Acupuncture of the Beishu acupoint participates in regulatory effects of ginsenoside Rg1 on T cell subsets of rats with chronic fatigue syndrome

Abstract

Background: There are close relationships between the spleen and limb muscles and thoughts. The study aims to test the effects of ginsenoside Rg1 in combination with acupuncture of the Beishu acupoint on T cell subsets of rats with chronic fatigue syndrome (CFS). Methods:The model was set up by combining forced cold-water swimming with chronic restraint. The rats were randomly divided into blank control, model, ginsenoside, acupuncture, and ginsenoside plus acupuncture groups (n=10). For the acupuncture group, the Beishu acupoint was acupunctured on the 2nd day after modeling. For the ginsenoside group, the ginsenoside Rg1 solution was injected into the tail vein on the 2nd day after modeling. For the combination group, both processes were conducted. These groups were compared regarding exhausted swimming time, number of struggles, resting time, serum levels of IgA, IgG, IgM, IFN-α, IFN-β, and IFN-γ, lymphocyte transformation rate, T cell subsets, and skeletal muscle activities of malondialdehyde (MDA), total antioxidative capacity (T-AOC) and acetylcholinesterase (Ache). Results:The exhausted swimming time, number of struggles, and resting time of combination group surpassed those in the ginsenoside and acupuncture groups significantly (P<0.05). The serum levels of IgA, IgG, IgM, IFN-β, IFN-γ, T-AOC, and Ache, together with CD3 + and CD8 + T cell percentages of combination groups, were significantly higher than those of ginsenoside and acupuncture groups. However, the IFN-α level, MDA activity, and CD4 + T cell percentage were significantly lower (P<0.05). Compared with the model group, the CD4 + /CD8 + T cell ratios of acupuncture, ginsenoside, and combination groups decreased significantly (P<0.05). Compared with the combination group, the ratio of the ginsenoside group increased significantly (P<0.05).Conclusions: Both acupuncture of the Beishu acupoint and intravenous injection of ginsenoside Rg1 have anti-fatigue effects, and their combination works synergistically. This study supplies an experimental basis for joint therapy using acupuncture and drugs to combat fatigue synergistically.

Saireito (114) Increases IC50 and Changes T-Cell Phenotype When Used in Combination with Prednisolone Therapy in Human Peripheral Blood Mononuclear Cells

“…ere is a concern that macromolecules that include 114 might stimulate the proliferation of CD8 + T cells in autoimmune diseases, similar to how ginsenoside Rg1 included in ginseng (a component of 114) has been reported to increase the ratio of CD8 + T cells in vivo [23]. In contrast, the results of this study showed that 114 did not affect the increase in the ratio of CD8 + T cells when used in combination with PSL, suggesting that 300 μg/mL 114 is a safe dosage that does not elevate the ratio of CD8 + T cells when used in combination with PSL in vitro.…”

Frailty Improvement by Multicomponent Drug, Ninjin’Yoeito, in Mild Cognitive Impairment and Mild Alzheimer’s Disease Patients: An Open-Label Exploratory Study (FRAMINGO)

“…Ginseng, a component of NYT, is widely used for treating fatigue because it has been reported to improve energy, physical and emotional health, and well-being [ 44 ]. Ginsenoside Rg1, the main active ingredient in Ginseng, has been reported to have anti-fatigue effects by regulating the number of T cell subsets and enhancing immunity in rats with chronic fatigue syndrome [ 45 ]. Furthermore, one of the constituents of NYT, Schisandra Fruit, has been reported to improve endurance and energy metabolism in exercised rats and has the potential to upregulate peroxisome proliferator-activated receptor-gamma coactivator-1 α in skeletal muscle, which is a key regulator of energy metabolism [ 46 ].…”

Safety and efficacy of Ninjin’yoeito along with iron supplementation therapy for preoperative anemia, fatigue, and anxiety in patients with gynecological disease: an open-label, single-center, randomized phase-II trial

“…NYT has been suggested to improve the appetite via dopamine and NPY pathway [ 51 – 53 ] and to inhibit corticosterone by inhibiting NO synthesis [ 30 ]. In addition, schisandra fruit and citrus unshiu peel have been reported to have anti-anxiety effects [ 28 , 54 , 55 ] and ginseng has been reported to have anti-anxiety and anti-fatigue effects [ 56 , 57 ]. All of these are ingredients of NYT.…”