Actual proliferating index in oral squamous cell carcinoma and leukoplakia

Chandak, Abhay R.; Gadbail, Amol R.; Chaudhary, Minal; Chandak, Shweta A.; Wadhwani, Ritesh

doi:10.1111/j.2041-1626.2011.00057.x

Cited by 8 publications

(9 citation statements)

References 34 publications

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“…The median Ki-67 LI increased from keratosis without dysplasia to keratosis with dysplasia to OSCC in this study, and the relationship was statistically significant. As noted in this study, similar findings were observed in the studies performed by Iamaroon et al 16 , Dwivedi N et al 10 , Santos-Garcia A et al 17 and Dragomir LP et al 24 The median Ki-67 LI was also higher in keratosis with dysplasia than keratosis without dysplasia, concurrent with the findings by Kurokawa H et al 9 and Chandak AR et al 25 The increase in number of Ki-67 positive cells with the increase in histological grades of OSCC from WDSCC to MDSCC to PDSCC has been highlighted by various authors Ashraf MJ 7 , Verma R 20 , Dwivedi N et al 10 and Chandak AR et al 25 Moreover the studies done by Motta R et al 21 and Thomson PJ et al 23 have shown highest Ki-67 immunoexpression in lesion with cervical metastasis. The importance of Ki-67 overexpression in atypical oral epithelium and its association with recurrence/malignant transformation, independently of other established clinicopathological factors including age, lesion site, epithelial dysplasia has been highlighted by Yagyuu T et al in their study.…”

Section: Discussionsupporting

confidence: 91%

Role of p53 and Ki-67 immunomarkers in oral premalignant lesions and squamous cell carcinoma: a hospital based study in BPKIHS

et al. 2018

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Background: As most of the oral squamous cell carcinoma develop from precursor premalignant lesions, it would be helpful if the malignant transformation is detected early in premalignant state. The objective of the research was to study the role of immunohistochemical expression of p53 and Ki-67 in oral premalignant lesion and squamous cell carcinoma.Materials and Methods: The expression of immunomarkers p53 and Ki67 were studied on formalin fixed paraffin embedded tissue sections from human oral squamous mucosal lesion for duration of 1 year. Results: Of total 36 cases, 80% cases of keratosis without dysplasia showed basal pattern of p53 staining while 47.1% cases of squamous cell carcinoma showed p53 staining in all layers of epithelium. The median p53 Labelling Index of squamous cell carcinoma was more than those of keratosis with and without dysplasia though the result was statistically non-significant. 50.0% cases of keratosis without dysplasia and 83.3% cases of keratosis with dysplasia displayed Ki-67 immunostaining confined to basal and suprabasal layer whereas 94.1% cases of squamous cell carcinoma showed Ki-67 positivity in all layers of epithelium. Median Ki-67 Labelling Index increased from keratosis without dysplasia to keratosis with dysplasia to squamous cell carcinoma, difference being statistically significant. A positive and insignificant correlation was observed between p53 and Ki-67 Labelling Index.Conclusion: Increased expressions of Ki-67 and p53 in oral squamous cell carcinoma compared to premalignant lesion suggest that they may be useful indicator of malignant transformation in dysplastic lesion.

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Section: Discussionsupporting

confidence: 91%

Role of p53 and Ki-67 immunomarkers in oral premalignant lesions and squamous cell carcinoma: a hospital based study in BPKIHS

et al. 2018

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“…cases with negative margins developed a recurrence as compared t1 (30.4%) cases with close margins and 42.8%) cases with positive margins. According to Chandak et al [5] API in normal mucosa was found to be 7.41, in Leukoplakia -52.51. In OSCC-180.9.…”

Section: Discussionmentioning

confidence: 91%

“…API can distinguish between dysplastic and nondysplastic leukoplakia. So, it can be useful to evaluate dysplasia in surgical margins [5].…”

Section: Discussionmentioning

confidence: 99%

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Assessing the Utility of Actual Proliferation Index Measured by Expression of ‘Agnor and Ki-67’ in Histopathologically Negative Surgical Margins of Oral Squamous Cell Carcinoma as a Modality for Prediction of Recurrence

