Acute Cholecystitis

Ulreich, Sidney; Foster, Kendall W.; Stier, Steven A.; Rosenfield, Arthur T.

doi:10.1001/archsurg.1980.01380020026006

Cited by 16 publications

(2 citation statements)

References 8 publications

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“…A similar protective effect of LP pretreatment was seen in cells treated with EGTA (Figure 2B). LPS treatment also increased permeability compared to LR-pretreated or Control cells [p<0.0024], and LR pretreatment blunted the effect of LPS (p<0.015; Figure 2C) [28]. Similarly, LPS increased permeability compared to LP-pretreated or Control cells [p<0.0024] and LP pretreatment decreased the effect of LPS (p<0.005; Figure 2D).…”

Section: Resultsmentioning

confidence: 99%

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Probiotic Lactobacillus Species Strengthen Intestinal Barrier Function and Tight Junction Integrity in Experimental Necrotizing Enterocolitis

et al. 2017

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Necrotizing enterocolitis (NEC) is a serious intestinal disease that occurs in newborn infants. It is associated with major morbidity and affects 5% of all infants admitted to neonatal intensive care units. Probiotics have variable efficacy in preventing necrotizing enterocolitis. Tight junctions (TJ) are protein complexes that maintain epithelial barrier integrity. We hypothesized that the probiotics Lactobacillus rhamnosus and Lactobacillus plantarum strengthen intestinal barrier function, promote TJ integrity, and protect against experimental NEC. Both an in vitro and an in vivo experimental model of NEC were studied. Cultured human intestinal Caco-2 cells were pretreated with L. rhamnosus and L. plantarum probiotics. TJ were then disrupted by EGTA calcium switch or LPS to mimic NEC in vitro. Trans-epithelial resistance (TER) and flux of fluorescein isothiocynate dextran was measured. TJ structure was evaluated by ZO-1 immunofluorescence. In vivo effects of ingested probiotics on intestinal injury and ZO-1 expression were assessed in a rat model of NEC infected with Cronobacter sakazakii (CS). Caco-2 cells treated with individual probiotics demonstrated higher TER and lower permeability compared to untreated cells (p<0.0001). ZO-1 immunofluorescence confirmed TJ stability in treated cells. Rat pups fed probiotics alone had more intestinal injury compared with controls (p=0.0106). Probiotics were protective against injury when given in combination with CS, with no difference in intestinal injury compared to controls (p=0.21). Increased permeability was observed in the probiotic and CS groups (p=0.03, p=0.05), but not in the probiotic plus CS group (p=0.79). Lactobacillus sp. strengthened intestinal barrier function and preserved TJ integrity in an in vitro experimental model of NEC. In vivo, probiotic bacteria were not beneficial when given alone, but were protective in the presence of CS in a rat model of NEC.

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Section: Resultsmentioning

confidence: 99%

“…L. rhamnosus (ATCC 53103) [28] [25] and L. plantarum (ATCC 10241) were cultured in MRS media overnight culture to a density of 10 8 CFU/mL before being used as described in the experiments. The final concentration of bacteria in experiments was 10 7 CFU/mL.…”

Section: Methodsmentioning

confidence: 99%