Acute administration of vitamin C abrogates protection from ischemic preconditioning in rabbits

Tsovolas, Konstantinos; Iliodromitis, Efstathios K.; Andreadou, Ioanna; Ζώγα, Αναστασία; Demopoulou, Maritina; Iliodromitis, Konstantinos; Manolaki, Theodora; Markantonis, Sophia L.; Kremastinos, Dimitrios Th.

doi:10.1016/j.phrs.2008.02.003

Cited by 23 publications

(20 citation statements)

References 41 publications

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“…Maintaining the suture loosely around the vessel at the site of occlusion facilitates re-occlusion at different time points and for post-mortem staining. Successfully reperfused myocardium is distinguished by the recovery of the color on the ventricular surface; however, bruising of the myocardium can also be observed as a result of hemoglobin degradation [220, 427]. In case the protocol requires recovery of the animals after surgery for further monitoring, the chest retractor is removed, the intercostal space is closed by intermittent ligatures using 2.0 sutures and the muscle layers are sutured together with 2.0 silk sutures [128].…”

Section: Small Animal Hearts In Situmentioning

confidence: 99%

Practical guidelines for rigor and reproducibility in preclinical and clinical studies on cardioprotection

et al. 2018

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Section: Small Animal Hearts In Situmentioning

confidence: 99%

Practical guidelines for rigor and reproducibility in preclinical and clinical studies on cardioprotection

et al. 2018

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“…However, available data are few and more large-scale clinical trials are still lacking to confirm this beneficial effect of vitamin C (Rasoli et al, 2011). It is noteworthy that vitamin C could also abrogate the beneficial effects of ischemic preconditioning, a phenomenon induced by a series of brief sub-lethal episodes of ischemia and reperfusion prior to a potentially lethal episode of ischemia that renders the heart more resistant to myocardial infarction (Tsovolas et al, 2008). Likely, the preconditioning is abolished due to an abrogation of ROS production, a phenomenon that otherwise could give rise to a survival response by the antioxidant defense system.…”

Section: Prevention Of Postoperative Atrial Fibrillationmentioning

confidence: 99%

Prevention of Postoperative Atrial Fibrillation: Novel and Safe Strategy Based on the Modulation of the Antioxidant System

Rodrigo¹

2012

Front. Physio.

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Postoperative atrial fibrillation (AF) is the most common arrhythmia following cardiac surgery with extracorporeal circulation. The pathogenesis of postoperative AF is multifactorial. Oxidative stress, caused by the unavoidable ischemia–reperfusion event occurring in this setting, is a major contributory factor. Reactive oxygen species (ROS)-derived effects could result in lipid peroxidation, protein carbonylation, or DNA oxidation of cardiac tissue, thus leading to functional and structural myocardial remodeling. The vulnerability of myocardial tissue to the oxidative challenge is also dependent on the activity of the antioxidant system. High ROS levels, overwhelming this system, should result in deleterious cellular effects, such as the induction of necrosis, apoptosis, or autophagy. Nevertheless, tissue exposure to low to moderate ROS levels could trigger a survival response with a trend to reinforce the antioxidant defense system. Administration of n−3 polyunsaturated fatty acids (PUFA), known to involve a moderate ROS production, is consistent with a diminished vulnerability to the development of postoperative AF. Accordingly, supplementation of n−3 PUFA successfully reduced the incidence of postoperative AF after coronary bypass grafting. This response is due to an up-regulation of antioxidant enzymes, as shown in experimental models. In turn, non-enzymatic antioxidant reinforcement through vitamin C administration prior to cardiac surgery has also reduced the postoperative AF incidence. Therefore, it should be expected that a mixed therapy result in an improvement of the cardioprotective effect by modulating both components of the antioxidant system. We present novel available evidence supporting the hypothesis of an effective prevention of postoperative AF including a two-step therapeutic strategy: n−3 PUFA followed by vitamin C supplementation to patients scheduled for cardiac surgery with extracorporeal circulation. The present study should encourage the design of clinical trials aimed to test the efficacy of this strategy to offer new therapeutic opportunities to patients challenged by ischemia–reperfusion events not solely in heart, but also in other organs such as kidney or liver in transplantation surgeries.

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“…3A) coupled with significantly better recovery of contraction and relaxation rates (Fig. 4A,B), while coronary flow was restored to the preischemic values ( Preservation of physiological ROS production with regards to metabolism and cell signaling, is the advantage of molecular hydrogen over other antioxidants (vitamin C, tetrakis-4-sulphonatophenyl-porphyrinato iron) which may blunt antiinfarct and antistunning effects of pre-or postconditioning (Li et al 2013;Tsolvas et al 2008). Therefore, hydrogen treatment may have a potential to be applied in a clinical practice to improve prognosis of the patients in reconvalescence after infarction or larger cardiosurgical interventions using heart-lung apparatus as well as after heart transplantation.…”

Section: Discussionmentioning

confidence: 98%

Molecular hydrogen potentiates beneficial anti-infarct effect of hypoxic postconditioning in isolated rat hearts: a novel cardioprotective intervention

Zálešák

Kura

Graban

et al. 2017

Can. J. Physiol. Pharmacol.

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Keyword:Ischemia-reperfusion injury, ischemic postconditioning, hypoxic postconditioning, molecular hydrogen, antioxidants https://mc06.manuscriptcentral.com/cjpp-pubs AbstractGeneration of free radicals through incomplete reduction of oxygen during ischemia/ reperfusion is well-described. On the other hand, molecular hydrogen (H 2 ) reduces oxidative stress due to its ability to react with strong oxidants and easily penetrate cells by diffusion, without disturbing metabolic redox reactions. This study was designed to explore cardioprotective potential of hypoxic postconditioning (HpostC) against ischemia/reperfusion (30-min global I/120-min R) in isolated rat hearts using oxygen-free Krebs-Henseleit buffer (KHB). Furthermore, the possibility to potentiate the effect of HpostC by H 2 using oxygenfree KHB saturated with H 2 (H2+HpostC) was tested. HPostC was induced by 4 cycles of 1-min perfusion with oxygen-free KHB intercepted by 1-min perfusion with normal KHB, at the onset of reperfusion. H 2 +HPostC was applied in a similar manner using H 2 enriched oxygen-free KHB. Cardioprotective effects were evaluated on the basis of infarct size (IS, in % of area at risk, AR) reduction, post-I/R recovery of heart function and occurrence of reperfusion arrhythmias. HPostC significantly reduced IS/AR compared to non-conditioned controls. H 2 present in KHB during HPostC further decreased IS/AR compared to the effect of HPostC, attenuated severe arrhythmias, and significantly restored heart function (vs. controls). Cardioprotection by hypoxic postconditioning can be augmented by molecular hydrogen infusion.

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Acute administration of vitamin C abrogates protection from ischemic preconditioning in rabbits

Cited by 23 publications

References 41 publications

Practical guidelines for rigor and reproducibility in preclinical and clinical studies on cardioprotection

Practical guidelines for rigor and reproducibility in preclinical and clinical studies on cardioprotection

Prevention of Postoperative Atrial Fibrillation: Novel and Safe Strategy Based on the Modulation of the Antioxidant System

Molecular hydrogen potentiates beneficial anti-infarct effect of hypoxic postconditioning in isolated rat hearts: a novel cardioprotective intervention

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