Activity of sitafloxacin against extracellular and intracellular Staphylococcus aureus in vitro and in vivo: comparison with levofloxacin and moxifloxacin

Shi, Gongming; Chen, Xiangdong; Wang, Hui; Wang, Siwen; Guo, Xin; Zhang, Xulei

doi:10.1038/ja.2012.7

Cited by 11 publications

(7 citation statements)

References 24 publications

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“…In this study, the MIC and MBC values of Catechin and Catechin-Cu nanoparticles were evaluated by broth microdilution method 20 21 . Table 1 shows the MIC and MBC of Catechin and Catechin-Cu nanoparticles against various microorganisms.…”

Section: Resultsmentioning

confidence: 99%

Enhancing the antimicrobial activity of natural extraction using the synthetic ultrasmall metal nanoparticles

Chen

Zhao

et al. 2015

Sci Rep

167

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The use of Catechin as an antibacterial agent is becoming ever-more common, whereas unstable and easy oxidation, have limited its application. A simple and low-energy-consuming approach to synthesize highly stable and dispersive Catechin-Cu nanoparticles(NPs) has been developed, in which the stability and dispersivity of the NPs are varied greatly with the pH value and temperature of the reaction. The results demonstrate that the optimal reaction conditions are pH 11 at room temperature. As-synthesized NPs display excellent antimicrobial activity, the survival rates of bacterial cells exposed to the NPs were evaluated using live/dead Bacterial Viability Kit. The results showed that NPs at the concentration of 10 ppm and 20 ppm provided rapid and effective killing of up to 90% and 85% of S. aureus and E. coli within 3 h, respectively. After treatment with 20 ppm and 40 ppm NPs, the bacteria are killed completely. Furthermore, on the basis of assessing the antibacterial effects by SEM, TEM, and AFM, it was found the cell membrane damage of the bacteria caused by direct contact of the bacteria with the NPs was the effective mechanism in the bacterial inactivation.

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Section: Resultsmentioning

confidence: 99%

Enhancing the antimicrobial activity of natural extraction using the synthetic ultrasmall metal nanoparticles

Chen

Zhao

et al. 2015

Sci Rep

167

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“…To confirm the antibacterial effect in mice, an MRSA infected peritonitis model was used and performed with minor modification as described previously. 18,19 Mice were infected with 5×10 7 CFU of MRSA WHO-2 by intraperitoneal injection. After infection for 2 hours, antibiotic treatments were administered subcutaneously.…”

Section: Assessment Of Exolzd Activity In Vivomentioning

confidence: 99%

Exosome-encapsulated antibiotic against intracellular infections of methicillin-resistant <em>Staphylococcus aureus</em>

Yang¹,

Shi²,

Guo³

et al. 2018

IJN

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BackgroundStaphylococcus aureus survival inside phagocytes is considered to provide a reservoir of bacteria that are relatively protected from antibiotics, thus enabling long-term colonization of the host and explaining clinical failures and relapses after antibiotic therapy.PurposeThe objective of this study was to develop a nanovesicle using exosomes loaded with linezolid to overcome intracellular infections by pathogenic bacteria.MethodsExosomes were collected from the culture supernatants of RAW 264.7 cells. Their size distribution and zeta potential were characterized by dynamic light scattering, their morphology was characterized by transmission electron microscopy, and their protein content (CD63 and Flotillin 1) was assessed by Western blotting. Linezolid was incorporated into exosomes by co-incubation at 37°C and it’s accumulation in RAW264.7 cells and release in vitro were determined by high performance liquid chromatography. The intracellular bactericidal effect was evaluated in methicillin-resistant S. aureus (MRSA)-infected macrophages in vitro and MRSA peritonitis model in vivo.ResultsWe prepared a nanoformulation of the antibiotic linezolid using exosomes harvested from mouse RAW264.7 macrophages. The exosomal formulation of linezolid was more effective against intracellular MRSA infections in vitro and in vivo than the free linezolid. Our data also showed no signs of cytotoxicity in macrophages.ConclusionExosomes provide an effective alternative for intracellular antibiotic delivery of antibiotic that is efficacious, cost-effective, and safe. This regimen can be viewed as a potential antimicrobial agent for use against intracellular infections.

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“…Moxifloxacin, used as monotherapy, has been beneficial in secondary severe intra-abdominal sepsis with high clinical and bacteriological efficacies in hospitalized patients; even with polyorganic insufficiency 7 .This fourth generation fluor quinolone promotes control of sepsis and septic-shock and has a good penetration from the blood into the peritoneal cavity, aiming to control severe peritonitis [21][22][23][24][25][26] . However, its use inside the peritoneal cavity has not yet been tested.…”

Section: Discussionmentioning

confidence: 99%

Severe autogenously fecal peritonitis in Wistar rats with permanent bilateral carotid occlusion. Response to intra peritoneal moxifloxacin combined with dexamethasone

Melo

Gadelha²,

Oliveira

et al. 2014

Acta Cir. Bras.

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PURPOSE:To evaluate the treatment outcome of severe peritonitis in rats submitted to permanent bilateral carotid occlusion (PBCO). METHODS:Sixteen Wistar rats (mean age of 8.5 months) with PBCO underwent autogenously fecal peritonitis, and were treated with moxifloxacin combined with dexamethasone, and followed-up for 45 days. Ten rats (mean age five months) without PBCO were used as a control group. The variables were expressed by their mean and standard error of the mean (SEM). p<0.05 was used for rejecting the null hypothesis. The study was approved by the Ethics Committee. RESULTS:There was a significant increase (p=0.0002) in the mortality and morbidity in older rats that underwent PBCO (study group). However, even among the survival rats presenting with severe residual abscesses both in the abdomen and thorax cavities, they present an almost normal life. CONCLUSIONS:The treatment of severe autogenously fecal peritonitis with intraperitoneal moxifloxacin combined with dexamethasone was very effective in young rats without permanent bilateral carotid occlusion. The treatment reached reasonable results in older rats with PBCO, even considering residual abscesses on abdomen and thorax. Older age was the greater risk factor for the outcome of the treatment of severe peritonitis. Sepsis remains a challenging situation, especially in elderly.Key words: Peritonitis. Quinolones. Dexamethasone. Therapy. Rats. Severe autogenously fecal peritonitis in Wistar rats with permanent bilateral carotid occlusion.Response to intra peritoneal moxifloxacin combined with dexamethasone

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Activity of sitafloxacin against extracellular and intracellular Staphylococcus aureus in vitro and in vivo: comparison with levofloxacin and moxifloxacin

Cited by 11 publications

References 24 publications

Enhancing the antimicrobial activity of natural extraction using the synthetic ultrasmall metal nanoparticles

Enhancing the antimicrobial activity of natural extraction using the synthetic ultrasmall metal nanoparticles

Exosome-encapsulated antibiotic against intracellular infections of methicillin-resistant <em>Staphylococcus aureus</em>

Severe autogenously fecal peritonitis in Wistar rats with permanent bilateral carotid occlusion. Response to intra peritoneal moxifloxacin combined with dexamethasone

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