Acute and sub-acute toxicity of a lyophilised aqueous extract of the aerial part of Spilanthes africana Delile in rats

Ngueguim, T. F.; Djouwoug, Clarice Noussi; Donfack, Jean Hubert; Gounoue, Kamkumo Raceline; Mbatchou, Adolphe; Kamtchouing, Pièrre; Dimo, Théophile

doi:10.1016/j.jep.2015.06.035

Cited by 7 publications

(2 citation statements)

References 12 publications

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“…Furthermore, it showed that some extracts from plants have the ability to alter body weight gain and organ weight parameters through their cumulative effects. Any changes of these parameters indicate a toxic effect of the plant extracts [ 23 ]. With that, the results suggested that the EOs extract of A.crassna did not produce any side effect on these parameters.…”

Section: Discussionmentioning

confidence: 99%

“…Commonly, the aqueous extracts seem overall to be less toxic than organic extracts, as the relative low toxicity in the aqueous extracts relies on the fact that they contain a wide class of phytochemical components in a similar way to their existence in natural form and hence, exhibit less risk of side effect [ 23 ]. The chemical analysis of hydrodistillation extract for A.crassna stem bark also demonstrated versatile classes of sesquiterpenes and polyphenols [ 15 ], and this is also true for the species of Aquilaria genus, in which these phytochemical components were identified as the main constituents [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

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In vivo toxicity and antitumor activity of essential oils extract from agarwood (Aquilaria crassna)

Dahham

Hassan

Ahamed

et al. 2016

BMC Complement Altern Med

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BackgroundAquilaria crassna has been used in traditional Asian medicine to treat vomiting, rheumatism, asthma, and cough. Furthermore, earlier studies from our laboratory have revealed that the essential oil extract from agarwood inhibited colorectal carcinoma cells. Despite of the wide range of ethno-pharmacological uses of agarwood, its toxicity has not been previously evaluated through systematic toxicological studies. Therefore, the potential safety of essential oil extract and its in vivo anti-tumor activity had been investigated.MethodsIn the acute toxicity study, Swiss female mice were given a single dose of the essential oil extract at 2000 mg/kg/day orally and screened for two weeks after administration. Meanwhile, in the sub-chronic study, two different doses of the extract were administered for 28 days. Mortality, clinical signs, body weight changes, hematological and biochemical parameters, gross findings, organ weights, and histological parameters were monitored during the study. Other than that, in vivo anti-tumor study was assessed by using subcutaneous tumors model established in nude mice.ResultsThe acute toxicity study showed that the LD50 of the extract was greater than 2000 mg/kg. In the repeated dose for 28-day oral toxicity study, the administration of 100 mg/kg and 500 mg/kg of essential oil per body weight revealed insignificant difference in food and water intakes, bodyweight change, hematological and biochemical parameters, relative organ weights, gross findings or histopathology compared to the control group. Nevertheless, the essential oil extract, when supplemented to nude mice, caused significant growth inhibition of the subcutaneous tumor of HCT 116 colorectal carcinoma cells.ConclusionCollectively, the data obtained indicated that essential oil extract from agarwood might be a safe material, and this essential oil is suggested as a potential anti-colon cancer candidate.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%