Activity of the Glutathione Antioxidant System and NADPH-Generating Enzymes in Blood Serum of Rats with Type 2 Diabetes Mellitus after Administration of Melatonin-Correcting Drugs

Agarkov, A. A.; Попова, Т. Н.; Веревкин, А.Н.; Matasova, L. V.

doi:10.1007/s10517-014-2524-y

Cited by 10 publications

(10 citation statements)

References 6 publications

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“…It was then incubated and monitored for 10 min at 37°C and the absorbance was read at 340 nm. GSSG-R activity is expressed in U/min/mL of plasma [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

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Infusion ofHibiscus sabdariffa L.Modulates Oxidative Stress in Patients with Marfan Syndrome

Soto

Zúñiga-Muñoz

Guarner-Lans

et al. 2016

Mediators of Inflammation

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Marfan syndrome (MFS) is associated with progressive aortic dilatation, endothelial dysfunction, and oxidative stress that contribute to the early acute dissection of the vessel and can end up in rupture of the aorta and sudden death. Many studies have described that the organic acids from Hibiscus sabdariffa Linne (HSL) calyces increase cellular antioxidant capacity and decrease oxidative stress. Here we evaluate if the antioxidant properties of HSL infusion improve oxidative stress in MFS patients. Activities of extra cellular super oxide dismutase (ECSOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione reductase (GSSG-R), glutathione (GSH), lipid peroxidation (LPO) index, total antioxidant capacity (TAC), and ascorbic acid were determined in plasma from MFS patients. Values before and after 3 months of the treatment with 2% HSL infusion were compared in control and MFS subjects. After treatment, there was a significant decrease in ECSOD (p = 0.03), EGPx (p = 0.04), GST (p = 0.03), GSH (p = 0.01), and TAC and ascorbic acid (p = 0.02) but GSSG-R activity (p = 0.04) and LPO (p = 0.02) were increased in MFS patients in comparison to patients receiving the HSL treatment and C subjects. Therefore, the infusion of HSL calyces has antioxidant properties that allow an increase in antioxidant capacity of both the enzymatic and nonenzymatic systems, in the plasma of the MSF patients.

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“…It was then incubated and monitored for 10 min at 37°C and the absorbance was read at 340 nm. GSSG-R activity is expressed in U/min/mL of plasma [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

“…Then the reduction of GSSG to GSH is catalyzed by glutathione reductase (GSSG-R) [ 11 ]. This GSH system protects from overproduction of ROS under pathological conditions [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Infusion ofHibiscus sabdariffa L.Modulates Oxidative Stress in Patients with Marfan Syndrome

Soto

Zúñiga-Muñoz

Guarner-Lans

et al. 2016

Mediators of Inflammation

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“…Due to altered levels of SCFAs, Type 2 diabetes confers a threefold increased risk for the development of TB. Individuals with Type 2 diabetes mellitus and HIV infection also have compromised levels of glutathione (GSH) [ 61 , 71 , 72 ]. In the case of HIV infected individuals, this leads to an inappropriate immune response by promoting a CD4 + T-helper 2 (T H 2) directed response, which results in cytokines that ineffectively defend against intracellular pathogens such as M. tb .…”

Section: Hiv Infection and Type 2 Diabetes Association With Neutromentioning

confidence: 99%

Effector Mechanisms of Neutrophils within the Innate Immune System in Response to Mycobacterium tuberculosis Infection

