Acute and long-term exposure to chlorpyrifos induces cell death of basal forebrain cholinergic neurons through AChE variants alteration

Pino, Javier del; Moyano, Paula; Anadón, M.J.; García, José M.; Díaz, María Jesús Villamide; Frejo, María Teresa

doi:10.1016/j.tox.2015.07.004

Cited by 20 publications

(7 citation statements)

References 76 publications

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“…1) are both located on a geographical line running East-West in Burkina Faso, approximately 30 Km south of the capital Ouagadougou; whereas most of the other villages, of which several showed over all negative results (Bani, You, Kindi, Zorgho), are located north of this line. Since the effect of E. alba extract in both villages (Diapangou and Ipendo) appeared to be positive and robust (20-40% yield increase, 17 and 24 field tests respectively, Fig.1), it could make sense to focus solely on the plant extract since the pesticide, Calthio C, is toxic to humans (Cereser et al, 2001;Pino et al, 2015) and protective measures for farmers using this agent in Burkina Faso are insufficient (Toe et al, 2013). In addition, the plant, E. alba, can easily be found near the two villages Diapangou and Ipendo (Zida et al, unpublished results).…”

Section: Factors Influencing the Effect Of Seed Treatmentmentioning

confidence: 99%

Effect of sorghum seed treatment in Burkina Faso varies with baseline crop performance and geographical location

Zida¹,

Néya²,

Soalla³

et al. 2016

Afr. Crop Sci. J.

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Section: Factors Influencing the Effect Of Seed Treatmentmentioning

confidence: 99%

Effect of sorghum seed treatment in Burkina Faso varies with baseline crop performance and geographical location

Zida¹,

Néya²,

Soalla³

et al. 2016

Afr. Crop Sci. J.

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“…Organophosphorus pesticides (OPs) are among the more commonly found pollutants in human body fluids. Although, OPs are known for their neurotoxic effects through inhibition of acetylcholinesterase (AChE) (Del Pino et al, 2015;Pallotta et al, 2017), recent evidence indicate that they also exert adverse effects upon the mammalian male reproductive system (Selvaraju et al, 2011;Dhanuskae and Peiris, 2017).…”

Section: Introductionmentioning

confidence: 99%

In vitro exposure to CPF affects bovine sperm epigenetic gene methylation pattern and the ability of sperm to support fertilization and embryo development

Pallotta

Barbato

Pinton

et al. 2018

Environ and Mol Mutagen

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Several studies have demonstrated that overexposure to pesticides can reduce mammalian sperm quality, impairing male fertility. Chlorpyrifos (CPF), a widely used organophosphate pesticide, was shown to impair spermatogenesis by inducing the formation of highly reactive toxic intermediates. To gain further insight into the mechanisms underlying the cytotoxicity and genotoxicity of CPF, bovine spermatozoa were exposed in vitro to environmental CPF concentrations and the motility, in vitro fertilization rates, DNA fragmentation, chromatin alterations, and methylation patterns were assessed. Motility and in vitro fertilization rates were significantly reduced in spermatozoa exposed to CPF, while DNA fragmentation and putative chromatin deconstruction appeared to increase at higher pesticide concentrations. In situ hybridization was carried out with X and Y probes on sperm samples exposed to different CPF concentrations, and subsequent analysis highlighted a significant percentage of spermatozoa with a peculiar morphological malformation, in which a narrowing occurred at the level of the hybridization. Analysis of potential abnormalities in the methylation pattern of NESP55‐GNAS and XIST promoters displayed no differentially methylated regions in GNAS promoter relative to the control, whereas spermatozoa exposed to 10 μg/mL CPF had increased methylation variance in one region of imprinted XIST promoter. Our results provide support that CPF can induce a genotoxic effect on spermatozoa, impairig their ability to fertilize and support preimplantation embryo development in vitro. These observations are worrying since altered levels of sporadic methylation in genes of male gametes may affect the success of reproduction and contribute to infertility. Environ. Mol. Mutagen. 60:85–95, 2019. © 2018 Wiley Periodicals, Inc.

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“…CPF is a known AchE inhibitor ( Yen et al, 2011 ; Reiss et al, 2012 ); no effect of CPF has been reported on nAchR activity in C. elegans . Additionally, CPF has also been shown to exhibit cholinergic neurodegeneration in SN56 basal forebrain cholinergic neurons ( del Pino et al, 2015 ) and alter cholinergic neurochemistry in developing rats. Similarly, CPF has been shown to exhibit detrimental effects on dopaminergic cells in vitro ( Lou and Li, 2016 ) and in young adult rats ( Zhang et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Caenorhabditis elegans Neurotoxicity Testing: Novel Applications in the Adverse Outcome Pathway Framework

et al. 2022

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Neurological hazard assessment of industrial and pesticidal chemicals demands a substantial amount of time and resources. Caenorhabditis elegans is an established model organism in developmental biology and neuroscience. It presents an ideal test system with relatively fewer neurons (302 in hermaphrodites) versus higher-order species, a transparent body, short lifespan, making it easier to perform neurotoxic assessment in a time and cost-effective manner. Yet, no regulatory testing guidelines have been developed for C. elegans in the field of developmental and adult neurotoxicity. Here, we describe a set of morphological and behavioral assessment protocols to examine neurotoxicity in C. elegans with relevance to cholinergic and dopaminergic systems. We discuss the homology of human genes and associated proteins in these two signaling pathways and evaluate the morphological and behavioral endpoints of C. elegans in the context of published adverse outcome pathways of neurodegenerative diseases. We conclude that C. elegans neurotoxicity testing will not only be instrumental to eliminating mammalian testing in neurological hazard assessment but also lead to new knowledge and mechanistic validation in the adverse outcome pathway framework.

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Acute and long-term exposure to chlorpyrifos induces cell death of basal forebrain cholinergic neurons through AChE variants alteration

Cited by 20 publications

References 76 publications

Effect of sorghum seed treatment in Burkina Faso varies with baseline crop performance and geographical location

Effect of sorghum seed treatment in Burkina Faso varies with baseline crop performance and geographical location

In vitro exposure to CPF affects bovine sperm epigenetic gene methylation pattern and the ability of sperm to support fertilization and embryo development

Caenorhabditis elegans Neurotoxicity Testing: Novel Applications in the Adverse Outcome Pathway Framework

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