Acute Administration of Liposomal Coenzyme Q10Increases Myocardial Tissue Levels and Improves Tolerance to Ischemia Reperfusion Injury

Niibori, Koki; Yokoyama, Hitoshi; Crestanello, Juan A.; Whitman, Glenn J.R.

doi:10.1006/jsre.1998.5411

Cited by 29 publications

(25 citation statements)

References 23 publications

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“…2) the λ max values were 211 and 275 nm respectively, close to those obtained with CoQ 10 ethanol solution. It was also found that the A 275 nm of blank liposome solubilised with Tween 80 was 0.033, which would not interfere with the A 275 nm of CoQ 10 .…”

Section: Results and Discussion Coq 10 Liposome Solubilising Propertimentioning

confidence: 93%

“…Tween 80 ethanol solution (10 mL, 100 mg mL −1 ) was added to solubilise the CoQ 10 . The flask contents were then made up to 100 mL with deionised water and stored at 4 • C. Duplicate sets of CoQ 10 standard solution (0.25, 0.5, 1, 2, 3 and 4 mL, 119.4 µg mL −1 ) were added to 10 mL graduated flasks and diluted to 10 mL with 10 mg mL −1 Tween 80 solution.…”

Section: Uv Spectrophotometrymentioning

confidence: 99%

“…All samples were kept at 40 • C for 30 min. The CoQ 10 concentration was detected spectrophotometrically by A at 275 nm before and after reducing the CoQ 10 . With CoQ 10 concentration (C) as abscissa and A as ordinate, a curve was plotted and the regression equation obtained was A = 0.014C + 0.0102 (r 2 = 0.9995).…”

Section: Uv Spectrophotometrymentioning

confidence: 99%

“…7 -10 The liposomal delivery system may be a highly effective way of supplying a faster and longer-lasting dosage of this important nutrient to the systemic circulation for maximum effect. 7,10 Several methods have been developed for the measurement of CoQ 10 concentration, such as ultraviolet (UV) spectrophotometry 11 and high-performance liquid chromatography (HPLC) analysis. 12,13 However, CoQ 10 incorporated into a liposomal bilayer cannot be reduced by NaBH 4 during the determination process of UV spectrophotometry, or separated from the liposome membrane by HPLC column, and thus the amount of CoQ 10 actually incorporated into the liposomes cannot be measured directly.…”

Section: Introductionmentioning

confidence: 99%

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Improved assay of coenzyme Q₁₀ from liposomes by Tween 80 solubilisation and UV spectrophotometry

Xia

2006

J Sci Food Agric

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The encapsulated coenzyme Q 10 (CoQ 10 ) level is an important index in evaluating the quality of CoQ 10 liposomes. This study demonstrates a simple method to release encapsulated CoQ 10 from liposomal suspension using moderate amounts of the non-ionic surfactant Tween 80 to form mixed micelles containing phospholipids, Tween 80 and CoQ 10 . The encapsulated CoQ 10 level was detected by means of Tween 80 solubilisation and ultraviolet (UV) spectrophotometry, in which 1 mL of the reducing agent NaBH 4 (7 mg mL −1 ) was added. The proposed method had a good linear correlation with ethanol solubilisation and reverse phase high-performance liquid chromatography (RP-HPLC) and with ethanol solubilisation and UV spectrophotometry over a CoQ 10 concentration range of 2.5-50 µg mL −1 (R 2 > 0.999). The average recovery rate of CoQ 10 from blank liposomes was estimated as 99.46 ± 1.54%. The difference in results between the organic solvent extraction method and the Tween 80 solubilisation method was not statistically significant (P > 0.05). When the latter method was applied to determine the total and the encapsulated CoQ 10 content quantitatively, the relative standard deviation was lower than 5%. Therefore this method has proved to be convenient, sensitive, accurate and reproducible compared with conventional RP-HPLC analysis and UV spectrophotometry with organic solvent extraction.

