Activity of sodium trimetaphosphate, associated or not with fluoride, on dual-species biofilms

Cavazana, Thamires Priscila; Hosida, Thayse Yumi; Pessan, Juliano Pelim; Sampaio, Caio; Monteiro, Douglas Roberto; Delbem, Alberto Carlos Botazzo

doi:10.1080/08927014.2019.1653455

Cited by 18 publications

(26 citation statements)

References 41 publications

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“…Yassin and co-workers (2016) used this to develop fluoride releasing dental polymer that was able to inhibit the growth of C. albicans-Lactobacillus casei-S. mutans PMBs. Sodium trimetaphosphate is a supplement to fluoride in oral health and has beneficial effects on tooth enamel, but its effect on oral biofilms are not well studied (Cavazana et al, 2019), although it could reduce the metabolic activity and production of EXM by C. albicans-S. mutans biofilms. When combined with fluoride, it was also able to reduce biofilm biomass significantly.…”

Section: In Search Of Novel Therapeutic Solutions To Polymicrobial Bi...mentioning

confidence: 99%

Recent Advances and Opportunities in the Study of Candida albicans Polymicrobial Biofilms

Pohl

2022

Front. Cell. Infect. Microbiol.

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It is well known that the opportunistic pathogenic yeast, Candida albicans, can form polymicrobial biofilms with a variety of bacteria, both in vitro and in vivo, and that these polymicrobial biofilms can impact the course and management of disease. Although specific interactions are often described as either synergistic or antagonistic, this may be an oversimplification. Polymicrobial biofilms are complex two-way interacting communities, regulated by inter-domain (inter-kingdom) signaling and various molecular mechanisms. This review article will highlight advances over the last six years (2016-2021) regarding the unique biology of polymicrobial biofilms formed by C. albicans and bacteria, including regulation of their formation. In addition, some of the consequences of these interactions, such as the influence of co-existence on antimicrobial susceptibility and virulence, will be discussed. Since the aim of this knowledge is to inform possible alternative treatment options, recent studies on the discovery of novel anti-biofilm compounds will also be included. Throughout, an attempt will be made to identify ongoing challenges in this area.

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Section: In Search Of Novel Therapeutic Solutions To Polymicrobial Bi...mentioning

confidence: 99%

Recent Advances and Opportunities in the Study of Candida albicans Polymicrobial Biofilms

Pohl

2022

Front. Cell. Infect. Microbiol.

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“…The actions of TMP and F in the anti-caries process have already been well established [ 10 , 33 , 34 , 35 ]. Studies have shown that TMP can be adsorbed on the enamel surface, thereby reducing demineralization because this process can hinder acid diffusion and alter the affinity between enamel and salivary proteins [ 36 , 37 , 38 ]. TMP adsorbs into the enamel, forming a reticular layer that covers the enamel surface and decreases Ca phosphate apatite precipitation within the enamel pores.…”

Section: Discussionmentioning

confidence: 99%

“…The virulence factors of this microorganism are acidogenic (mainly production of lactic acid) and aciduric properties. In addition, S. mutans uses sucrose from the diet to synthesize large amounts of extracellular polysaccharides, mostly glucans, synthesized by glucosyltransferases [ 38 , 51 , 52 ]. C. albicans , in contrast, is an opportunistic pathogenic fungus [ 51 , 53 ], and its virulence is related to the transition from its yeast form to hyphae [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…The association of TMP and F with the extract may have contributed to its antimicrobial action. Cavazzana et al showed that TMP and F reduced the number of viable S. mutans cells, and TMP (0.25% without F) decreased the total biomass and extracellular matrix components of C. albicans and S. mutans biofilms [ 38 ]. Another interesting finding in our study is that the biofilm pH after treatment for 1 min and 10 min was acidic, mainly for biofilms treated with formulations ETF2 and TF2, and, even with these values, we observed reductions between 1 and 2 logs for the C. albicans and S. mutans biofilms when treated with the developed formulations.…”

