Activity of upper electron-excited states in bioluminescence of coelenterates

Belogurova, Nadezhda V.; Alieva, R.R.; Kudryasheva, Nadezhda S.

doi:10.1016/j.molstruc.2008.11.014

Cited by 9 publications

(4 citation statements)

References 27 publications

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“…Energy migration involving upper electron-excited states in biological processes is a question of special interest. In our previous studies, we have discussed and experimentally proved involvement of upper electron-excited states of fluorophores to bioluminescent reactions of coelenterates [21] and bacteria [22,47,48]. Current study connects fluorescent activity of upper electron-excited states with structural peculiarities of low-weight biological molecules, free and protein-bound.…”

Section: Discussionmentioning

confidence: 97%

“…Genetically modified forms of obelin with different bioluminescence color have been developed [20]. Activity of upper electron-excited states of the enzyme-bound CLM in the obelin bioluminescence reaction has been demonstrated experimentally [21]; similarity to the excitation processes of other luminous organisms (bacteria and fireflies) has been discussed [21,22].…”

Section: Introductionmentioning

confidence: 99%

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Ultraviolet fluorescence of coelenteramide and coelenteramide-containing fluorescent proteins. Experimental and theoretical study

Alieva

Tomilin

Кузубов

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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Coelenteramide-containing fluorescent proteins are products of bioluminescent reactions of marine coelenterates. They are called 'discharged photoproteins'. Their light-induced fluorescence spectra are variable, depending considerably on external conditions. Current work studies a dependence of light-induced fluorescence spectra of discharged photoproteins obelin, aequorin, and clytin on excitation energy. It was demonstrated that photoexcitation to the upper electron-excited states (260-300nm) of the discharged photoproteins initiates a fluorescence peak in the near UV region, in addition to the blue-green emission. To characterize the UV fluorescence, the light-induced fluorescence spectra of coelenteramide (CLM), fluorophore of the discharged photoproteins, were studied in methanol solution. Similar to photoproteins, the CLM spectra depended on photoexcitation energy; the additional peak (330nm) in the near UV region was observed in CLM fluorescence at higher excitation energy (260-300nm). Quantum chemical calculations by time depending method with B3LYP/cc-pVDZ showed that the conjugated pyrazine-phenolic fragment and benzene moiety of CLM molecule are responsible for the additional UV fluorescence peak. Quantum yields of CLM fluorescence in methanol were 0.028±0.005 at 270-340nm photoexcitation. A conclusion was made that the UV emission of CLM might contribute to the UV fluorescence of the discharged photoproteins. The study develops knowledge on internal energy transfer in biological structures - complexes of proteins with low-weight aromatic molecules.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Ultraviolet fluorescence of coelenteramide and coelenteramide-containing fluorescent proteins. Experimental and theoretical study

Alieva

Tomilin

Кузубов

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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“…These reactions are Ca 2+ dependent, and this is a basis for their biomedical application [42,43]. The biochemical and photophysical mechanisms of the bioluminescence reactions [44,45,46,47,48,49] and spectral characteristics of their products, CLM-containing proteins [50,51,52,53,54,55,56,57], are now under intensive investigation.…”

Section: Coelenteramide-containing Fluorescent Protein As the Simpmentioning

confidence: 99%

Monitoring of Low-Intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions, and Fluorescent Proteins

Kudryasheva

Kovel

2019

IJMS

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The current paper reviews the applications of luminescence bioassays for monitoring the results of low-intensity exposures which produce a stimulative effect. The impacts of radioactivity of different types (alpha, beta, and gamma) and bioactive compounds (humic substances and fullerenols) are under consideration. Bioassays based on luminous marine bacteria, their enzymes, and fluorescent coelenteramide-containing proteins were used to compare the results of the low-intensity exposures at the cellular, biochemical, and physicochemical levels, respectively. High rates of luminescence response can provide (1) a proper number of experimental results under comparable conditions and, therefore, proper statistical processing, with this being highly important for “noisy” low-intensity exposures; and (2) non-genetic, i.e., biochemical and physicochemical mechanisms of cellular response for short-term exposures. The results of cellular exposures were discussed in terms of the hormesis concept, which implies low-dose stimulation and high-dose inhibition of physiological functions. Dependencies of the luminescence response on the exposure time or intensity (radionuclide concentration/gamma radiation dose rate, concentration of the bioactive compounds) were analyzed and compared for bioassays of different organization levels.

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“…The reactions are Ca-dependent, and this is a basis for their biomedical application [34][35]. Biochemical and photophysical mechanisms of the bioluminescence reactions [36][37][38][39][40][41] and spectral characteristics of their products -coelenteramide-containing proteins [42][43][44][45][46][47][48][49] are under intensive investigations now.…”

Section: Introductionmentioning

confidence: 99%

Low-intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions and Fluorescent Proteins

Kudryasheva¹,

Kovel²

2019

Preprint

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Current paper reviews applications of luminescence bioassays for monitoring low-intensity factors, namely, radioactivity of different types (alpha, beta and gamma), and bioactive compounds (humic substances and fullerenols). Luminescence intensity is taken as a physiological parameter of luminous organisms. High rates of luminescence response can provide (1) a proper number of experiments under comparable conditions and, therefore, proper statistical processing, with this being highly important for ‘noisy’ low-dose exposures; (2) non-genetic, i.e. biochemical and physicochemical mechanisms of cellular response, in accordance to “exposome” concept. Bioassays based on luminous marine bacteria, their enzymes, and fluorescence coelenteramide-containing proteins were used to compare results of low-intensity exposures at cellular, biochemical and physicochemical levels, respectively. Results of the cellular exposures were discussed in terms of hormesis concept. Bioluminescence time dependence under low-dose radiation exposures corresponded to hormesis or threshold models; no bioluminescence monotonic dependency on intensity of exposure (dose rate, radioactivity, concentration) was found. Bioluminescence activation and absence of its dependency on intensity of exposure can be accepted as features of cellular adaptive response. Changes of biological luminescence were analyzed and discussed for bioassays of lower organization level – enzymes and florescent protein.

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Activity of upper electron-excited states in bioluminescence of coelenterates

Cited by 9 publications

References 27 publications

Ultraviolet fluorescence of coelenteramide and coelenteramide-containing fluorescent proteins. Experimental and theoretical study

Ultraviolet fluorescence of coelenteramide and coelenteramide-containing fluorescent proteins. Experimental and theoretical study

Monitoring of Low-Intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions, and Fluorescent Proteins

Low-intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions and Fluorescent Proteins

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