Activity of soluble factors produced by Epstein-Barr virus-transformed human B lymphocytes on different cell lines

Abstract

Self-stimulatory growth factors, produced by a human Epstein-Barr virus (EBV)-positive lymphoblastoid B cell line, named BA-D10-4, have been tested for the capacity to induce DNA synthesis in various human and animal cell lines, including lymphoid, either EBV-positive or EBV-negative, and non-lymphoid cell lines. It has been found that BA-D10-4 cells produce growth factors which seem to be essential for their sustained proliferation in vitro, and which increase DNA synthesis in different primate lymphoid cells, independently of the presence of the EBV genome and of the lymphocyte lineage.

Epstein-Barr virus-transformed B lymphocytes produce low molecular mass molecules with autocrine growth factor and competence factor activity

“…BA-D10-4 is a cloned line producing human IgM monoclonal antibody to mycobacterial antigen which was established in our laboratory from the peripheral blood cells of a tuberculin skin test-positive healthy donor, according to a method described in detail elsewhere [12]. The activity of soluble factors produced by BA-D10-4 cells on different human and animal cells lines has recently been reported [13].…”