Acute and Repeated Oral Toxicity of Antidiabetic Polyherbal Formulation Flax Seed, Fenugreek and Jamun Seeds in Wistar Albino Rat

Abstract

Polyherbal formulations treating for various diseases bring attention to the human society. The toxicity and its safe dosage should be studied for dose optimization and formulation. The aim of this study was to evaluate acute and repeated oral toxic effect of the hydroalcoholic extract of polyherbal formulation such as flax seed, fenugreek and jamun seed (FFJ) on wistar albino rats. Acute toxicity was tested by single oral stepwise administration of the drug at the dose of 300 mg, 2000 mg and 5000 mg/kg bw. The toxicity and mortality were observed for every 30 minutes for first 24 hours and then day by day for 14 days. At the 29th day, the blood was collected by Sino orbital puncture for analysis of hematological and biochemical parameters. Then the animals were killed by cervical decapitation and organs were separated and weighed. The results showed that no toxic symptoms were observed up to the dose of 2000 mg/kgbw. But slight changes in the stool consistency, tremor, lethargy and sleep were observed at the dose of 5000 mg/kg bw. There is no significant change in the biochemical and liver enzymatic parameters of different treated groups of polyherbal formulations. The control group animals was observed as

Acute and sub-chronic toxicity of Liberin, an anti-diabetic polyherbal formulation in rats

“…Blood is a non-invasive body fluid of choice which is directly under the control of haemopoietic system alterations, and it serves as the main transport medium for many drugs and xenobiotics in the body, also blood components are effortlessly exposed to substantial concentrations of toxic compounds. Circulating blood parameters are a true reflection of metabolomic and process derangements and serve as an excellent prognostic indicator for toxicity, therefore assessment of blood parameters is considered appropriate for drug toxicity risk assessment [ 26 ]. No changes were recorded in blood cell production or haematological parameters after treatment with PHF in this study, indicating that this drug did not adversely affect blood cells.…”