Acute and Sub-Acute Oral Toxicity Studies of Aqueous Extract of Securidaca Longepedunculata Fresen (Polygalaceae) Root Barks in Rodents.

Konate, Almamy; Ouedraogo, GeoffroyG.; Ilboudo, Sylvain; Ouédraogo, Noufou; Kaboré, Adama; Amadou, Traore; Guissou, InnocentP.; Tamboura, HamidouH.

doi:10.21474/ijar01/2429

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…Water and laboratory pellets, enriched with 29% protein, were freely accessible to the animals. The experimental protocol was carried out following international standard protocols [Guidelines set by the European Union on the protection of animals (CEC Council 86/609)] and adopted by IRSS, Burkina Faso [ 20 , 21 ]. All sections of this report adhere to the ARRIVE guidelines for reporting animal research ( Additional file 1 ).…”

Section: Methodsmentioning

confidence: 99%

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Toxicity and bacterial anti-motility activities of the hydroethanolic extract of Acacia senegal (L.) Willd (Fabaceae) leaves

Magnini

Nitiéma

Ouédraogo

et al. 2021

BMC Complement Med Ther

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Background Acacia senegal is a plant traditionally used for its various properties, including the treatment of infectious diseases. Recently, our team has demonstrated the ability of the hydroethanolic extract of the leaves to increase the activity of phenicol antibiotics against multi-resistant bacteria. The aim of this work is to determine the toxicological effects of the extract and its capacity to inhibit the bacterial mobility of Gram-negative bacteria, in order to evaluate the level of safety use of this plant. Methods The cytotoxicity test was performed using the neutral red absorption method. Acute and sub-acute oral toxicity were conducted on NMRI mice and Wistar rats. The behaviour and adverse effects were recorded during the 14 days of the acute study. For the subacute test, biochemical parameters, food and water consumption, and morphological parameters were determined. The anti-motility activities were evaluated on Pseudomonas aeruginosa PA01 and Escherichia coli AG100, using specific concentrations of Agar as required by the method. Results HEASG induced inhibition of keratinocytes cell growth with an IC50 of 1302 ± 60 μg/mL. For the acute toxicity study in mice, the single dose of extract of 2000 mg/kg body weight caused no deaths and no behavioural changes were observed; therefore, the median lethal dose (LD50) of HEASG was calculated to 5000 mg/kg body weight. In Wistar rats, no mortality was observed at 250, 500 and 1000 mg/kg/day during the 28-day subacute oral toxicity study. The weights of both females and males increased globally over time, regardless of the batch. No statistically significant differences were registered for organ weights and biochemical parameters, except for chloride for biochemical parameters. Water and food consumption did not change significantly. Furthermore, no macroscopic changes in organ appearance were observed. Regarding anti-motility activity, the extract has reduced the swarming motility of PA01 and AG100 significantly at the concentration of 32 μg/mL (P < 0.001). The extract has reduced the swimming motility (P < 0.01) of PA01 but not AG100. Conclusions The results suggest that hydroethanolic extract of A. senegal leaves has significant activity against bacterial motility and relatively low toxicity.

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Section: Methodsmentioning

confidence: 99%

“…Two hours after treatment, all animals were observed, and feeding was re-established. They are then observed at least once daily for 14 days for mortality and signs of toxicity such as changes in skin and fur, eyes, mucus membranes, salivation, convulsion, diarrhoea, lethargy, sleep, and coma [ 20 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

Toxicity and bacterial anti-motility activities of the hydroethanolic extract of Acacia senegal (L.) Willd (Fabaceae) leaves

Magnini

Nitiéma

Ouédraogo

et al. 2021

BMC Complement Med Ther

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“…The experimental protocol was carried out following to the international standard protocols [Guidelines set by the European Union on the Protection of Animals (CEC Council 86/609)] and adopted by IRSS, Burkina Faso (Ilboudo et al, 2019;Konaté et al, 2016).…”

Section: Animalsmentioning

confidence: 99%

Assessment of Oral Toxicity, Skin and Eye Irritation of Cymbopogon Nardus (L.) Rendle (Poaceae) Essential Oil From Burkina Faso