Gawande

Chaudhary

2021

JPRI

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Background: Oral squamous cell carcinoma is most common malignancy of head and neck which is the major cause of death. Recurrence is the major important prognostic factor in the survival of patients and tumor stage, degree of differentiation of neoplastic cells, pN stage resected margins, and lymphovascular invasion are the factors for recurrence. The rate of recurrence varies from 18 to76% for patients who underwent standard treatment. Histological assessment of surgical margins has a major role to predict the recurrence. But in spite of negative histological surgical margins recurrence rate is high, as there are genetic alterations in these margins which are not detected by routine histopathlogy. Even when surgical margins are free histopathologically the local recurrence rate is 10 to 30 percent. That’s why molecular evaluation of these margins should be done. Many immunohistochemical studies are done to assess surgical margins of OSCC but no study is carried out to assess the proliferative index in surgical margins. Therefore this study will help to improve management and predict the recurrence of OSCC. Objectives: In this study we aim to assess the utility of “actual proliferation index” measured by expression of “Agnor” and “Ki-67” in histopathologically negative “surgical margins” of “oral sqaumous cell carcinoma” as a modality for prediction of recurrence. Methodology: The resected surgical margins of the oral squamous cell carcinoma cases which are operated in Sharad Pawar dental college will be selected as samples. The anterior, posterior, inferior and superior margin of resected specimens will be stained by H&E staining. Immunohistochemical staining by ki-67 antibody will be done in the negative histopathological surgical margins. The same sections will be stained with AgNOR staining. Both sections will be evaluated. Actual proliferative index will be calculated with the given formula Ki67 Labeling Index: Ki67 L1= “Number of Ki67 positive cells” x 100 Total number of tumor cells observed “Actual Proliferative Index (PI)” = “ Ki-67” or MIB-1 LI × AgNOR count ’ Recurrence of tumor will be observed during first two years period after surgical procedure. Expected Results: The “actual proliferative index” calculated by “Ki-67 labelling index” and “AgNOR count expression in histopathologically” negative surgical margins of OSCC will be more in recurrence patients as compared to patients who do not have recurrence. Conclusion: The API values measured by assessing the Agnor and Ki67 expression, in histopathological negative surgical margins of OSCC will be compared between with values of patient with recurrence.

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“…Oral squamous cell carcinoma (OSCC) is one of the top ten cancers in the world,[ 2 ] ranks first among males and third among females in India. [ 3 ] Regardless of advanced diagnosis and treatment modalities, it carries potentially poor prognosis with an estimated survival rate of only 56%. [ 4 ] Prognosis of OSCC is intimately related to nodal metastasis.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical analysis of stromal fibrocytes and myofibroblasts to envision the invasion and lymph node metastasis in oral squamous cell carcinoma

Rao¹,

Rao

2017

J Oral Maxillofac Pathol

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Background:Tumor cells work in close coordination with stromal elements from its stage of emergence to metastasis. The study was designed to assess the presence and distribution pattern of stromal fibrocytes and myofibroblasts in oral squamous cell carcinoma (OSCC). Possibility of using these stromal cells as a marker for invasion and lymphnode metastasis was evaluated.Materials and Methods:A total of 40 cases of OSCC consisting twenty cases of each lymph node positive (pN+) and lymph node negative (pN0) samples and ten normal oral mucosa (NOM) tissues were subjected to double immunostaining using CD34 and alpha-smooth muscle actin (α-SMA) antibodies. Stained sections were evaluated semiquantitatively.Results:CD34 fibrocytes were seen in 70% of NOM and none of OSCC samples. α-SMA myofibroblasts were seen in 80% of OSCC and none of NOM samples. A statistically significant difference was found in fibrocyte values (P < 0.001) and myofibroblast values (P < 0.001) between NOM and OSCC study samples. No statistical significance in myofibroblast values between pN0 and pN+ study groups; however, their distribution pattern appreciably varied.Conclusions:This study suggested that fibrocytes could be used as one of the markers for early invasion. Abrupt loss of fibrocytes at the transition zone toward carcinoma and statistical significance in their values supported this inference. Heterogeneity in the distribution pattern of myofibroblasts in tumor stroma indicates that this variability may predict the tumor behavior toward nodal metastasis rather than their mere presence or absence.

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Actual proliferating index in oral squamous cell carcinoma and leukoplakia

Cited by 8 publications

References 34 publications

Role of p53 and Ki-67 immunomarkers in oral premalignant lesions and squamous cell carcinoma: a hospital based study in BPKIHS

Role of p53 and Ki-67 immunomarkers in oral premalignant lesions and squamous cell carcinoma: a hospital based study in BPKIHS

Assessing the Utility of Actual Proliferation Index Measured by Expression of ‘Agnor and Ki-67’ in Histopathologically Negative Surgical Margins of Oral Squamous Cell Carcinoma as a Modality for Prediction of Recurrence

Immunohistochemical analysis of stromal fibrocytes and myofibroblasts to envision the invasion and lymph node metastasis in oral squamous cell carcinoma

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