Acute Boldine Treatment Induces Anti-convulsant Effects in Mice through its Antioxidant Activity

Abstract

Boldine is a natural antioxidant that exhibits some important pharmacological properties, which is due to its free radical scavenging effects. And at the same time, reactive oxygen species (ROS) has an important role in pathogenesis of seizure; hence, reducing it via antioxidants like boldine seems to be effective in treating seizure. This study was designed to investigate whether acute treatment with boldine could alter seizures induced by pentylenetetrazole or electroshock in mice. We also evaluated to see if boldine's antioxidant properties play a role in its anti-convulsant activity. Boldine acute administration increased time latencies to the onset of myoclonic jerks and clonic seizures induced by intraperitoneal pentylenetetrazole model. Moreover, boldine increased seizure threshold induced by intravenous infusion of pentylenetetrazole. Additionally, acute doses of boldine reduced the duration of tonic hind-limb extension in the electroshock-induced seizure model. Non-effective dose of vitamin C (as an antioxidant agent) and boldine had anti-convulsant effect in intraperitoneal pentylenetetrazole, intravenous pentylenetetrazole and electroshock models. Boldine administration increased glutathione and superoxide dismutase levels in mice whole brain. The result showed boldine anti-seizure properties, which might be due to its antioxidant activity.

Boldine treatment protects acetaminophen‐induced liver inflammation and acute hepatic necrosis in mice

“…Boldine, an alkaloid obtained from boldo leaves (Peumus boldus Mol) was reported to have a variety of health benefits including glutathione replenishment, free radical scavenging, anti-inflammatory, antidiabetic, and anticancer effects by virtue of its antioxidant properties. [19][20][21][22][23] In the liver context, the hepatoprotective effect of boldine has been studied against bile-duct ligation induced hepatic fibrosis and cirrhosis in rats. [24] In a study, boldine treatments prevented hepatotoxicity induced by carbon tetrachloride in rats.…”

Integration of Gut Microbiota, Serum Metabolomic, and Network Pharmacology to Reveal the Anti Insomnia Mechanism of Mongolian Medicine Sugemule-4 Decoction on Insomnia Model Rats

“…The findings suggested that different components of MMS-4D showed curative effects against insomnia by regulating different targets and pathways. Among these ingredients, tetrahydroberberine 17 and boldine 18 could be used to treat insomnia, thereby confirming the therapeutic value of MMS-4D for this disease.…”

Harnessing the power of natural alkaloids: the emergent role in epilepsy therapy

“…Furthermore, boldine's long-term administration has been associated with augmented levels of glutathione and superoxide dismutase, reflecting its intrinsic antioxidant capabilities (Akotkar et al, 2023). Experimental data prove, Acute administration of noneffective dose of boldine (10 mg/kg) and vitamin C (50 mg/kg) induced anti-convulsing effects of boldine on seizure threshold (Moezi et al, 2019). Its free radical neutralizing activity, especially against hydroxyl radicals, positions boldine as a candidate of considerable interest for epilepsy treatment strategies.…”

“…Its free radical neutralizing activity, especially against hydroxyl radicals, positions boldine as a candidate of considerable interest for epilepsy treatment strategies. These viewpoints collectively expand the treatment landscape for alkaloids in epilepsy management (Moezi et al, 2019). Pyridine alkaloids can also exert their effects by inhibiting synaptic transmission, and tetramethylpyrazine, a pyridine alkaloid derived from Conioselinum anthriscoides (H. Boissieu) Pimenov, is one such compound recognized for its antiseizure properties in clinical settings (Lin J. et al, 2022).…”