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Section: Results and Discussion Coq 10 Liposome Solubilising Propertimentioning

confidence: 93%

Section: Uv Spectrophotometrymentioning

confidence: 99%

Section: Uv Spectrophotometrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Improved assay of coenzyme Q₁₀ from liposomes by Tween 80 solubilisation and UV spectrophotometry

Xia

2006

J Sci Food Agric

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“…Studies in vitro and animal experiments show that coenzyme Q10 can protect the cardiac muscle against functional and structural changes following ischaemia-reperfusion injury (Whitman et al 1997;Niborii et al 1998;Crestanello et al 2002).…”

Section: Coenzyme Q10 and Selected Pharmacological Aspectsmentioning

confidence: 99%

Coenzyme Q 10 – its biochemical and related aspects

et al. 2015

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This review analyses the findings of biochemical and related pharmacotherapeutical aspects of coenzyme Q10. Its important role in the respiratory chain is presented. Furthermore, the article presents administration of coenzyme Q10 as a supplement within preventative measures in medicine, its pharmacotherapeutical aspects and effects in a number of diseases of various aetiologies. Concurrently, it presents the issue of mutual interactions of coenzyme Q10 and its efficacy in combining supplementation with conservative therapy of selected aetiologies.

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Bioenergetic effect of liposomal coenzyme Q₁₀ on myocardial ischemia reperfusion injury

et al. 1999

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The antioxidant and bioenergetic effects of CoQ10 are well known but its clinical utility is limited by the requirement for enteral administration. A newly developed liposomal CoQ10 (CoQ) is water soluble and capable of intravenous administration. The purpose of this study is to determine the mechanism by which acute administration CoQ protects myocardium from reperfusion (Rp) injury. Rats were pretreated with CoQ 10 mg/kg i.v. 30 min prior to the experiment. Control rats were pretreated with liposome only. Hearts were excised and subjected to equilibration, 25 min of normothermic ischemia and 40 min of Rp on a Langendorff apparatus. At end Rp, CoQ hearts recovered 74 +/- 5% of their DP vs. 50 +/- 9% in control (p < 0.05). Aerobic efficiency was maintained (0.66 +/- 0.02 vs. control, 0.5 +/- 0.04, p < 0.003) and CoQ hearts lost less CK activity vs. control (p < 0.02). PCr and ATP were higher than control (p < 0.05, 0.02, respectively). Results show that i.v. CoQ improves recovery of function, aerobic efficiency, CK activity, and recovery of PCr and ATP after Rp. This suggests that acute administration of liposomal CoQ improves myocardial tolerance to I/R via its role as an antioxidant as well as improving oxygen utilization and high energy phosphate production.

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Acute Administration of Liposomal Coenzyme Q10Increases Myocardial Tissue Levels and Improves Tolerance to Ischemia Reperfusion Injury

Cited by 29 publications

References 23 publications

Improved assay of coenzyme Q₁₀ from liposomes by Tween 80 solubilisation and UV spectrophotometry

Improved assay of coenzyme Q₁₀ from liposomes by Tween 80 solubilisation and UV spectrophotometry

Coenzyme Q 10 – its biochemical and related aspects

Bioenergetic effect of liposomal coenzyme Q₁₀ on myocardial ischemia reperfusion injury

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Acute Administration of Liposomal Coenzyme Q10Increases Myocardial Tissue Levels and Improves Tolerance to Ischemia Reperfusion Injury

Cited by 29 publications

References 23 publications

Improved assay of coenzyme Q10 from liposomes by Tween 80 solubilisation and UV spectrophotometry

Improved assay of coenzyme Q10 from liposomes by Tween 80 solubilisation and UV spectrophotometry

Coenzyme Q 10 – its biochemical and related aspects

Bioenergetic effect of liposomal coenzyme Q10 on myocardial ischemia reperfusion injury

Contact Info

Product

Resources

About

Improved assay of coenzyme Q₁₀ from liposomes by Tween 80 solubilisation and UV spectrophotometry

Improved assay of coenzyme Q₁₀ from liposomes by Tween 80 solubilisation and UV spectrophotometry

Bioenergetic effect of liposomal coenzyme Q₁₀ on myocardial ischemia reperfusion injury