Activity of ß-1,3-glucanase and ß-1,4-glucanase in two potato cultivars following challenge by the fungal pathogen Alternaria solani

Shahbazi, Hadis; Aminian, Heshmatolah; Sahebani, Navazollah; Halterman, Dennis

doi:10.1007/s12600-011-0184-2

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…Pathogenesis-related (PR) proteins are important in plant defense against pathogens, some PR proteins have been identified as chitinases and β-1,3-glucanases [21] . The high activities of these two proteins was potentially related to resistance to Alternaria solani in potato [22] . The synthesis of PR proteins is regulated at the transcriptional level and it has often been suggested that the collection of PR proteins may be effective in inhibiting pathogen growth and inducing systemic acquired resistance [23,24] .…”

Section: Introductionmentioning

confidence: 99%

Fusaricidins in Paenibacillus polymyxa A21 and their antagonistic activity against Botrytis cinerea on tomato

Liu¹,

Wu²,

X³

et al. 2018

Front. Agr. Sci. Eng.

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Four kinds of antifungal compounds from an extract of Paenibacillus polymyxa A21 with molecular masses of 883.56, 897.59, 947.55, and 961.58 Da were characterized as the members of fusaricidin-type of antibiotics according to LC-MS analysis. Fusaricidins isolated from culture filtrate displayed high antagonistic activity against several plant fungal pathogens, especially Botrytis cinerea, the causal agent of gray mold. The fusaricidins biosynthetic gene cluster (BGC) from A21 was cloned by PCR and comparative cluster analysis revealed that gene fusTE, the 3′ boundary of the fusaricidin BGC in strain PKB1, was not present in fusaricidin BGC of A21, indicating that fusTE is not necessary for fusaricidin synthesis. Fusaricidin extract from A21 significantly reduced gray mold disease incidence and severity on tomato. The mRNA levels for three pathogenesis-related proteins (PRs) revealed that treatment of tomato leaves with fusaricidin extract induced the expression of PR genes to different levels, suggesting that one reason for the reduction of gray mold infection by fusaricidin is induction of PR proteins, which lead to increased resistance to pathogens. This is the first report of the application of fusaricidins to control tomato gray mold and the comparative cluster analysis provides important molecular basis for research on fusaricidin biosynthesis.

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Section: Introductionmentioning

confidence: 99%

Fusaricidins in Paenibacillus polymyxa A21 and their antagonistic activity against Botrytis cinerea on tomato

Liu¹,

Wu²,

X³

et al. 2018

Front. Agr. Sci. Eng.

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Efficient transformation and expression of the glucanase gene fromBacillus megateriumin the biocontrol strainStreptomyces lydicusA02

Dong

et al. 2013

Biocontrol Science and Technology

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Genome sequence of the potato pathogenic fungus Alternaria solani HWC-168 reveals clues for its conidiation and virulence

Zhang

Haddadi

et al. 2018

BMC Microbiol

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BackgroundAlternaria solani is a known air-born deuteromycete fungus with a polycyclic life cycle and is the causal agent of early blight that causes significant yield losses of potato worldwide. However, the molecular mechanisms underlying the conidiation and pathogenicity remain largely unknown.ResultsWe produced a high-quality genome assembly of A. solani HWC-168 that was isolated from a major potato-producing region of Northern China, which facilitated a comprehensive gene annotation, the accurate prediction of genes encoding secreted proteins and identification of conidiation-related genes. The assembled genome of A. solani HWC-168 has a genome size 32.8 Mb and encodes 10,358 predicted genes that are highly similar with related Alternaria species including Alternaria arborescens and Alternaria brassicicola. We identified conidiation-related genes in the genome of A. solani HWC-168 by searching for sporulation-related homologues identified from Aspergillus nidulans. A total of 975 secreted protein-encoding genes, which might act as virulence factors, were identified in the genome of A. solani HWC-168. The predicted secretome of A. solani HWC-168 possesses 261 carbohydrate-active enzymes (CAZy), 119 proteins containing RxLx[EDQ] motif and 27 secreted proteins unique to A. solani.ConclusionsOur findings will facilitate the identification of conidiation- and virulence-related genes in the genome of A. solani. This will permit new insights into understanding the molecular mechanisms underlying the A. solani-potato pathosystem and will add value to the global fungal genome database.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1324-3) contains supplementary material, which is available to authorized users.

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Activity of ß-1,3-glucanase and ß-1,4-glucanase in two potato cultivars following challenge by the fungal pathogen Alternaria solani

Cited by 3 publications

References 23 publications

Fusaricidins in Paenibacillus polymyxa A21 and their antagonistic activity against Botrytis cinerea on tomato

Fusaricidins in Paenibacillus polymyxa A21 and their antagonistic activity against Botrytis cinerea on tomato

Efficient transformation and expression of the glucanase gene fromBacillus megateriumin the biocontrol strainStreptomyces lydicusA02

Genome sequence of the potato pathogenic fungus Alternaria solani HWC-168 reveals clues for its conidiation and virulence

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