Acute and Chronic Exposure to 900 MHz Radio Frequency Radiation Activates p38/JNK-mediated MAPK Pathway in Rat Testis

Er, Hakan; Tas, Gizem Gamze; Soygur, Bikem; Özen, Şükrü; Satı, Leyla

doi:10.1007/s43032-022-00844-y

Cited by 7 publications

(17 citation statements)

References 86 publications

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“…The KEGG analysis showed that the significantly enriched pathways after treatment with carbon ions of different LET were similar (; https://doi.org/10.1667/RADE-23-00097.1.S1); among them, cellular senescence and MAPK signaling pathways were both significantly, and this has been found to be related to tumorigenesis and cancer development ( 36 , 37 ). Previous studies have found that FGFR and P38 could affect the MAPK signaling pathway to regulate radiosensitivity ( 47 , 48 ). In our study, we have made a novel discovery involving the gene GNG12.…”

Section: Discussionmentioning

confidence: 99%

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Remodeling of Chromatin Accessibility Regulates the Radiological Responses of NSCLC A549 Cells to High-LET Carbon Ions

Li,

Wang,

et al. 2023

Radiation Research

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Carbon-ion radiation therapy (CIRT) may offer remarkable advantages in cancer treatment with its unique physical and biological characteristics. However, the underlying epigenetic regulatory mechanisms of cancer response to CIRT remain to be identified. In this study, we showed consistent but different degrees of biological effects induced in NSCLC A549 cells by carbon ions of different LET. The genome-wide chromatin accessibility and transcriptional profiles of carbon ion-treated A549 cells were performed using transposase-accessible chromatin sequencing (ATAC-seq) and RNA-seq, respectively, and further gene regulatory network analysis was performed by integrating the two sets of genomic data. Alterations in chromatin accessibility by carbon ions of different LET predominantly occurred in intron, distal intergenic and promoter regions of differential chromatin accessibility regions. The transcriptional changes were mainly regulated by proximal chromatin accessibility. Notably, CCCTC-binding factor (CTCF) was identified as a key transcription factor in the cellular response to carbon ions. The target genes regulated by CTCF in response to carbon ions were found to be closely associated with the LET of carbon ions, particularly in the regulation of gene transcription within the DNA replication- and metabolism-related signaling pathways. This study provides a regulatory profile of genes involved in key signaling pathways and highlighted key regulatory elements in NSCLC A549 cells during CIRT, which expands our understanding of the epigenetic mechanisms of carbon ion-induced biological effects and reveals an important role for LET in the regulation of changes in chromatin accessibility, although further research is needed.

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Section: Discussionmentioning

confidence: 99%

“…MAPK signaling pathway to regulate radiosensitivity (47,48). In our study, we have made a novel discovery involving the gene GNG12.…”

Section: Chromatin Remodeling Drives Nsclc Response To Carbon Ionsmentioning

confidence: 99%

Remodeling of Chromatin Accessibility Regulates the Radiological Responses of NSCLC A549 Cells to High-LET Carbon Ions

Li,

Wang,

et al. 2023

Radiation Research

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“…Except for the 2100 MHz RFR frequency and the distance of the rats from the antenna, the details of the experimental setup and RFR application were as previously reported [17]. An RF generator which produce 2100 MHz RFR (2100 MHz UMTS Simulator; Everest Corp., Adapazari, Türkiye) was used to represent the exposure of the UMTS.…”

Section: Radiofrequency Radiation Treatmentmentioning

confidence: 99%

“…Our research group have recently reported that p38/JNKmediated MAPK signaling was triggered significantly in the rat testis after 900 MHz RFR exposure [17]. However, it is unknown whether the p38/JNK-mediated MAPK signaling is also involved in the rat testis after 2100 MHz RFR exposure, which is used in global mobile communication systems (GSM).…”

Section: Introductionmentioning

confidence: 99%

Stress-responsive MAPK signaling pathway with proliferation and apoptosis in the rat testis after 2100 MHz radiofrequency radiation exposure

