Actomyosin contractility spatiotemporally regulates actin network dynamics in migrating cells

Okeyo, Kennedy Omondi; Adachi, Taiji; Sunaga, Junko; Hojo, Masaki

doi:10.1016/j.jbiomech.2009.07.002

Cited by 45 publications

(49 citation statements)

References 40 publications

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“…8c) phase. Although such values are in agreement with the ones experimentally found Okeyo et al 2009;Wilson et al 2010), some remarks may be done. First, a smaller velocity during the protrusion phase is due to the fact that the extension mainly occurs locally at the pseudopod domain Ω ppod,i (p), according to the chosen mode of deformation (Eq.…”

Section: Temporal Sensing Modelsupporting

confidence: 87%

Cell Migration with Multiple Pseudopodia: Temporal and Spatial Sensing Models

Allena

2013

Bull Math Biol

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is an open access repository that collects the work of Arts et Métiers ParisTech researchers and makes it freely available over the web where possible. Abstract Cell migration triggered by pseudopodia (or "false feet") is the most used method of locomotion. A 3D finite element model of a cell migrating over a 2D substrate is proposed, with a particular focus on the mechanical aspects of the biological phenomenon. The decomposition of the deformation gradient is used to reproduce the cyclic phases of protrusion and contraction of the cell, which are tightly synchronized with the adhesion forces at the back and at the front of the cell, respectively. First, a steady active deformation is considered to show the ability of the cell to simultaneously initiate multiple pseudopodia. Here, randomness is considered as a key aspect, which controls both the direction and the amplitude of the false feet. Second, the migration process is described through two different strategies: the temporal and the spatial sensing models. In the temporal model, the cell "sniffs" the surroundings by extending several pseudopodia and only the one that receives a positive input will become the new leading edge, while the others retract. In the spatial model instead, the cell senses the external sources at different spots of the membrane and only protrudes one pseudopod in the direction of the most attractive one.

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Section: Temporal Sensing Modelsupporting

confidence: 87%

Cell Migration with Multiple Pseudopodia: Temporal and Spatial Sensing Models

Allena

2013

Bull Math Biol

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“…Similar to the force produced by smooth muscle contraction, the force necessary for cell body contractility during migration is developed by the cross-bridge movement of actomyosin, which is initiated by RLC phosphorylation (57,58). As an essential kinase for RLC phosphorylation, MLCK is thought to accelerate cell migration by regulating multiple processes, including retraction (59), actin retrograde flow (32), and membrane ruffling (16).…”

Section: Discussionmentioning

confidence: 99%

Myosin Light Chain Kinase (MLCK) Regulates Cell Migration in a Myosin Regulatory Light Chain Phosphorylation-independent Mechanism

Chen

Tao

Wen

et al. 2014

Journal of Biological Chemistry

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Background: MLCK in cell migration remains controversial. Results: MLCK deletion causes enhanced cell protrusion along with a reduction of membrane tension and is rescued by kinase-dead MLCK or five-DFRXXL motif. Conclusion: MLCK regulates cell migration not by myosin regulatory light chain phosphorylation but possibly through a membrane tension-based mechanism. Significance: Our results shed light on a novel regulatory mechanism of protrusion during cell migration.

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“…the lamellipodium (growth zone), and myosin-dependent contraction and actin depolymerization at the rear of the lamellipodium (the contractile zone) [4,12]. These processes are characterized by rates of growth at the front of the cell (g f ) or contraction at the back of the cell (g b ), respectively, and produce force between different parts of the cell.…”

Section: Introductionmentioning

confidence: 99%

Damped and persistent oscillations in a simple model of cell crawling

Bayly

Taber

Carlsson

2011

J. R. Soc. Interface.

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A very simple, one-dimensional, discrete, autonomous model of cell crawling is proposed; the model involves only three or four coupled first-order differential equations. This form is sufficient to describe many general features of cell migration, including both steady forward motion and oscillatory progress. Closed-form expressions for crawling speeds and internal forces are obtained in terms of dimensionless parameters that characterize active intracellular processes and the passive mechanical properties of the cell. Two versions of the model are described: a basic cell model with simple elastic coupling between front and rear, which exhibits stable, steady forward crawling after initial transient oscillations have decayed, and a poroelastic model, which can exhibit oscillatory crawling in the steady state.

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Actomyosin contractility spatiotemporally regulates actin network dynamics in migrating cells

Cited by 45 publications

References 40 publications

Cell Migration with Multiple Pseudopodia: Temporal and Spatial Sensing Models

Cell Migration with Multiple Pseudopodia: Temporal and Spatial Sensing Models

Myosin Light Chain Kinase (MLCK) Regulates Cell Migration in a Myosin Regulatory Light Chain Phosphorylation-independent Mechanism

Damped and persistent oscillations in a simple model of cell crawling

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