Activity of the endophytic fungi Phlebia sp. and Paecilomyces formosus in decolourisation and the reduction of reactive dyes’ cytotoxicity in fish erythrocytes

Abstract

The current study investigates the potential for discolouration and degradation of Reactive Blue 19 and Reactive Black 5 textile dyes by endophytic fungi Phlebia sp. and Paecilomyces formosus as well as the potential cytotoxicity of products or by-products generated by the treatments in fish erythrocytes. It was observed at 30 days that both endophytes showed biodegradation activity with 0.1 g mL of dyes. P. formosus showed highest extracellular and intracellular protein content levels after the 15th day, and Phlebia sp. stands out for production of extracellular laccase, indicating that this enzyme may be associated with the decolouration capacity. The dyes showed toxic effects in fishes at 0.01 g mL concentration, resulting in the appearance of micronuclei in erythrocyte cells. When degraded dyes treated by endophytes were tested, the frequency of micronuclei reduced approximately 20%, indicating the effectiveness of these endophytic in the treatment of textile dyes with less environmental impact, thus indicating a potential for application of these fungi in bioremediation process.

Endangerment of Ostrya rehderiana Chun and its relationship with rhizosphere soil microflora

“…For plant root growth, an arbuscular mycorrhizal fungus Gigaspora was found to be beneficial (Vohník, Fendrych, Albrechtová, & Vosátka, 2007). Other beneficial aspects are Meyerozyma inhibits anthracnose (Chen et al., 2016) and Phlebia degrades toxic substances (Bulla et al., 2017; Kamei, Sonoki, Haraguchi, & Kondo, 2006). These genera are not found in the rhizosphere soil of wild and planted Or.…”

Bacterias y hongos endófitos de la familia Cactaceae y sus aplicaciones

“…Estas características de importancia ecológica han enfocado los estudios de estas especies en su conservación, principalmente proponiendo distintas formas de propagación y cultivo in vitro. En los últimos diez años, se ha incrementado el estudio sobre la actividad biológica de algunos de los metabolitos secundarios producidos por estas plantas, así como la identificación y actividad de su microbioma (Bezerra et al, 2013;Chávez-Ambriz, Hernández-Morales, Cabrera-Luna, Luna-Martínez & Pacheco-Aguilar, 2016;Bulla et al, 2017;Eke et al, 2019;Camarena-Pozos, Flores-Núñez, López & Partida-Martínez, 2021).…”

“…Por otro lado, existen hongos que degradan colorantes de aguas residuales a través de exoenzimas o por bioabsorción (Cardona, Osorio & Quintero, 2009). Algunas de esas enzimas son lacasas provenientes de hongos endófitos y no endófitos, demostrándose su potencial para degradar tintes de origen industrial (Bulla et al, 2017;Bettin et al, 2019 textiles utilizados, más que las otras cepas analizadas. Este género de hongo endófito ya ha sido identificado en algunas cactáceas, como se mencionó en párrafos anteriores (Bezerra et al, 2013;Ratnweera et al, 2015;Bertetti et al, 2017); por lo tanto, se sugiere que sería posible aislar una enzima o compuesto similar a la lacasa en esas u otras especies de cactus.…”

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“…Endophytes have great importance in the production of compounds with a unique structure, which may result from several biological interactions [47], however, the challenge for drug discovery is how to access this chemical potential. Most of the studies on endophytic biodiversity published in Brazil (2012-2017) present some bioprospecting studies, such as evaluation of antibiotic, antioxidant, antiparasitic, or cytotoxicity activities, or the use of endophytes to promote plant growth or reactive dye discoloration [40,[48][49][50][51][52][53][54] (▶ Table 3). As a result of the success obtained in terms of discovering active metabolite-producing endophytes, a large number of compounds [2,[55][56][57] or known compounds with unreported biological properties [33,36,[58][59][60] have been reported.…”