Ouédraogo¹,

Sawadogo²,

Koussé³

et al. 2020

IJAR

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Cymbopogon nardus (L.) Rendle (Poaceae) (C. nardus) still known as Ceylon citronella is an aromatic plant imported from India, Sri Lanka and introduced to the rest of the world including Africa. In Burkina Faso, the essential oil (EO) of this plant is used to formulate biopesticides. The objectives of this study consisted of evaluating the toxicological profile of the EO of C. nardus for its safe use. Acute and sub-acute oral toxicity was assessed on Wistar rats and skin, and eye irritant power was determined on rabbits according to the Organization for Economic Cooperation and Development (OECD) guidelines. In Acute oral toxicity test, a single administration of EO at a dose of 2000 mg/kg.b.w. to rats did not result in mortality or sign of toxicity. The LD50 of this EO is estimated to 5000 mg/kg b.w. The 28 days subacute toxicity study with doses of 100, 500 and 1000 mg/kg.b.w of EO did not show mortality in both sexes. Furthermore, there was no significant difference observed in body weight gain, relative organ weights, food and water consumption in test groups compared to control ones. However, there was a significant increase in AST, creatinine levels at a dose of 1000 mg/kg.b.w. and total protein at tested doses in both sexes. The skin and eye irritation studies on rabbit show that the EO had a very weak irritant power on the skin and the eye. These results suggested that C. nardus EO can constitute an alternative to synthetic pesticides and used in biopesticides formulation.

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Effects of the Pregnancy and Newborn Diagnostic Assessment (PANDA) App on Antenatal Care Quality in Burkina Faso: Protocol for a Cluster Randomized Controlled Trial (Preprint)

Coulibaly¹,

Kouanda²

2022

Preprint

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BACKGROUND The Pregnancy and Newborn Diagnostic Assessment (PANDA) system is a digital clinical decision support tool that can facilitate diagnosis and decision-making by health care personnel in antenatal care (ANC). Studies conducted in Madagascar and Burkina Faso showed that PANDA is a feasible system acceptable to various stakeholders. OBJECTIVE This study primarily aims to evaluate the effects of the PANDA system on ANC quality at rural health facilities in Burkina Faso. The secondary objectives of this study are to test the effects of the PANDA system on women’s satisfaction, women’s knowledge on birth preparedness and complication readiness, maternal and child health service use, men’s involvement in maternal health service utilization, and women’s contraception use at 6 weeks postpartum. Further, we will identify the factors that hinder or promote such an app and contribute to cost-effectiveness analysis. METHODS This is a randomized controlled trial implementing the PANDA system in 2 groups of health facilities (intervention and comparison groups) randomized using a matched-pair method. We included pregnant women who were <20 weeks pregnant during their first antenatal consultation in health facilities, and we followed up with them until their sixth week postpartum. Thirteen health centers were included, and 423 and 272 women were enrolled in the intervention and comparison groups, respectively. The primary outcome is a binary variable derived from the quality score, coded 1 (yes) for women with at least 75% of the total score and 0 if not. Data were collected electronically using tablets by directly interviewing the women and by extracting data from ANC registers, delivery registers, ANC cards, and health care records. The study procedures were standardized across all sites. We will compare unadjusted and adjusted primary outcome results (ANC quality scores) between the 2 study arms. We added a qualitative evaluation of the implementation of the PANDA system to identify barriers and catalysts. We also included an economic evaluation to determine whether the PANDA strategy is more cost-effective than the usual ANC strategy. RESULTS The enrollment ran from July 2020 to January 2021 due to the COVID-19 pandemic. Data collection ended in September 2022. Data analyses started in January 2023, ended in June 2023, and the results are expected to be published in February 2024. CONCLUSIONS The PANDA system is one of the most comprehensive apps for ANC because it has many features. However, the use of computerized systems for ANC is limited. Therefore, our trial will be beneficial for evaluating the intrinsic capacity of the PANDA system to improve the quality of care. By including qualitative research and economic evaluation, our findings will be significant because electronic consultation registries are expected to be used for maternal health care in the future in Burkina Faso. CLINICALTRIAL Pan-African Clinical Trials Registry (PACTR) PACTR202009861550402; https://pactr.samrc.ac.za/TrialDisplay.aspx?TrialID=12374 INTERNATIONAL REGISTERED REPORT DERR1-10.2196/37136

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Acute and Sub-Acute Oral Toxicity Studies of Aqueous Extract of Securidaca Longepedunculata Fresen (Polygalaceae) Root Barks in Rodents.

Cited by 4 publications

References 16 publications

Toxicity and bacterial anti-motility activities of the hydroethanolic extract of Acacia senegal (L.) Willd (Fabaceae) leaves

Toxicity and bacterial anti-motility activities of the hydroethanolic extract of Acacia senegal (L.) Willd (Fabaceae) leaves

Assessment of Oral Toxicity, Skin and Eye Irritation of Cymbopogon Nardus (L.) Rendle (Poaceae) Essential Oil From Burkina Faso

Effects of the Pregnancy and Newborn Diagnostic Assessment (PANDA) App on Antenatal Care Quality in Burkina Faso: Protocol for a Cluster Randomized Controlled Trial (Preprint)

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