Activity of serum cytidine deaminase during pregnancy

Jones, D. D.; Bahijri, Suhad; Roberts, Elton L.; Williams, Geoffrey F.

doi:10.1111/j.1471-0528.1982.tb04703.x

Cited by 21 publications

(15 citation statements)

References 4 publications

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“…3.5.4.12) and had demonstrated elevated dCMP deaminase activity in patients with pre-eclampsia and other causes of high-risk pregnancies Jones, 1975,1982). CD is more stable than deoxycytidylate deaminase and Jones et al (1982) claimed that the discrimination between normal and high-risk pregnancies was as good when CD was measured as when dCMP deaminase was used. Baines and Clark (1985) also investigated the use of serum CD as a marker for pre-eclampsia using a similar method but with the ammonia determined after incubation of serum with substrate for only 2 h at 37 "C, using the glutamate dehydrogenase end-point method (1975).…”

Section: Cytidine Deaminasementioning

confidence: 98%

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The measurement of nucleoside deaminases by high performance liquid chromatography and their use in clinical chemistry

Sherwood¹

1991

Biomedical Chromatography

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The measurement of the nucleoside deaminases--cytidine deaminase, guanosine deaminase and adenosine deaminase--by reversed phase high performance liquid chromatography is reviewed. The clinical value of assaying the enzyme activity is discussed for each of these enzymes. Both cytidine deaminase and adenosine deaminase measurements have proven clinical value, although the use of the assay of cytidine deaminase in the diagnosis of pre-eclampsia is probably not helpful.

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Section: Cytidine Deaminasementioning

confidence: 98%

“…The early work on CD was carried out using the method first described by Jones et al (1982). This involved incubation of serum (100 pL) with deoxycytidine in carbonate/bicarbonate buffer (pH 9.2) for 18 h at 22 "C. The ammonia produced was estimated using the Berthelot reaction.…”

Section: Cytidine Deaminasementioning

confidence: 99%

The measurement of nucleoside deaminases by high performance liquid chromatography and their use in clinical chemistry

Sherwood¹

1991

Biomedical Chromatography

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“…It is therefore for this reason that a single serum was added to the Standards. A similar technique has been described by Jones et al (4).…”

Section: Influence Of Serum On the Berthelot Reactionmentioning

confidence: 99%

“…SF4-006FG5) was used for estimatifig the cat alytic activity concentration of serum lkaline phosphatase (37 °C). Cytidine deaminase (22 0 C) was assayed aecording to Jones et al (4).…”

Section: Other Enzyme Assaysmentioning

confidence: 99%

The Estimation of Serum Guanosine Deaminase Activity in Liver Disease

Jones¹,

Roberts²,

Davies³

1983

CCLM

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Summary: An assay for the estimation of guanosine deaminase is described. The method employs guanosine äs Substrate and after incubation of serum and Substrate at 22 °C for 18 h the ammonia Hberated is estimated using the Berthelot reaction. Absorbance is measured at 625 nm and the catalytic activity read from a Standard curve obtained using ammonia Standards. The method provides reproducible measurement of serum guanosine deaminase. The results obtained using 'normal' sera have been used to calculate the 'normal ränge' for the enzyme in serum. Preliminary results suggest that guanosine deaminase is increased in hepatitis and in patients with liver metastases but normal in all other liver diseases including cirrhosis and obstructive jaundice. Bestimmung von Guanosindesaminase im Serum bei LebererkrankungenZusammenfassung: Eine Methode zur Bestimmung von Guanosindesaminase wird beschrieben. Serum wird mit Guanosin als Substrat inkubiert und nach 18 h bei 22 °C wird das freigesetzte Ammoniak mit der /terfAefof-Reaktion bestimmt. Die Absorption wird bei 625 nm gemessen und die katalytische Aktivität aus einer mit Ammoniak-Standards erstellten Standardkurve ermittelt. Die mit "NormaP'-Seren erhaltenen Ergebnisse wurden zur Berechnung des "Normale-Bereichs für das Enzym im Serum verwendet. Vorläufige Ergebnisse weisen daraufhin, daß Guanosindesaminase bei Hepatitis und bei Patienten mit Lebermetastasen erhöhtj bei allen anderen Erkrankungen der Leber, eingeschlossen Cirrhose und Verschlußikterus, jedoch normal ist.

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“…Measurement of the activity of CD in serum was proposed as a new method for the early detection of pre-eclamptic toxaemia, cholestasis of pregnancy and intra-uterine death (Jones et al, 1982). However, other investigators have reported a poor correlation of elevated CD activity with pre-eclampsia (Baines and Clark, 1985;Sherwood et a l , 1987).…”

Section: Introductionmentioning

confidence: 97%

Determination of plasma cytidine deaminase activity by HPLC

Richards

Sherwood

Ndebele

et al. 1987

Biomedical Chromatography

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Cytidine deaminase is an enzyme of nucleic acid metabolism, the measurement of which has been proposed as a useful test for the early detection of pre-eclamptic toxaemia in pregnancy. The enzyme converts the nucleoside cytidine to uridine, with the release of ammonia, and it is the measurement of this latter compound that forms the basis of the conventional methods for the assay of cytidine deaminase. The low activity of the enzyme requires long incubation times, which in turn increase the possibility of contamination by exogenous ammonia. We have developed a new method for determining cytidine deaminase activity, utilising high performance liquid chromatography to measure the production of uridine. This method uses much shorter incubation times and is unaffected by ammonia contamination. This paper describes the development of the method and its comparison with the established assay. The relative merits of each are discussed. Finally, the adaptation of incubation and chromatographic conditions, in order to measure other enzymes of nucleic acid metabolism which are of clinical interest, is briefly mentioned.

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Activity of serum cytidine deaminase during pregnancy

Cited by 21 publications

References 4 publications

The measurement of nucleoside deaminases by high performance liquid chromatography and their use in clinical chemistry

The measurement of nucleoside deaminases by high performance liquid chromatography and their use in clinical chemistry

The Estimation of Serum Guanosine Deaminase Activity in Liver Disease

Determination of plasma cytidine deaminase activity by HPLC

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