Acute alcohol induces greater dose-dependent increase in the lateral cortical network functional connectivity in adult than adolescent rats

Lee, Sung-Ho; Shnitko, Tatiana A.; Hsu, Li-Ming; Broadwater, Margaret A.; Sardinas, Mabelle; Wang, Tzu-Wen Winnie; Robinson, Donita L.; Vetreno, Ryan P.; Crews, Fulton T.; Shih, Yen-Yu Ian

doi:10.1016/j.addicn.2023.100105

Cited by 6 publications

(2 citation statements)

References 118 publications

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“…fMRI Data collection. The fMRI scans were conducted one week after the circling tests following the procedures established in several recent studies 15,[92][93][94] . Briefly, rats were intubated and ventilated under dexmedetomidine (0.025mg/kg/hour, i.p infusion) and 0.5% isoflurane.…”

Section: Methodsmentioning

confidence: 99%

Optogenetic fMRI reveals therapeutic circuits of subthalamic nucleus deep brain stimulation

Li,

Lee,

et al. 2024

Preprint

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SummaryWhile deep brain stimulation (DBS) is widely employed for managing motor symptoms in Parkinson’s disease (PD), its exact circuit mechanisms remain controversial. To identify the neural targets affected by therapeutic DBS in PD, we analyzed DBS-evoked whole brain activity in female hemi-parkinsonian rats using function magnetic resonance imaging (fMRI). We delivered subthalamic nucleus (STN) DBS at various stimulation pulse repetition rates using optogenetics, allowing unbiased examinations of cell-type specific STN feed-forward neural activity. Unilateral STN optogenetic stimulation elicited pulse repetition rate-dependent alterations of blood-oxygenation-level-dependent (BOLD) signals in SNr (substantia nigra pars reticulata), GP (globus pallidus), and CPu (caudate putamen). Notably, these manipulations effectively ameliorated pathological circling behavior in animals expressing the kinetically faster Chronos opsin, but not in animals expressing ChR2. Furthermore, mediation analysis revealed that the pulse repetition rate-dependent behavioral rescue was significantly mediated by optogenetically induced activity changes in GP and CPu, but not in SNr. This suggests that the activation of GP and CPu are critically involved in the therapeutic mechanisms of STN DBS.

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Section: Methodsmentioning

confidence: 99%

Optogenetic fMRI reveals therapeutic circuits of subthalamic nucleus deep brain stimulation

Li,

Lee,

et al. 2024

Preprint

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“…At present, the worldwide consumption of all kinds of alcohol and alcoholic beverages is increasing [ 1 , 2 ]. Moderate drinking can promote digestion, eliminate fatigue, stabilize blood sugar, and improve sleep quality [ 3 ]. However, excessive drinking can lead to alcoholism, causing headaches, dizziness, vomiting, and other uncomfortable symptoms.…”

Section: Introductionmentioning

confidence: 99%

Preparation, Structural Identification, and Screening of Egg-Derived Peptides with Facilitating Alcohol Metabolism Activity

Tan,

Wang,

Wan

et al. 2024

Foods

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The aim of this study was to obtain egg-derived peptides with facilitating alcohol metabolism (EPs) by enzymolysis, to identify their structures, and screen small polypeptides with higher activity by molecular docking. The optimum conditions for preparing EPs with facilitating alcohol metabolism were obtained by a single factor experiment, adding 2% Protamex and performing enzymolysis for 3 h with a liquid–material ratio of 35:1. The dose–response relationship experiment showed that 800 mg/kg·bw EPs played a better role in facilitating alcohol metabolism. EPs contained 40% hydrophobic amino acids (HAA), including 9.24% Leu. Eighty-four peptides were identified by HPLC-MS/MS and four peptides with potential activation of alcohol dehydrogenase were further selected by molecular docking. The tetrapeptide Trp-Ile-Val-Asp (WIVD) with the highest binding energy reached −7.16 kcal/mol. These findings suggest that egg is a good source for the preparation of peptides with facilitating alcohol metabolism activity.

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Neuromodulation in Small AnimalfMRI

Hsu,

Shih

2024

Magnetic Resonance Imaging

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The integration of functional magnetic resonance imaging (fMRI) with advanced neuroscience technologies in experimental small animal models offers a unique path to interrogate the causal relationships between regional brain activity and brain‐wide network measures—a goal challenging to accomplish in human subjects. This review traces the historical development of the neuromodulation techniques commonly used in rodents, such as electrical deep brain stimulation, optogenetics, and chemogenetics, and focuses on their application with fMRI. We discuss their advantageousness roles in uncovering the signaling architecture within the brain and the methodological considerations necessary when conducting these experiments. By presenting several rodent‐based case studies, we aim to demonstrate the potential of the multimodal neuromodulation approach in shedding light on neurovascular coupling, the neural basis of brain network functions, and their connections to behaviors. Key findings highlight the cell‐type and circuit‐specific modulation of brain‐wide activity patterns and their behavioral correlates. We also discuss several future directions and feature the use of mediation and moderation analytical models beyond the intuitive evoked response mapping, to better leverage the rich information available in fMRI data with neuromodulation. Using fMRI alongside neuromodulation techniques provide insights into the mesoscopic (relating to the intermediate scale between single neurons and large‐scale brain networks) and macroscopic fMRI measures that correlate with specific neuronal events. This integration bridges the gap between different scales of neuroscience research, facilitating the exploration and testing of novel therapeutic strategies aimed at altering network‐mediated behaviors. In conclusion, the combination of fMRI with neuromodulation techniques provides crucial insights into mesoscopic and macroscopic brain dynamics, advancing our understanding of brain function in health and disease.Evidence Level1Technical EfficacyStage 1

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Acute alcohol induces greater dose-dependent increase in the lateral cortical network functional connectivity in adult than adolescent rats

Cited by 6 publications

References 118 publications

Optogenetic fMRI reveals therapeutic circuits of subthalamic nucleus deep brain stimulation

Optogenetic fMRI reveals therapeutic circuits of subthalamic nucleus deep brain stimulation

Preparation, Structural Identification, and Screening of Egg-Derived Peptides with Facilitating Alcohol Metabolism Activity

Neuromodulation in Small AnimalfMRI

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