Er,

Tas,

Soygur

et al. 2024

Eur J Ther

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Objective: Mobile phone technology has progressed quickly in recent years. Cell phones operate using radiofrequency radiation (RFR), and the complete biological impacts of RFR remain unidentified. Thus, we aimed to investigate the potential effects of 2100 MHz radiofrequency radiation exposure on the stress-responsive JNK/p38 MAPK pathway, apoptosis and proliferation in rat testis. Methods: RFR groups were created with 2100 MHz RFR exposure for acute (2 h/day for 1 week) and chronic (2 h/day for 10 weeks) periods. Sham groups were kept under identical conditions without RFR. The cell apoptosis and histopathological changes in testis were evaluated. Immunolocalization of PCNA, active caspase-3, Bcl-xL, p-JNK and p-p38 were analyzed by immunohistochemistry, the total protein expressions were identified by Western blot. Results: There were no differences between RFR and sham groups by means of histopathology and TUNEL analysis. Also, the expression levels and the immunolocalization patterns of PCNA, active caspase-3 and Bcl-xL proteins were not altered. p-JNK and p-p38 protein expressions were prominently elevated in acute and chronic RFR groups. Conclusion: In conclusion, 2100 MHz RFR exposure had no considerably deleterious consequences on cellular proliferation and apoptosis processes in rat testis. However, increased expression of stress-activated protein kinases, p-JNK and p-p38, suggests the involvement of the MAPK signaling pathway as a critical (may be detrimental) cellular response.

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“…Further, DNA fragmentation has been found to increase following exposure to 850 MHz microwave [ 55 ], and was supported by an additional study where DNA fragmentation increased after exposure to 900–1800 MHz microwave radiation [ 56 ]. An in vivo study reported that 900 MHz radio frequency radiation activated the p38/JNK-mediated mitogen-activated protein kinase (MAPK) pathway in rat testes [ 57 ]. Further, there is strong evidence that the negativity or neutrality of microwave radiation effects on reproductive systems are dependent upon frequency and power.…”

Section: Biological Effects Of Microwavesmentioning

confidence: 99%

Microwave Radiation and the Brain: Mechanisms, Current Status, and Future Prospects

Mumtaz

Rana

Choi

et al. 2022

IJMS

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Modern humanity wades daily through various radiations, resulting in frequent exposure and causing potentially important biological effects. Among them, the brain is the organ most sensitive to electromagnetic radiation (EMR) exposure. Despite numerous correlated studies, critical unknowns surround the different parameters used, including operational frequency, power density (i.e., energy dose), and irradiation time that could permit reproducibility and comparability between analyses. Furthermore, the interactions of EMR with biological systems and its precise mechanisms remain poorly characterized. In this review, recent approaches examining the effects of microwave radiations on the brain, specifically learning and memory capabilities, as well as the mechanisms of brain dysfunction with exposure as reported in the literature, are analyzed and interpreted to provide prospective views for future research directed at this important and novel medical technology for developing preventive and therapeutic strategies on brain degeneration caused by microwave radiation. Additionally, the interactions of microwaves with biological systems and possible mechanisms are presented in this review. Treatment with natural products and safe techniques to reduce harm to organs have become essential components of daily life, and some promising techniques to treat cancers and their radioprotective effects are summarized as well. This review can serve as a platform for researchers to understand the mechanism and interactions of microwave radiation with biological systems, the present scenario, and prospects for future studies on the effect of microwaves on the brain.

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Acute and Chronic Exposure to 900 MHz Radio Frequency Radiation Activates p38/JNK-mediated MAPK Pathway in Rat Testis

Cited by 7 publications

References 86 publications

Remodeling of Chromatin Accessibility Regulates the Radiological Responses of NSCLC A549 Cells to High-LET Carbon Ions

Remodeling of Chromatin Accessibility Regulates the Radiological Responses of NSCLC A549 Cells to High-LET Carbon Ions

Stress-responsive MAPK signaling pathway with proliferation and apoptosis in the rat testis after 2100 MHz radiofrequency radiation exposure

Microwave Radiation and the Brain: Mechanisms, Current Status, and Future Prospects

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