Acute cold stress induces transient MuRF1 upregulation in the skeletal muscle of zebrafish

Tamai, Shinsuke; Fujita, Shin‐ichiro; Komine, Ritsuko; Kanki, Yasuharu; Aoki, Kai; Watanabe, Koichi; Takekoshi, Kazuhiro; Sugasawa, Takehito

doi:10.1016/j.bbrc.2022.03.093

Cited by 5 publications

(4 citation statements)

References 28 publications

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“…Skeletal muscle is a major determinant of systemic energy homeostasis because of its large mass and high rate of fuel consumption. Some genes related to muscle fibre type conversion (Kim et al., 2021 ), promotion of protein processing (Tamai et al., 2022 ), and white adipose browning (Lee et al., 2014 ) play important roles in skeletal muscle heat production under cold exposure. However, RNA‐seq technology, which can screen for major genes and biology pathways at the transcriptome level, has not been widely used to study the molecular mechanism of pig responses to low temperature.…”

Section: Discussionmentioning

confidence: 99%

“…Skeletal muscle is a major determinant of systemic energy homeostasis because of its large mass and high rate of fuel consumption. Some genes related to muscle fibre type conversion (Kim et al, 2021), promotion of protein processing (Tamai et al, 2022), and white adipose browning (Lee et al, of yellow croaker (H. Xu et al, 2015). ELOVL6 is a rate-limiting enzyme catalyzing elongation of saturated and monounsaturated long-chain fatty acids.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of pig skeletal muscle transcriptomes in response to low temperature

Dong-jie

Wang

et al. 2022

Veterinary Medicine & Sci

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Objectives The response of mammals to cold environment is a complex physiological activity, and its underlying mechanism must be analyzed from multiple perspectives. Skeletal muscle is an important thermogenic tissue that maintains body temperature in mammals. We dissected the molecular mechanism of pig skeletal muscle response to a cold environment by performing comparative transcriptome analysis in the Enshi black pig. Methods Three pigs were subjected to acute cold stress (3 days), three pigs were subjected to cold acclimation (58 days), and three pigs were used as controls. RNA‐seq was used to screen the differentially expressed genes (DEGs) of skeletal muscle. Results Using RNA‐seq methods, we identified 1241 DEGs within the acute cold stress group and 1886 DEGs within the cold acclimation group. Prolonged cold exposure induced more gene expression changes. A total of 540 key cold‐responsive DEGs were found, and their trends were consistent within the acute cold stress group and cold acclimation group. Gene expression pattern analysis showed that there were significant differences between the low‐temperature treatment groups and the control group, and there were also differences between individuals after long‐term low‐temperature treatment. Analysis of DEGs revealed that 134 pathways were significantly enriched in the cold adaptation group, 98 pathways were significantly enriched in the acute cold stress group, and 71 pathways were shared between the two groups. The 71 shared pathways were mainly related to lipid, amino acid, and carbohydrate metabolism; signal transduction; endocrine, immune, and nervous system; cardiovascular disease; infectious diseases caused by bacteria or viruses; and neurodegenerative disease. Conclusions In conclusion, this study provides insights into the molecular mechanism of porcine skeletal muscle response under low‐temperature environment. The data may assist further research on the mechanism of pig response to cold exposure.

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Section: Discussionmentioning

confidence: 99%

“…Skeletal muscle is a major determinant of systemic energy homeostasis because of its large mass and high rate of fuel consumption. Some genes related to muscle fibre type conversion (Kim et al, 2021), promotion of protein processing (Tamai et al, 2022), and white adipose browning (Lee et al, of yellow croaker (H. Xu et al, 2015). ELOVL6 is a rate-limiting enzyme catalyzing elongation of saturated and monounsaturated long-chain fatty acids.…”

Section: Discussionmentioning

confidence: 99%

Characterization of pig skeletal muscle transcriptomes in response to low temperature

Dong-jie

Wang

et al. 2022

Veterinary Medicine & Sci

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“…Samples were prepared for each group to perform three independent assays. mRNA sequencing was performed using Illumina NextSeq500 (Illumina, Inc.) at the Department of Sports Medicine Analysis under the Open Facility Network Office, University of Tsukuba, in accordance with the standard protocol ( 34 ). Sequencing was conducted with paired-end reads of 36 bases.…”

