Activity patterns of cardiomyocytes in electrospun scaffolds of poly (ε-caprolactone), collagen, and epicatechin

Miranda-Buendia, E.; González-Gómez, G.H.; Falcón, M.A; Durán-Pastén, María Luisa; Jiménez‐Martínez, Cristian; Vera‐Graziano, Ricardo; Ospina-Orejarena, A.; Rivera-Torres, F.; Prado-Villegas, G.; Maciel-Cerda, A.

doi:10.1016/j.mtcomm.2022.103405

Cited by 4 publications

(1 citation statement)

References 45 publications

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“…The pure PVA and hybrid scaffolds were cut into 0.25 cm 2 pieces and sterilized under UV-C light for 25 min in 7.5 cm diameter Petri dishes into which dissociated cardiomyocytes were seeded. Primary cell culture was performed according to previously established procedures [ 34 , 35 , 36 , 37 , 38 ]. Ventricular cells collected from embryonic chick hearts in ovo at 7–8 days were enzymatically dissociated to obtain a density of 4 × 10 4 cells/mL.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Modulation of Spontaneous Activity in Embryonic Cardiomyocytes Cultured on Poly(vinyl alcohol)/Bioglass Type 58S Electrospun Scaffolds

Rivera-Torres,

Maciel-Cerda,

González-Gómez

et al. 2024

Nanomaterials

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Because of the physiological and cardiac changes associated with cardiovascular disease, tissue engineering can potentially restore the biological functions of cardiac tissue through the fabrication of scaffolds. In the present study, hybrid nanofiber scaffolds of poly (vinyl alcohol) (PVA) and bioglass type 58S (58SiO2-33CaO-9P2O5, Bg) were fabricated, and their effect on the spontaneous activity of chick embryonic cardiomyocytes in vitro was determined. PVA/Bg nanofibers were produced by electrospinning and stabilized by chemical crosslinking with glutaraldehyde. The electrospun scaffolds were analyzed to determine their chemical structure, morphology, and thermal transitions. The crosslinked scaffolds were more stable to degradation in water. A Bg concentration of 25% in the hybrid scaffolds improved thermal stability and decreased degradation in water after PVA crosslinking. Cardiomyocytes showed increased adhesion and contractility in cells seeded on hybrid scaffolds with higher Bg concentrations. In addition, the effect of Ca2+ ions released from the bioglass on the contraction patterns of cultured cardiomyocytes was investigated. The results suggest that the scaffolds with 25% Bg led to a uniform beating frequency that resulted in synchronous contraction patterns.

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Section: Methodsmentioning

confidence: 99%

In Vitro Modulation of Spontaneous Activity in Embryonic Cardiomyocytes Cultured on Poly(vinyl alcohol)/Bioglass Type 58S Electrospun Scaffolds

Rivera-Torres,

Maciel-Cerda,

González-Gómez

et al. 2024

Nanomaterials

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Electroactive Nanomaterials for the Prevention and Treatment of Heart Failure: From Materials and Mechanisms to Applications

Kong,

Guo,

Teng

et al. 2024

Small

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Heart failure (HF) represents a cardiovascular disease that significantly threatens global well‐being and quality of life. Electroactive nanomaterials, characterized by their distinctive physical and chemical properties, emerge as promising candidates for HF prevention and management. This review comprehensively examines electroactive nanomaterials and their applications in HF intervention. It presents the definition, classification, and intrinsic characteristics of conductive, piezoelectric, and triboelectric nanomaterials, emphasizing their mechanical robustness, electrical conductivity, and piezoelectric coefficients. The review elucidates their applications and mechanisms: 1) early detection and diagnosis, employing nanomaterial‐based sensors for real‐time cardiac health monitoring; 2) cardiac tissue repair and regeneration, providing mechanical, chemical, and electrical stimuli for tissue restoration; 3) localized administration of bioactive biomolecules, genes, or pharmacotherapeutic agents, using nanomaterials as advanced drug delivery systems; and 4) electrical stimulation therapies, leveraging their properties for innovative pacemaker and neurostimulation technologies. Challenges in clinical translation, such as biocompatibility, stability, and scalability, are discussed, along with future prospects and potential innovations, including multifunctional and stimuli‐responsive nanomaterials for precise HF therapies. This review encapsulates current research and future directions concerning the use of electroactive nanomaterials in HF prevention and management, highlighting their potential to innovating in cardiovascular medicine.

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Epicatechin Derivatives in Tissue Engineering: Antioxidant, Anti-Inflammatory, Regenerative Use

Miranda Buendia,

González-Gómez,

Maciel-Cerda

et al. 2024

Tissue Engineering Part B: Reviews

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Activity patterns of cardiomyocytes in electrospun scaffolds of poly (ε-caprolactone), collagen, and epicatechin

Cited by 4 publications

References 45 publications

In Vitro Modulation of Spontaneous Activity in Embryonic Cardiomyocytes Cultured on Poly(vinyl alcohol)/Bioglass Type 58S Electrospun Scaffolds

In Vitro Modulation of Spontaneous Activity in Embryonic Cardiomyocytes Cultured on Poly(vinyl alcohol)/Bioglass Type 58S Electrospun Scaffolds

Electroactive Nanomaterials for the Prevention and Treatment of Heart Failure: From Materials and Mechanisms to Applications

Epicatechin Derivatives in Tissue Engineering: Antioxidant, Anti-Inflammatory, Regenerative Use

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