Warren

Teskey

Venketaraman

2017

JCM

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Neutrophils have a significant yet controversial role in the innate immune response to Mycobacterium tuberculosis (M. tb) infection, which is not yet fully understood. In addition to neutrophils’ well-known effector mechanisms, they may also help control infection of M. tb through the formation of neutrophil extracellular traps (NETs), which are thought to further promote the killing of M. tb by resident alveolar macrophages. Cytokines such as IFN-γ have now been shown to serve an immunomodulatory role in neutrophil functioning in conjunction to its pro-inflammatory function. Additionally, the unique transcriptional changes of neutrophils may be used to differentiate between infection with M. tb and other bacterial and chronic rheumatological diseases such as Systemic Lupus Erythematosus. Adversely, during the innate immune response to M. tb, inappropriate phagocytosis of spent neutrophils can result in nonspecific damage to host cells due to necrotic lysis. Furthermore, some individuals have been shown to be more genetically susceptible to tuberculosis (TB) due to a “Trojan Horse” phenomenon whereby neutrophils block the ability of resident macrophages to kill M. tb. Despite these aforementioned negative consequences, through the scope of this review we will provide evidence to support the idea that neutrophils, while sometimes damaging, can also be an important component in warding off M. tb infection. This is exemplified in immunocompromised individuals, such as those with human immunodeficiency virus (HIV) infection or Type 2 diabetes mellitus. These individuals are at an increased risk of developing tuberculosis (TB) due to a diminished innate immune response associated with decreased levels of glutathione. Consequently, there has been a worldwide effort to limit and contain M. tb infection through the use of antibiotics and vaccinations. However, due to several significant limitations, the current bacille Calmette-Guerin vaccine (BCG, vaccine against TB) does not meet the criteria for universal utilization for all ages and populations across the globe. New research involving neutrophils has yielded a new vaccine called M. smegmatis-Ag85C-MPT51-HspX (mc2-CMX) that has been shown to elicit a humoral and cellular response against M. tb in mice that is superior to the BCG vaccine.

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“…At present, reduced GSH has been widely applied in the drug treatment and adjuvant therapy of a variety of multi-system, multi-organ diseases such as those associated with liver and kidney damage, eye diseases, and cardiovascular diseases 9) . As an antioxidant, reduced GSH lowers the activity of redox-sensitive NF-kB, inhibits inflammatory cytokines (e.g., tumor necrosis factor-, interleukin-6, interleukin-8), and attenuates oxidative stress injury 10) . In this study, after diabetic rabbits were given intravenous injections of reduced GSH for 4 weeks, there was no significant difference between groups according to the results of general observation or micro-CT scanning.…”

Section: Experimental Dm Is Produced By Various Methods Includingmentioning

confidence: 99%

Reduced Glutathione Promotes Implants Osseointegration in Rabbits with Diabetes Mellitus: An Experimental Study

Zhang

2015

J. Hard Tissue Biology.

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To observe the effects of reduced glutathione on promoting osseointegration of titanium implants in New Zealand rabbits with type I diabetes mellitus and to provide novel evidence for improving implants osseointegration. Diabetes mellitus models were established by injecting alloxan (80 mg/kg) into New Zealand rabbits. Success was verified by the blood glucose level. Titanium implants were then placed in the rabbits' femoral epiphysis. Intravenous glutathione (GSH, 30 mg/kg) or saline, respectively, was started. The rabbits were divided into four groups: GSH-treated diabetes mellitus (GSH+DM), diabetes mellitus (DM), GSH-treated normal rabbits (GSH), normal control rabbits (NC). Four weeks after surgery, the rabbits were evaluated via general observation, micro-computed tomography (micro-CT), H&E staining and bone morphogenetic protein 2 (BMP-2) immumohistochemical staining. In results, general observation and micro-CT showed that wounds had healed, no implants had loosened, and there was good synostosis in all groups. Micro-CT also revealed that peri-implant threaded bone density was higher than that in peri-implant cancellous bone and was similar to that in femoral cortical bone. The implants tightly fused with surrounding bone tissue. HE staining revealed that large numbers of bone trabeculaes, osteoblasts, and chondrocytes in the GSH+DM group and granulation tissue, mononuclear macrophages, and multinucleated giant cells in the DM group. New bone trabecular formation was rare. There was greater expression of BMP-2 in the GSH+DM group than in the GM group. The results suggest that GSH promotes new peri-implant bone formation and osseointegration in diabetic rabbits.

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Activity of the Glutathione Antioxidant System and NADPH-Generating Enzymes in Blood Serum of Rats with Type 2 Diabetes Mellitus after Administration of Melatonin-Correcting Drugs

Cited by 10 publications

References 6 publications

Infusion ofHibiscus sabdariffa L.Modulates Oxidative Stress in Patients with Marfan Syndrome

Infusion ofHibiscus sabdariffa L.Modulates Oxidative Stress in Patients with Marfan Syndrome

Effector Mechanisms of Neutrophils within the Innate Immune System in Response to Mycobacterium tuberculosis Infection

Reduced Glutathione Promotes Implants Osseointegration in Rabbits with Diabetes Mellitus: An Experimental Study

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