Activity of photosynthetic Reaction Centers coated with polydopamine

Milano, Francesco; Lopresti, Marco; Vona, Danilo; Buscemi, Gabriella; Cantore, Mariangela; Farinola, Gianluca M.; Trotta, Massimo

doi:10.1557/adv.2020.275

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…Dopamine monomers have been recently utilized to modify electrode materials, such as carbon nanotubes providing adhesive properties for the deposition of thylakoids composites for biophotocurrent generation, and silk fibroin electrodes in microbial fuel cells . The entrapment of isolated photosynthetic reaction centers from purple bacteria in PDA films or nanoaggregates for biophotoanode preparation has also been reported, , where diffusible exogenous redox mediators were required to obtain PDA–enzyme biohybrid systems for energy purposes. PDA has also been utilized to modify various electrode surfaces for application in microbial electrochemical systems, enhancing surface area and bacterial cell loading, decreasing startup times, and accelerating interfacial electron transfer .…”

Section: Introductionmentioning

confidence: 99%

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Buscemi

Vona

Stufano

et al. 2022

ACS Appl. Mater. Interfaces

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Interfacing intact and metabolically active photosynthetic bacteria with abiotic electrodes requires both establishing extracellular electron transfer and immobilizing the biocatalyst on electrode surfaces. Artificial approaches for photoinduced electron harvesting through redox polymers reported in literature require the separate synthesis of artificial polymeric matrices and their subsequent combination with bacterial cells, making the development of biophotoanodes complex and less sustainable. Herein, we report a one-pot biocompatible and sustainable approach, inspired by the byssus of mussels, that provides bacterial cells adhesion on multiple surfaces under wet conditions to obtain biohybrid photoanodes with facilitated photoinduced electron harvesting. Purple bacteria were utilized as a model organism, as they are of great interest for the development of photobioelectrochemical systems for H 2 and NH 3 synthesis, biosensing, and bioremediation purposes. The polydopamine matrix preparation strategy allowed the entrapment of active purple bacteria cells by initial oxygenic polymerization followed by electrochemical polymerization. Our results unveil that the deposition of bacterial cells with simultaneous polymerization of polydopamine on the electrode surface enables a 5-fold enhancement in extracellular electron transfer at the biotic/abiotic interface while maintaining the viability of the cells. The presented approach paves the way for a more sustainable development of biohybrid photoelectrodes.

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Section: Introductionmentioning

confidence: 99%

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Buscemi

Vona

Stufano

et al. 2022

ACS Appl. Mater. Interfaces

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“…Interestingly, PDA not only forms surface adhered films, but also solution suspended nanoparticles (NPs) whose diameter can be controlled tuning DA polymerization conditions such as pH, temperature and monomer concentration. [18] Like in films, also PDA NPs surface can be further modified via metal coordination, electrostatic interactions, covalent or hydrogen bonding, π-π stacking, [19] for a variety of applications ranging from biomedicine to sensing. [20] However, PDA NPs are subjected to sedimentation and show optical opalescence and a dark color due to strong light absorption.…”

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confidence: 99%

Polydopamine/Ethylenediamine Nanoparticles Embedding a Photosynthetic Bacterial Reaction Center for Efficient Photocurrent Generation

Buscemi

Vona

Ragni

et al. 2021

Advanced Sustainable Systems

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Biohybrid nanostructures based on photoenzymes from photosynthetic organisms, which are able to efficiently convert solar light into charge separated states, open up new concepts in the design of sustainable systems for solar energy conversion. [1] In particular, bacterial reaction centers (RC), such as the RC obtained from the purple photosynthetic bacterium Rhodobacter (R.) sphaeroides, have raised interest for these applications since they are easy to isolate and purify and they can be handled outside their native environment without loss of functionality. [2] More in detail, the bacterial RC as the one used in this study [3] is a membrane spanning protein composed of three subunits non-covalently bound to a series of cofactors arranged in two symmetrical branches A and B (Figure 1).Upon photoexcitation, a dimer (D) of bacteriochlorophylls absorbing at 865 nm transfers, with remarkable unitary quantum yield, an electron along the A branch to a bacteriochlorophyll (absorbing at 800 nm), then to a bacteriopheophytin (absorbing at 760 nm) and finally to the so-called quinone acceptor complex composed of two ubiquinone-10 (UQ 10 ) molecules located in the Q A and Q B pockets, respectively. The ratio of the band absorbance at 760, 800 and 865 nm is 1:2:1 in the intact protein. The final charge separated state (eitheris blocked or empty) has deep absorption minimum at 865 nm in the light-dark difference spectrum suitable to study the protein photoactivity. In the presence of the physiological electron donor cytochrome c 2 , or artificial donors such as ferrocene and derivatives, RC photoactivation leads to a fully reduced and fully protonated UQ 10 H 2 molecule at the Q B site, that leaves the pocket and can be replaced by another UQ 10 or analogous ubiquinone molecules present in solution. Such photocycle is often exploited in photoelectrochemical cells, [1b,5] capable to convert sunlight into electrical energy, recently reaching a record of 1.3 mA cm −2 in hybrid systems involving transition metal-semiconductor Schottky junctions. [6] The photocycle can be reproduced using isolated RC suspended in direct, [2a,7] or inverse micellar systems, [8] but it can Photoactive biohybrid soft nanoparticles are obtained by embedding the Rhodobacter sphaeroides Reaction Center (RC) in polydopamine (PDA) suspended aggregates and treating them with ethylenediamine (EDA). Such PDA:EDA@ RC nanoparticles are investigated for photocurrent generation in photoelectrochemical cells, which are able to convert sunlight into electrical energy. The photosynthetic protein retains its structural and functional integrity in the nanostructures and the PDA:EDA@RC nanoparticles exhibit better water dispersity, improved light collection ability, and higher photocurrent generation compared to the PDA@RC precursors, where the reaction center is embedded in pure PDA. The hybrid soft nanoparticles incorporating the RC bacterial photoenzyme show charge separated state generation comparable to that of the pristine enzyme in solution, overcoming t...

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Halotolerance of Rhodobacter sphaeroides for saline and hypersaline wastewater bioremediation

Labarile

Farinola

Varsalona

et al. 2021

2021 International Workshop on Metrology for the Sea; Learning to Measure Sea Health Parameters (MetroSea)

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Activity of photosynthetic Reaction Centers coated with polydopamine

Cited by 4 publications

References 35 publications

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Bio-Inspired Redox-Adhesive Polydopamine Matrix for Intact Bacteria Biohybrid Photoanodes

Polydopamine/Ethylenediamine Nanoparticles Embedding a Photosynthetic Bacterial Reaction Center for Efficient Photocurrent Generation

Halotolerance of Rhodobacter sphaeroides for saline and hypersaline wastewater bioremediation

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