Acute and sub-chronic toxicity evaluations of aqueous extract from stem bark of Grewia mollis (Malvaceae) in rats

Pongri, Adarki; Igbe, Ighodaro

doi:10.1515/hepo-2017-0017

Cited by 3 publications

(3 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7,8,24 In the model plant which is induced GGPS gene as a structural enzyme in the terpenoid biosynthesis pathway. 12,13,14,25 In this study, the expression of two of the seven teak GGPS, namely the GGPS3 and GGPS6 genes have an expression pattern of up and down-regulation as part of the terpenoid biosynthesis process in young and old leaves. The results of DEG and qRT PCR gene expression analysis showed that GGPS6 maintained the up-regulation trend in the regulation of terpenoid formation.…”

Section: The Ggps3 and Ggps6 Expression Profiles In Teak Leavesmentioning

confidence: 88%

Identification of the Geranylgeranyl Pyrophosphate Synthase (GGPS) Gene Family in Teak (Tectona grandis)

2023

TJNPR

View full text Add to dashboard Cite

Teak (Tectona grandis, L.f.) plants are known to have the best quality wood in the Lamiaceae family but are also known to contain bioactive compounds that are important for pharmacology, both cosmetics and medicines. One of the phytochemical compounds contained in teak is retinol, which is important in cosmetic technology as an antiaging agent. Retinol is a derivative of carotene and carotene is a derivative of terpenoids. At the gene level, in model plants, the mechanism of terpenoid synthesis is controlled by a family of genes known as Geranylgeranyl Pyrophosphate Synthase (GGPS). The basic science of the GGPS gene is important to develop retinol production in teak. This research aims to identify the GGPS gene family in teak plants and observe the expression profile of two GGPS genes in teak plants, GGPS3 and GGPS6. The approach taken in this study used de novo NGS-transcriptome and qRT-PCR analysis. The results of this research were the results of transcriptome analysis, there were 87,365 contigs and seven GGPS gene sequences were identified. GGPS3 and GGPS6 were tested for expression profiles in young and old leaves using qRT-PCR. There is an up and down-regulation mechanism of the GGPS3 and GGPS6 genes in old and young leaves, indicating a feedback mechanism for these two genes. These results provide a strong basis for engineering terpenoid and retinol synthesis in teak plants.

show abstract

Section: The Ggps3 and Ggps6 Expression Profiles In Teak Leavesmentioning

confidence: 88%

Identification of the Geranylgeranyl Pyrophosphate Synthase (GGPS) Gene Family in Teak (Tectona grandis)

2023

TJNPR

View full text Add to dashboard Cite

show abstract

“…at day 0, day 7, day 14, day 21 and day 28. Untreated rats (group one) served as the control and was administered 2 ml of distilled water (Agu, 2016;Pongri and Igbe, 2017).…”

Section: Extract Administrationmentioning

confidence: 99%

Influence of Annona muricata (Soursop) on Colorectal Tissues of Wistar Rats.

Olubodun

Agu

2022

NJPS

View full text Add to dashboard Cite

Annona muricata (AMC), is a tropical plant species of the Annonaceae family known for its medicinal uses in ameliorating and/or protecting several organs and tissues in the body. In this study we elucidated the influence of the methanol extracts of AMC leaf and stem bark on the biochemical and histological integrity of the colorectum. Different doses (100, 200, 400, 600, and 800mg of extract/kg body weight) of methanol extracts of the leaves and stembark were orally administered to adult male Wistar rats of average weight (100 - 150 g) for 28 days. At the end of the experimental period, the rats were sacrificed and colorectal tissues harvested for analyses. Adenosine triphosphatase (ATPases), lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G6PD) activities, and tissue protein (TP) concentration, were evaluated by colorimetric method using appropriate test kits. The results in the colorectal tissue analysed showed that total ATPase, LDH and G6PD (energy markers) activities increased significantly in the groups which received methanol leaf and stem bark extracts of AMC when compared with control. However, a general decrease was observed in TP and Na+/K+-ATPase activity but there was a twist in Na+/K+-ATPase activity in stem bark doses of 200mg/kg and 400mg/kg which showed significant increases in dose dependent manner when compared with the control. Even though G6PD activity showed fluctuating patterns for both extracts, the highest dose (800mg/kg) reflected the most significant increase when compared with the control. The histology confirmed the toxicological results by the biochemical parameters. Under the condition of this study, we inferred that the methanol leaf and stem-bark extracts of AMC may significantly influence the enzymes by reducing and increasing total ATPase and Na+/K+-ATPase activities depending on the doses and administration should therefore depend on the desired outcome.

show abstract

“…10 Acute and subacute oral administration of stem bark for 28 days suggests it was relatively safe in rodents. 11 The stem bark and leaves are used to treat ulcers, rheumatism, and © 2023 the authors. This work is licensed under the Creative Commons Attribution 4.0 International License snakebites, diarrhea, obesity.…”

Section: Introductionmentioning

confidence: 99%

Antiatherogenic and Antiobesity Effects of Aqueous Stem Bark Extract of Grewia mollis Juss (Malvaceae)

2023

TJNPR

View full text Add to dashboard Cite

Grewia mollis Juss (Malvaceae) commonly described as "Dargaza" is a shrub, popularly used in folk medicine to treat obesity, diarrhoea and to ease childbirth. However, there are no established reports on its antiobesity activity in literature, hence, the aim of the study was to evaluate the antihyperlipidemic and antiobesity effects of Grewia mollis (GM) in rodents. Hyperlipidemia was induced by 200 mg/kg of triton WR-1339 and the rats were orally pretreated with extract (150-600 mg/kg) and atorvastatin (25 mg/kg) for 3 days. Obesity was induced with dietary cholesterol (600 mg/kg) for 10 weeks and rats were orally treated with extract (150-600 mg/kg). The effects of the extract on body weight, total food consumption, relative organ weight, Lee index, lipid profile, atherogenic index, coronary risk index and histopathological indices were evaluated. Preliminary phytochemical screening showed presence tannins, saponins, alkaloids, flavonoids and steroidal nucleus. The extract significantly (p<0.05) decreased total cholesterol, triglyceride, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol (VLDL), non-high-density lipoprotein cholesterol, atherogenic index, coronary risk index in hyperlipidemic rats. In the obese rat, there was significant (p<0.05) decrease in relative liver weight, body weight, Lee index, triglycerides, VLDL. Atherosclerotic changes in the coronary artery and aorta were ameliorated by the extract. The reduction in lipid profile and amelioration of atherosclerotic lesions indicate a possible anti-atherogenic property. These effects appear to be mediated via inhibition de novo triglyceride and cholesterol biosynthesis. The extract shows therapeutic promise in the management of obesity, hyperlipidemia and in the prevention of atherosclerosis.

show abstract

Acute and sub-chronic toxicity evaluations of aqueous extract from stem bark of Grewia mollis (Malvaceae) in rats

Cited by 3 publications

References 15 publications

Identification of the Geranylgeranyl Pyrophosphate Synthase (GGPS) Gene Family in Teak (Tectona grandis)

Identification of the Geranylgeranyl Pyrophosphate Synthase (GGPS) Gene Family in Teak (Tectona grandis)

Influence of Annona muricata (Soursop) on Colorectal Tissues of Wistar Rats.

Antiatherogenic and Antiobesity Effects of Aqueous Stem Bark Extract of Grewia mollis Juss (Malvaceae)

Contact Info

Product

Resources

About