on weight gain rate (WGR), specific growth rate, feed conversion ratio in juvenile and subadult shrimps and hepatosomatic index in juvenile shrimps (p < 0.05). No significant differences were found in moisture, whole body protein and crude ash (p > 0.05). Crude fat of juveniles had no significant change (p > 0.05), but subadults increased significantly (p < 0.05). Both juveniles and subadults showed that triglyceride and low‐density lipoprotein presented a downward trend with PC elevating, but high‐density lipoprotein increased gradually. Cholesterol in juveniles presented upward, but downward in subadults (p < 0.05). Dietary PC also significantly reduced the aspartate transaminase in juveniles and alanine transaminase in subadults (p < 0.05). PC supplementation significantly improved the glutathione S‐transferase activity and reduced the content of malondialdehyde in the hepatopancreas (p < 0.05). PC supplementation significantly improved gill filament sodium‐potassium adenosine triphosphatase enzyme of juvenile shrimps (p < 0.05), but subadult shrimps were not influenced (p > 0.05). PC supplementation significantly elevated the content of hepatopancreas eicosapentaenoic acid, highly unsaturated fatty acid (HUFA) and n‐3HUFA (p < 0.05), but the content of linoleic acid significantly reduced (p < 0.05). The broken‐line and quadratic regression model based on WGR showed the optimum dietary PC requirement was 10.3 g/kg and 6.5 g/kg for juveniles and subadults, respectively.

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Activity of Na+/K+-ATPase and the content of phospholipids in the blue mussel Mytilus edulis L. during environmental temperature changes

Cited by 11 publications

References 16 publications

Temperature adaptations of the thermophilic snail Echinolittorina malaccana: insights from metabolomic analysis

Temperature adaptations of the thermophilic snail Echinolittorina malaccana: insights from metabolomic analysis

A Comparative Transcriptomics Approach to Analyzing the Differences in Cold Resistance in Pomacea canaliculata between Guangdong and Hunan

Effect of the dietary phosphatidylcholine at different growth stages of Pacific white shrimps,Litopenaeus vannamei

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