Section: Methodsmentioning

confidence: 99%

High expression of Sam68 contributes to metastasis by regulating vimentin expression and a motile phenotype in oral squamous cell carcinoma

et al. 2022

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The present study aimed to investigate the clinical and biological significance of Src-associated in mitosis 68 kDa (Sam68) in oral squamous cell carcinoma (OSCC). Immunohistochemical analysis was performed on tissue samples obtained from 77 patients with OSCC. Univariate analysis revealed that the high expression of Sam68 was significantly correlated with advanced pathological T stage (P=0.01), positive lymphovascular invasion (P=0.01), and pathological cervical lymph node metastasis (P<0.01).Moreover, multivariate analysis demonstrated that the high expression of Sam68 was an independent predictive factor for cervical lymph node metastasis (odds ratio, 4.39; 95% confidence interval, 1.49-14.23; P<0.01). These results indicated that high Sam68 expression contributed to tumor progression, especially cervical lymph node metastasis, in OSCC. mRNA sequencing was also performed to assess the changes in the transcriptome between OSCC cells with Sam68 knockdown and control cells with the aim of elucidating the biological roles of Sam68. Gene Ontology enrichment analysis revealed that downregulated differentially expressed genes (DEGs) were concentrated in some biological processes related to epithelial-mesenchymal transition. Among these DEGs, it was established that vimentin was particularly downregulated in these cells. It was also confirmed that Sam68 knockdown reduced the motility of OSCC cells. Furthermore, the immunohistochemical study of vimentin identified the association between vimentin expression and Sam68 expression as well as cervical lymph node metastasis. In conclusion, the present study suggested that the high expression of Sam68 may contribute to metastasis by regulating vimentin expression and a motile mesenchymal phenotype in OSCC.

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“…Temporal analysis indicates a transient enhancement in the expression of E3 ubiquitin ligases, notably the muscle RINGfinger protein-1 (MuRF1). This temporal modulation offers valuable information about the potential advantages and risks associated with cryotherapy for the management of muscle trauma or fatigue, revealing a temporary increase in the expression of E3 ubiquitin ligases, particularly MuRF1 [16]. These results of zebrafish research provide a template for personalized treatment in muscle-related disorders such as sarcopenia.…”

Section: Zebrafish As An Animal Model Of Primary Sarcopeniamentioning

confidence: 95%

Zebrafish as an Emerging Model for Sarcopenia: Considerations, Current Insights, and Future Directions

Callegari,

Mirzaei,

Agbaria

et al. 2023

IJMS

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Sarcopenia poses a significant challenge to public health and can severely impact the quality of life of aging populations. Despite extensive efforts to study muscle degeneration using traditional animal models, there is still a lack of effective diagnostic tools, precise biomarkers, and treatments for sarcopenia. Zebrafish models have emerged as powerful tools in biomedical research, providing unique insights into age-related muscle disorders like sarcopenia. The advantages of using zebrafish models include their rapid growth outside of the embryo, optical transparency during early developmental stages, high reproductive potential, ease of husbandry, compact size, and genetic tractability. By deepening our understanding of the molecular processes underlying sarcopenia, we may develop novel diagnostic tools and effective treatments that can improve the lives of aging individuals affected by this condition. This review aims to explore the unique advantages of zebrafish as a model for sarcopenia research, highlight recent breakthroughs, outline potential avenues for future investigations, and emphasize the distinctive contributions that zebrafish models offer. Our research endeavors to contribute significantly to address the urgent need for practical solutions to reduce the impact of sarcopenia on aging populations, ultimately striving to enhance the quality of life for individuals affected by this condition.

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Acute cold stress induces transient MuRF1 upregulation in the skeletal muscle of zebrafish

Cited by 5 publications

References 28 publications

Characterization of pig skeletal muscle transcriptomes in response to low temperature

Characterization of pig skeletal muscle transcriptomes in response to low temperature

High expression of Sam68 contributes to metastasis by regulating vimentin expression and a motile phenotype in oral squamous cell carcinoma

Zebrafish as an Emerging Model for Sarcopenia: Considerations, Current Insights, and Future Directions

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