Activity of NAD-kinase of nymph Triatoma infestants upon treatment with DDT and other compounds

Ilevicky, Jovita; Dinamarca, Maria Luisa; Agosín, Manuel R.

doi:10.1016/0010-406x(64)90110-0

Cited by 20 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since then much evidence has been gathered to support this hypothesis (Ortega, 1962;Hart and Pouts, 1965;Morello, 1964). Agosin et al (1965) have found that protein biosynthesis is stimulated by DDT, but not by DDE in nymphal Triatoma infestans, which appeared to be consistent with the observations of Ilevicky et al (1964) that DDT stimulated NADkinase activity in T. infestans. Our results indicated an increased synthesis of the demethylase system in the chick as well.…”

Section: Discussionmentioning

confidence: 61%

Chick microsomal oxidases. Isolation, properties, and stimulation by embryonic exposure to 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane

Abou‐Donia¹,

Menzel²

1968

Biochemistry

View full text Add to dashboard Cite

The optimum conditions for the activity of the chick liver microsomal oxidative , -dimethylaniline demethylase have been established. Reduced nicotinamide-adenine dinucleotide phosphate was required and was partially replaced by reduced nicotinamide-adenine dinucleotide. The enzyme was competitively inhibited by /3-diethylaminoethyl diphenylpropylacetate. Michaelis constants in the absence and in the presence of /3-diethylaminoethyl diphenylpropylacetate were determined for , -dimethylaniline and , -dimethylaniline A-oxide. The data presented in this work demonstrate that the rate of , -dimethylaniline A-oxide demethylation by chick liver microsomal fraction is sufficiently high for the A-oxide to be considered as an intermediate in the oxidative demethylation of , -dimethylaniline. Ferrous ions activated the microsomal demethylase when incubated with nicotinamide-adenine dinucleotide phosphate and gave a greater oxidative reaction in the absence of nicotinumerous studies have been conducted on the oxidative demethylation of A-methyl compounds. In the presence of NADPH and oxygen the microsomal fraction catalyzed the oxidation of some of the A-

show abstract

Section: Discussionmentioning

confidence: 61%

Chick microsomal oxidases. Isolation, properties, and stimulation by embryonic exposure to 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane

Abou‐Donia¹,

Menzel²

1968

Biochemistry

View full text Add to dashboard Cite

show abstract

“…To briefly review the doses we and others have found to be required to show induction, we begin with the work of Agosin and his colleagues (Ilevicky et al, 1964), who were the first to study this problem in insects. These workers used DDT at 300 µg/bug to demonstrate the induction of NAD-kinase in T. infestans.…”

Section: Discussionmentioning

confidence: 99%

“…They found that DDT increased the level of NADP, which is an important cofactor in microsomal oxidation. Later, they showed that the increased level of NADP following DDT treatment resulted from increased activity of NAD-kinase (Ilevicky et al, 1964). These workers suggested that, since DDT was metabolized in some species by the microsomal oxidases (Agosin et al, 1961), its induction of this system might be related to DDT resistance.…”

mentioning

confidence: 99%

Induction of detoxifying enzymes in insects

Terriere¹,

Yu²

1974

J. Agric. Food Chem.

View full text Add to dashboard Cite

“…There are several metabolic pathways for D D T in insects (Perry, 19641, one of which involves microsomal NAD-kinase. The activity of this enzyme in Triatoma infestans increases within a few hours of treatment with DDT, and Ilivicky, Dinamarca & Agosin (1964) showed that this is probably due to the production of additional enzyme, and not to activation. D D T stimulates protein synthesis (Agosin, Aravena & Neghme, 1965), and this is at least part of the explanation for NAD-kinase induction, although there could theoretically also be decreased degradation (Agosin, Ilivicky & Litvak, 1967).…”

Section: (4) Enzyme Inductionmentioning

confidence: 97%

The Sublethal Effects of Synthetic Insecticides on Insects

Moriarty

1969

Biological Reviews

100

View full text Add to dashboard Cite

Summary The synthetic insecticides have been used in steadily increasing quantities since the Second World War and are non‐specific poisons, and so pose the possibility of sublethal effects on wild populations of animals. The organochlorine insecticides in general, and some of the cyclodienes in particular, are usually much more persistent than the organophosphorus and carbamate insecticides, both in the environment and within individual insects. Analyses of samples suggest that traces of the organochlorines are widely dispersed over the entire globe. Although the evidence is incomplete, all of these synthetic insecticides appear to exert their primary toxic lesion by interference with the conduction of impulses in the nervous system. The complete pattern of events that ends in death includes the release of secondary toxins into the blood. These are probably physiologically active compounds, normally present, but liberated in excessive amounts by excessive nervous stimulation. A condition similar to the late, paralytic, stage of insecticidal poisoning can also be produced, in Periplaneta americana, by excessive physical stimulation and there are similarities in the physiological changes caused by both types of stimuli. This suggests that a generalized stress syndrome develops after excessive nervous stimulation. However, this similarity has so far only been demonstrated for this one insect species. Sublethal effects have so far only been looked for cursorily. Interpretation of sublethal effects is sometimes difficult; apparent differences between dosed and control individuals may sometimes be caused by selective mortality of the dosed individuals. Four main types of effect have been found. Latent toxicity, which is evident as a reduction of longevity or of death at particular developmental stages, has been found only with organochlorines, appears to be linked with fat metabolism, and shows clearly that sublethal effects may be produced a long time after acquiring a dose of a persistent insecticide. All types of synthetic insecticide can affect reproductive potential, and may increase or decrease the number of eggs produced. Egg fertility or development of offspring may also be affected. Reductions are sometimes caused directly by inhibition or distortion of ovary development, sometimes indirectly by reduced feeding. The few studies on behaviour have shown that activity may increase, feeding decrease, and the acceptance threshold of sucrose be lowered. DDT induces the formation of more detoxifying microsomal enzyme in Triatoma infestans. I suggest that latent toxicity and some behavioural changes are probably caused by direct effects on the nervous system, but that some of the other results may be secondary effects associated with the stress syndrome. There is also the possibility of direct insecticidal action on other systems. It is a pleasure to thank our librarian, Mrs Jean King, for her unfailing help in tracing references.

show abstract

Activity of NAD-kinase of nymph Triatoma infestants upon treatment with DDT and other compounds

Cited by 20 publications

References 15 publications

Chick microsomal oxidases. Isolation, properties, and stimulation by embryonic exposure to 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane

Chick microsomal oxidases. Isolation, properties, and stimulation by embryonic exposure to 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane

Induction of detoxifying enzymes in insects

The Sublethal Effects of Synthetic Insecticides on Insects

Contact Info

Product

Resources

About