Section: Discussionmentioning

confidence: 99%

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Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate

et al. 2022

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This study investigated the anti-caries and anti-inflammatory effects of mouthwash formulations containing Punica granatum (pomegranate) peel extract (PPE), sodium-trimetaphosphate, and low concentrations of fluoride. PPE was characterized using high-performance liquid chromatography (ellagic acid and punicalagin). Total phenolics were quantified among formulations, and their stability was analyzed for 28 days. The formulation effects were evaluated as follows: (1) inorganic component concentration and reduced demineralization on bovine enamel blocks subjected to pH cycling; (2) anti-biofilm effect on dual-biofilms of Streptococcus mutans ATCC 25175 and Candida albicans ATCC 10231 treated for 1 and 10 min, respectively; and (3) cytotoxicity and production of inflammatory mediators (interleukin-6 and tumor necrosis factor-alpha). The formulation containing 3% PPE, 0.3% sodium-trimetaphosphate, and 225 ppm of fluoride resulted in a 34.5% surface hardness loss; a 13% (treated for 1 min) and 36% (treated for 10 min) biofilm reduction in S. mutans; a 26% (1 min) and 36% (10 min) biofilm reduction in C. albicans; absence of cytotoxicity; and anti-inflammatory activity confirmed by decreased interleukin-6 production in mouse macrophages. Thus, our results provide a promising prospect for the development of an alcohol-free commercial dental product with the health benefits of P. granatum that have been recognized for a millennium.

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“…Furthermore, studies evaluating the effects of TMPm, with or without F, on dual-species S. mutans and C. albicans biofilms have demonstrated that this association maintains the biofilm pH close to neutral even under highly cariogenic conditions, in addition to increasing F and P concentrations in the biofilm fluid [ 16 ]. It is noteworthy that although TMPm and F do not affect the microbial viability of such biofilms [ 17 ], TMP and F combination substantially reduces the expression of the ECM components, the biofilm’s metabolism and inorganic components of these biofilms, which play crucial roles in dental caries dynamics [ 17 ]. The effects of TMPm are further enhanced after administering this phosphate as nanoparticles (TMPn), in which TMPn combined with F increases the protective effect of fluoride vehicles against dental de-mineralization, in addition to improving their re-mineralization patterns compared to fluoride vehicles containing TMPm [ 14 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Activity of Sodium Trimetaphosphate Nanoparticles on Cariogenic-Related Biofilms In Vitro

et al. 2022

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In light of the promising effect of sodium trimetaphosphate nanoparticles (TMPn) on dental enamel, in addition to the scarce evidence of the effects of these nanoparticles on biofilms, this study evaluated the activity of TMPn with/without fluoride (F) on the pH, inorganic composition and extracellular matrix (ECM) components of dual-species biofilms of Streptococcus mutans and Candida albicans. The biofilms were cultivated in artificial saliva in microtiter plates and treated with solutions containing 1% or 3% conventional/microparticulate TMP (TMPm) or TMPn, with or without F. After the last treatment, the protein and carbohydrate content of the ECM was analyzed, and the pH and F, calcium (Ca), phosphorus (P), and TMP concentrations of the biofilms were determined. In another set of experiments, after the last treatment, the biofilms were exposed to a 20% sucrose solution, and their matrix composition, pH, and inorganic component contents were evaluated. 3% TMPn/F significantly reduced ECM carbohydrate and increased biofilm pH (after sucrose exposure) than other treatments. Also, it significantly increased P and F levels before sucrose exposure in comparison to 3% TMPm/F. In conclusion, 3% TMPn/F affected the biofilm ECM and pH, besides influencing inorganic biofilm composition by increasing P and F levels in the biofilm fluid.

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Activity of sodium trimetaphosphate, associated or not with fluoride, on dual-species biofilms

Cited by 18 publications

References 41 publications

Recent Advances and Opportunities in the Study of Candida albicans Polymicrobial Biofilms

Recent Advances and Opportunities in the Study of Candida albicans Polymicrobial Biofilms

Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate

Activity of Sodium Trimetaphosphate Nanoparticles on Cariogenic-Related Biofilms In Vitro

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